(S)-2-haloacid dehalogenase

In enzymology, a (S)-2-haloacid dehalogenase (EC 3.8.1.2) is an enzyme that catalyzes the chemical reaction

(S)-2-haloacid + H2O (R)-2-hydroxyacid + halide
(S)-2-haloacid dehalogenase
Identifiers
EC number3.8.1.2
CAS number37289-39-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Thus, the two substrates of this enzyme are (S)-2-haloacid and H2O, whereas its two products are (R)-2-hydroxyacid and halide.

This enzyme belongs to the family of hydrolases, specifically those acting on halide bonds in carbon-halide compounds. The systematic name of this enzyme class is (S)-2-haloacid halidohydrolase. Other names in common use include 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase [ambiguous][ambiguous], 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DL-2-haloacid dehalogenase[ambiguous], L-2-haloacid dehalogenase, and L-DEX. This enzyme participates in gamma-hexachlorocyclohexane degradation and 1,2-dichloroethane degradation.

Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1AQ6, 1JUD, 1QH9, 1QQ5, 1QQ6, 1QQ7, 1ZRM, 1ZRN, 2NO4, and 2NO5.

gollark: Apiowhat?
gollark: A square wave is apparently in some confusing way equivalent to the sum of an infinite number of sine waves, so you get horrible interference, and it's low-power so the range is terrible.
gollark: It can generate ~100MHz square waves and you can connect up an antenna, which is *basically* what a radio transmitter would do but stupider and worse.
gollark: Yes, a clock or something.
gollark: A quirk of the raspberry pi means it can transmit FM radio with horrible interference because it can only broadcast square waves or something, because of happening to have a somewhat adjustable ~100MHz clock exposed on external pins or something.

References
    • Goldman P, Milne GW, Keister DB (1968). "Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases". J. Biol. Chem. 243 (2): 428–34. PMID 5635785.
    • Motosugi M, Esaki N, Soda K (1982). "Preparation and properties of 2-halo acid dehalogenase from Pseudomonas putida". Agric. Biol. Chem. 46 (3): 837–838. doi:10.1271/bbb1961.46.837.
    • Klages U, Krauss S, Lingens F (1983). "2-Haloacid dehalogenase from a 4-chlorobenzoate-degrading Pseudomonas spec. CBS 3". Hoppe-Seyler's Z. Physiol. Chem. 364 (5): 529–35. doi:10.1515/bchm2.1983.364.1.529. PMID 6873881.
    • Diez A, Prieto MI, Alvarez MJ, Bautista JM, Garrido J, Puyet A (1996). "Improved catalytic performance of a 2-haloacid dehalogenase from Azotobacter sp. by ion-exchange immobilisation". Biochem. Biophys. Res. Commun. 220 (3): 828–33. doi:10.1006/bbrc.1996.0489. PMID 8607850.
    • Morsberger FM, Muller R, Otto MK, Lingens F, Kulbe KD (1991). "Purification and characterization of 2-halocarboxylic acid dehalogenase II from Pseudomonas spec. CBS 3". Biol. Chem. Hoppe-Seyler. 372 (10): 915–22. doi:10.1515/bchm3.1991.372.2.915. PMID 1772590.
    • Kohler R, Brokamp A, Schwarze R, Reiting RH, Schmidt FR (1998). "Characteristics and DNA-sequence of a cryptic haloalkanoic acid dehalogenase from Agrobacterium tumefaciens RS5". Curr. Microbiol. 36 (2): 96–101. doi:10.1007/s002849900286. PMID 9425247.
    • Motosugi K, Esaki N, Soda K (1982). "Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase". Arch. Microbiol. 131 (3): 179–83. doi:10.1007/BF00405875. PMID 7103659.
    • Kurihara T, Esaki N, Soda K (2000). "Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms". J. Mol. Catal., B Enzym. 10 (1–3): 57–65. doi:10.1016/S1381-1177(00)00108-9.
    • M, Tsunasawa S; Esaki N (1996). "Bacterial 2-haloacid dehalogenases: Structures and catalytic properties". Pure Appl. Chem. 68 (11): 2097–2103. doi:10.1351/pac199668112097.


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