TLK1

Serine/threonine-protein kinase tousled-like 1 is an enzyme that in humans is encoded by the TLK1 gene.[5][6][7]

TLK1
Identifiers
Aliases	TLK1, PKU-beta, tousled like kinase 1
External IDs	OMIM: 608438 MGI: 2441683 HomoloGene: 130657 GeneCards: TLK1
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q31.1 Start 170,990,823 bp[1] End 171,231,314 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 C2 Start 70,712,407 bp[2] End 70,825,728 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • nucleotide binding • protein serine/threonine kinase activity • protein kinase activity • GO:0001948 protein binding • ATP binding • kinase activity Cellular component • cell nucleus Biological process • protein phosphorylation • cell cycle • GO:0007243 intracellular signal transduction • intracellular protein transport • regulation of chromatin assembly or disassembly • phosphorylation • cellular response to DNA damage stimulus • chromatin organization • chromosome segregation • peptidyl-serine phosphorylation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9874	228012
Ensembl	ENSG00000198586	ENSMUSG00000041997
UniProt	Q9UKI8	Q8C0V0
RefSeq (mRNA)	NM_001136554 NM_001136555 NM_012290	NM_172664 NM_001362963
RefSeq (protein)	NP_001130026 NP_001130027 NP_036422	NP_766252 NP_001349892
Location (UCSC)	Chr 2: 170.99 – 171.23 Mb	Chr 2: 70.71 – 70.83 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The Tousled-like kinases, first described in Arabidopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM][7]

Interactions

TLK1 has been shown to interact with ASF1B,[8][9] ASF1A[8][10] and TLK2.[11]

References

1. GRCh38: Ensembl release 89: ENSG00000198586 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000041997 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Yamakawa A, Kameoka Y, Hashimoto K, Yoshitake Y, Nishikawa K, Tanihara K, Date T (Nov 1997). "cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal". Gene. 202 (1–2): 193–201. doi:10.1016/S0378-1119(97)00495-2. PMID 9427565.
6. Groth A, Lukas J, Nigg EA, Silljé HH, Wernstedt C, Bartek J, Hansen K (Apr 2003). "Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint". The EMBO Journal. 22 (7): 1676–87. doi:10.1093/emboj/cdg151. PMC 152895. PMID 12660173.
7. "Entrez Gene: TLK1 tousled-like kinase 1".
8. Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases". Current Biology. 11 (13): 1068–73. doi:10.1016/S0960-9822(01)00298-6. PMID 11470414.
9. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
10. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
11. Silljé HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA (Oct 1999). "Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication". The EMBO Journal. 18 (20): 5691–702. doi:10.1093/emboj/18.20.5691. PMC 1171636. PMID 10523312.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. CiteSeerX 10.1.1.500.923. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Schultz SJ, Nigg EA (Oct 1993). "Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans". Cell Growth & Differentiation. 4 (10): 821–30. PMID 8274451.
• Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N (Aug 1995). "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA Research. 2 (4): 167–74, 199–210. doi:10.1093/dnares/2.4.167. PMID 8590280.
• Silljé HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA (Oct 1999). "Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication". The EMBO Journal. 18 (20): 5691–702. doi:10.1093/emboj/18.20.5691. PMC 1171636. PMID 10523312.
• Cabaniols JP, Ravichandran V, Roche PA (Dec 1999). "Phosphorylation of SNAP-23 by the novel kinase SNAK regulates t-SNARE complex assembly". Molecular Biology of the Cell. 10 (12): 4033–41. doi:10.1091/mbc.10.12.4033. PMC 25741. PMID 10588641.
• Li Y, DeFatta R, Anthony C, Sunavala G, De Benedetti A (Feb 2001). "A translationally regulated Tousled kinase phosphorylates histone H3 and confers radioresistance when overexpressed". Oncogene. 20 (6): 726–38. doi:10.1038/sj.onc.1204147. PMID 11314006.
• Silljé HH, Nigg EA (Jul 2001). "Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases". Current Biology. 11 (13): 1068–73. doi:10.1016/S0960-9822(01)00298-6. PMID 11470414.
• Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K (Sep 2003). "Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1". Oncogene. 22 (38): 5927–37. doi:10.1038/sj.onc.1206691. PMID 12955071.
• Norton KS, McClusky D, Sen S, Yu H, Meschonat C, Debenedetti A, Li BD (Jan 2004). "TLK1B is elevated with eIF4E overexpression in breast cancer". The Journal of Surgical Research. 116 (1): 98–103. doi:10.1016/j.jss.2003.08.001. PMID 14732354.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Kodym R, Henöckl C, Fürweger C (Jul 2005). "Identification of the human DEAD-box protein p68 as a substrate of Tlk1". Biochemical and Biophysical Research Communications. 333 (2): 411–7. doi:10.1016/j.bbrc.2005.05.136. PMID 15950181.
• Sunavala-Dossabhoy G, Balakrishnan SK, Sen S, Nuthalapaty S, De Benedetti A (2006). "The radioresistance kinase TLK1B protects the cells by promoting repair of double strand breaks". BMC Molecular Biology. 6: 19. doi:10.1186/1471-2199-6-19. PMC 1242231. PMID 16156902.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Sen SP, De Benedetti A (2006). "TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1". BMC Molecular Biology. 7: 37. doi:10.1186/1471-2199-7-37. PMC 1626478. PMID 17054786.