CDC5L

Cell division cycle 5-like protein is a protein that in humans is encoded by the CDC5L gene.[5][6][7]

CDC5L
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DIM, 2DIN
Identifiers
Aliases	CDC5L, CDC5, CDC5-LIKE, CEF1, PCDC5RP, dJ319D22.1, cell division cycle 5 like
External IDs	OMIM: 602868 MGI: 1918952 HomoloGene: 13291 GeneCards: CDC5L
Gene location (Human) Chr. Chromosome 6 (human)[1] Band 6p21.1 Start 44,387,706 bp[1] End 44,450,425 bp[1]
Gene location (Mouse) Chr. Chromosome 17 (mouse)[2] Band 17|17 B3 Start 45,391,884 bp[2] End 45,433,737 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • protein phosphatase 1 binding • leucine zipper domain binding • WD40-repeat domain binding • GO:0001948 protein binding • transcription corepressor binding • protein kinase binding • sequence-specific DNA binding • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific • RNA binding • RNA polymerase II regulatory region sequence-specific DNA binding • GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA polymerase II-specific Cellular component • cytoplasm • nuclear speck • catalytic step 2 spliceosome • membrane • Prp19 complex • nucleoplasm • protein-DNA complex • perinuclear region of cytoplasm • DNA replication factor A complex • spliceosomal complex • cell nucleus • U2-type catalytic step 2 spliceosome Biological process • mRNA splicing, via spliceosome • cellular response to nerve growth factor stimulus • regulation of transcription, DNA-templated • mRNA processing • cellular response to fibroblast growth factor stimulus • transcription, DNA-templated • cellular response to interleukin-2 • cellular response to prolactin • cellular response to DNA damage stimulus • signal transduction involved in DNA damage checkpoint • cellular response to wortmannin • GO:0007067 mitotic cell cycle • cell cycle • DNA repair • cell differentiation • RNA splicing • regulation of transcription from RNA polymerase II promoter • transcription from RNA polymerase II promoter • positive regulation of transcription from RNA polymerase II promoter Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	988	71702
Ensembl	ENSG00000096401	ENSMUSG00000023932
UniProt	Q99459	Q6A068
RefSeq (mRNA)	NM_001253	NM_152810
RefSeq (protein)	NP_001244	NP_690023
Location (UCSC)	Chr 6: 44.39 – 44.45 Mb	Chr 17: 45.39 – 45.43 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G₂/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G₂/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.[7]

Interactions

CDC5L has been shown to interact with:

• ASF/SF2,[8]
• BZW1,[8]
• CWC15,[8]
• DNA-PKcs,[8]
• DYNC1H1,[8]
• GCN1L1,[8]
• HSPA8,[8]
• ILF2,[8]
• PLRG1,[8][9][10][11]
• PPM1D,[8]
• PPP1CA,[8]
• PRPF19,[8]
• RBMX[8] and
• RPL12,[8]
• RPL13,[8]
• RPS16,[8]
• RPS25,[8]
• SF3A1,[8]
• SF3B1,[8]
• SF3B2,[8]
• SF3B4,[8]
• SFPQ,[8]
• SFRS2,[8]
• SNRPA1,[8]
• SNRPD3,[8]
• SRRM1,[8]
• Small nuclear ribonucleoprotein D1,[8]
• Small nuclear ribonucleoprotein D2,[8]
• Small nuclear ribonucleoprotein polypeptide A,[8]
• TOP2A,[8] and
• TTF2.[12]

See also

• G2/M checkpoint

References

1. GRCh38: Ensembl release 89: ENSG00000096401 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000023932 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Groenen PM, Vanderlinden G, Devriendt K, Fryns JP, Van de Ven WJ (Apr 1998). "Rearrangement of the human CDC5L gene by a t(6;19)(p21;q13.1) in a patient with multicystic renal dysplasia". Genomics. 49 (2): 218–29. doi:10.1006/geno.1998.5254. PMID 9598309.
6. Bernstein HS, Coughlin SR (Feb 1997). "Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling". The Journal of Biological Chemistry. 272 (9): 5833–7. doi:10.1074/jbc.272.9.5833. PMID 9038199.
7. "Entrez Gene: CDC5L CDC5 cell division cycle 5-like (S. pombe)".
8. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
9. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
10. Ajuh P, Lamond AI (Nov 2003). "Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1". Nucleic Acids Research. 31 (21): 6104–16. doi:10.1093/nar/gkg817. PMC 275459. PMID 14576297.
11. Ajuh P, Sleeman J, Chusainow J, Lamond AI (Nov 2001). "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing". The Journal of Biological Chemistry. 276 (45): 42370–81. doi:10.1074/jbc.M105453200. PMID 11544257.
12. Leonard D, Ajuh P, Lamond AI, Legerski RJ (Sep 2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing". Biochemical and Biophysical Research Communications. 308 (4): 793–801. CiteSeerX 10.1.1.539.8359. doi:10.1016/s0006-291x(03)01486-4. PMID 12927788.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. CiteSeerX 10.1.1.500.923. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Heaney JP, Jacobson A (Aug 1975). "Simplified control of upper abdominal hemorrhage from the vena cava". Surgery. 78 (2): 138–41. PMID 1154257.
• Hirayama T, Shinozaki K (Nov 1996). "A cdc5+ homolog of a higher plant, Arabidopsis thaliana". Proceedings of the National Academy of Sciences of the United States of America. 93 (23): 13371–6. doi:10.1073/pnas.93.23.13371. PMC 24100. PMID 8917598.
• Ishikawa K, Nagase T, Nakajima D, Seki N, Ohira M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Oct 1997). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 4 (5): 307–13. doi:10.1093/dnares/4.5.307. PMID 9455477.
• Bernstein HS, Coughlin SR (Feb 1998). "A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entry". The Journal of Biological Chemistry. 273 (8): 4666–71. doi:10.1074/jbc.273.8.4666. PMID 9468527.
• Ohi R, Feoktistova A, McCann S, Valentine V, Look AT, Lipsick JS, Gould KL (Jul 1998). "Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes". Molecular and Cellular Biology. 18 (7): 4097–108. doi:10.1128/mcb.18.7.4097. PMC 108994. PMID 9632794.
• Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (Sep 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
• Burns CG, Ohi R, Krainer AR, Gould KL (Nov 1999). "Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing". Proceedings of the National Academy of Sciences of the United States of America. 96 (24): 13789–94. doi:10.1073/pnas.96.24.13789. PMC 24143. PMID 10570151.
• Boudrez A, Beullens M, Groenen P, Van Eynde A, Vulsteke V, Jagiello I, Murray M, Krainer AR, Stalmans W, Bollen M (Aug 2000). "NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry". The Journal of Biological Chemistry. 275 (33): 25411–7. doi:10.1074/jbc.M001676200. PMID 10827081.
• Lei XH, Shen X, Xu XQ, Bernstein HS (Dec 2000). "Human Cdc5, a regulator of mitotic entry, can act as a site-specific DNA binding protein". Journal of Cell Science. 113. 113 (24): 4523–31. PMID 11082045.
• Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
• Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M (May 2001). "Creation of genome-wide protein expression libraries using random activation of gene expression". Nature Biotechnology. 19 (5): 440–5. doi:10.1038/88107. PMID 11329013.
• Ajuh P, Sleeman J, Chusainow J, Lamond AI (Nov 2001). "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing". The Journal of Biological Chemistry. 276 (45): 42370–81. doi:10.1074/jbc.M105453200. PMID 11544257.
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (Apr 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
• Leonard D, Ajuh P, Lamond AI, Legerski RJ (Sep 2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing". Biochemical and Biophysical Research Communications. 308 (4): 793–801. CiteSeerX 10.1.1.539.8359. doi:10.1016/S0006-291X(03)01486-4. PMID 12927788.
• Liu L, Gräub R, Hlaing M, Epting CL, Turck CW, Xu XQ, Zhang L, Bernstein HS (2004). "Distinct domains of human CDC5 direct its nuclear import and association with the spliceosome". Cell Biochemistry and Biophysics. 39 (2): 119–32. doi:10.1385/CBB:39:2:119. PMID 14515018.

External links

• Human CDC5L genome location and CDC5L gene details page in the UCSC Genome Browser.