Von Kossa stain

The von Kossa histological stain is used to quantify mineralization in cell culture and histological sections.

Method

This is a staining method to illustrates mineralization such as Calcium and Potassium in tissues.[1][2][3][4][5]

It is a precipitation reaction in which silver ions react with phosphate in the presence of acidic material.[6] Photo chemical degradation of silver phosphate to silver happens under light illumination.

Additional methods need to be employed to confirm the presence of calcium, such as Alizarin Red S.

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References
  1. von Kossa J (1901) Ueber die im Organismus kunstlich erzeugbaren Verkalkungen. Beit Path Anat 29:163
  2. Clark G (1981) Staining Procedures. Williams and Wilkins, Baltimore, MD, pp 187
  3. Lillie R, Fuller H (1976) Histopathologic technique and practical histochemistry. McGraw-Hill, New York
  4. Mallory FB (1983) Pathological techniques: A practical manual for workers in pathological histology including directions for the performance of autopsies and for microphotography. In: Mallory NY (eds) WB Saunders, Philadelphia, PA, pp 143–144
  5. Drury, R. A. B.; Wallington, E. A. (1980). Carleton's Histological Technique (5th ed.). Oxford University Press. p. 520. ISBN 0-19-261310-3.
  6. Meloan, Susan N.; Puchtler, Holde (2013). "Chemical Mechanisms of Staining Methods: Von Kossa's Technique: What von Kossa Really Wrote and a Modified Reaction for Selective Demonstration of Inorganic Phosphates". Journal of Histotechnology. 8 (1): 11–13. doi:10.1179/his.1985.8.1.11. ISSN 0147-8885.
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