USP14

Ubiquitin-specific protease 14 is an enzyme that in humans is encoded by the USP14 gene.[5][6]

USP14
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2AYN, 2AYO
Identifiers
Aliases	USP14, TGT, ubiquitin specific peptidase 14
External IDs	OMIM: 607274 MGI: 1928898 HomoloGene: 3780 GeneCards: USP14
Gene location (Human) Chr. Chromosome 18 (human)[1] Band 18p11.32 Start 158,383 bp[1] End 214,629 bp[1]
Gene location (Mouse) Chr. Chromosome 18 (mouse)[2] Band 18 A1|18 4.91 cM Start 9,993,066 bp[2] End 10,045,119 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • cysteine-type peptidase activity • proteasome binding • peptidase activity • GO:0001948 protein binding • thiol-dependent ubiquitin-specific protease activity • cysteine-type endopeptidase activity • endopeptidase inhibitor activity • hydrolase activity • tRNA guanylyltransferase activity • thiol-dependent ubiquitinyl hydrolase activity Cellular component • membrane • cell membrane • synapse • cell surface • proteasome complex • extracellular exosome • cytoplasm • cytosol • cytoplasmic vesicle • cell nucleus Biological process • regulation of proteasomal protein catabolic process • regulation of chemotaxis • ubiquitin-dependent protein catabolic process • proteolysis • negative regulation of ER-associated ubiquitin-dependent protein catabolic process • chemical synaptic transmission • negative regulation of endopeptidase activity • protein deubiquitination • immune system process • innate immune system • tRNA 5'-end processing Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9097	59025
Ensembl	ENSG00000101557	ENSMUSG00000047879
UniProt	P54578	Q9JMA1
RefSeq (mRNA)	NM_005151 NM_001037334	NM_001038589 NM_021522 NM_001360884
RefSeq (protein)	NP_001032411 NP_005142	NP_001033678 NP_067497 NP_001347813
Location (UCSC)	Chr 18: 0.16 – 0.21 Mb	Chr 18: 9.99 – 10.05 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.[6]

Interactions

USP14 has been shown to interact with CXCR4.[7]

References

1. GRCh38: Ensembl release 89: ENSG00000101557 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000047879 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Puente XS, Sánchez LM, Overall CM, López-Otín C (Jul 2003). "Human and mouse proteases: a comparative genomic approach". Nat Rev Genet. 4 (7): 544–58. doi:10.1038/nrg1111. PMID 12838346.
6. "Entrez Gene: USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)".
7. Mines MA, Goodwin JS, Limbird LE, Cui FF, Fan GH (Feb 2009). "Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK activation". J. Biol. Chem. 284 (9): 5742–52. doi:10.1074/jbc.M808507200. PMC 2645827. PMID 19106094.

Further reading

• Wilson SM, Bhattacharyya B, Rachel RA, Coppola V, Tessarollo L, Householder DB, Fletcher CF, Miller RJ, Copeland NG, Jenkins NA (2002). "Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease". Nature Genetics. 32 (3): 420–425. doi:10.1038/ng1006. PMID 12368914.
• D'Andrea A, Pellman D (1999). "Deubiquitinating enzymes: a new class of biological regulators". Crit. Rev. Biochem. Mol. Biol. 33 (5): 337–52. doi:10.1080/10409239891204251. PMID 9827704.
• Deshpande KL, Seubert PH, Tillman DM, Farkas WR, Katze JR (1996). "Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit". Arch. Biochem. Biophys. 326 (1): 1–7. doi:10.1006/abbi.1996.0039. PMID 8579355.
• Reuter TY, Medhurst AL, Waisfisz Q, Zhi Y, Herterich S, Hoehn H, Gross HJ, Joenje H, Hoatlin ME, Mathew CG, Huber PA (2003). "Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport". Exp. Cell Res. 289 (2): 211–21. doi:10.1016/S0014-4827(03)00261-1. PMID 14499622.
• Dennehey BK, Gutches DG, McConkey EH, Krauter KS (2004). "Inversion, duplication, and changes in gene context are associated with human chromosome 18 evolution". Genomics. 83 (3): 493–501. doi:10.1016/j.ygeno.2003.08.017. PMID 14962675.
• Hu M, Li P, Song L, Jeffrey PD, Chenova TA, Wilkinson KD, Cohen RE, Shi Y (2005). "Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14". EMBO J. 24 (21): 3747–56. doi:10.1038/sj.emboj.7600832. PMC 1276716. PMID 16211010.
• Shinji S, Naito Z, Ishiwata S, Ishiwata T, Tanaka N, Furukawa K, Suzuki H, Seya T, Matsuda A, Katsuta M, Tajiri T (2006). "Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases". Oncol. Rep. 15 (3): 539–43. doi:10.3892/or.15.3.539. PMID 16465409.
• Crimmins S, Jin Y, Wheeler C, Huffman AK, Chapman C, Dobrunz LE, Levey A, Roth KA, Wilson JA, Wilson SM (2006). "Transgenic rescue of ataxia mice with neuronal-specific expression of ubiquitin-specific protease 14". J. Neurosci. 26 (44): 11423–31. doi:10.1523/JNEUROSCI.3600-06.2006. PMID 17079671.

External links

• Overview of all the structural information available in the PDB for UniProt: P54578 (Ubiquitin carboxyl-terminal hydrolase 14) at the PDBe-KB.