SNAPC4

snRNA-activating protein complex subunit 4 is a protein that in humans is encoded by the SNAPC4 gene.[5][6]

SNAPC4
Identifiers
Aliases	SNAPC4, PTFalpha, SNAP190, small nuclear RNA activating complex polypeptide 4
External IDs	OMIM: 602777 MGI: 2443935 HomoloGene: 2321 GeneCards: SNAPC4
Gene location (Human) Chr. Chromosome 9 (human)[1] Band 9q34.3 Start 136,375,577 bp[1] End 136,400,168 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 A3 Start 26,362,765 bp[2] End 26,380,653 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • DNA binding • sequence-specific DNA binding • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component • cell nucleus • nucleoplasm • snRNA-activating protein complex Biological process • regulation of transcription, DNA-templated • transcription from RNA polymerase II promoter • transcription, DNA-templated • snRNA transcription from RNA polymerase III promoter • transcription from RNA polymerase III promoter • regulation of transcription from RNA polymerase II promoter • snRNA transcription from RNA polymerase II promoter • cell differentiation • snRNA transcription Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	6621	227644
Ensembl	ENSG00000165684	ENSMUSG00000036281
UniProt	Q5SXM2	Q8BP86
RefSeq (mRNA)	NM_003086	NM_001290419 NM_172339
RefSeq (protein)	NP_003077	NP_001277348 NP_758842
Location (UCSC)	Chr 9: 136.38 – 136.4 Mb	Chr 2: 26.36 – 26.38 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Model organisms

Snapc4 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Salmonella infection	Normal[7]
Citrobacter infection	Normal[8]
All tests and analysis from[9][10]

Model organisms have been used in the study of SNAPC4 function. A conditional knockout mouse line, called Snapc4^{tm1a(KOMP)Wtsi}[11][12] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[13][14][15]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][16] Twenty six tests were carried out on mutant mice and two significant abnormalities were observed. No homozygous mutant embryos were recorded during gestation and, in a separate study, no homozygous animals were observed at weaning. The remaining tests were carried out on adult heterozygous mutant animals and no further abnormalities were observed.[9]

Interactions

SNAPC4 has been shown to interact with SNAPC1,[17] POU2F1[5][18] and SNAPC2.[5][17]

References

1. GRCh38: Ensembl release 89: ENSG00000165684 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000036281 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Wong MW, Henry RW, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N (January 1998). "The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1". Molecular and Cellular Biology. 18 (1): 368–77. doi:10.1128/mcb.18.1.368. PMC 121507. PMID 9418884.
6. "Entrez Gene: SNAPC4 small nuclear RNA activating complex, polypeptide 4, 190kDa".
7. "Salmonella infection data for Snapc4". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Snapc4". Wellcome Trust Sanger Institute.
9. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (June 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
14. Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
15. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
16. van der Weyden L, White JK, Adams DJ, Logan DW (June 2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
17. Ma B, Hernandez N (February 2001). "A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc". The Journal of Biological Chemistry. 276 (7): 5027–35. doi:10.1074/jbc.M009301200. PMID 11056176.
18. Hovde S, Brooks A, Strong K, Geiger JH (March 2002). "Crystallization of the Oct-1/SNAP190 peptide/DNA complex". Acta Crystallographica Section D. 58 (Pt 3): 511–2. doi:10.1107/S0907444901021461. PMID 11856838.

Further reading

• Henry RW, Sadowski CL, Kobayashi R, Hernandez N (April 1995). "A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III". Nature. 374 (6523): 653–6. doi:10.1038/374653a0. PMID 7715707.
• Ma B, Hernandez N (February 2001). "A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc". The Journal of Biological Chemistry. 276 (7): 5027–35. doi:10.1074/jbc.M009301200. PMID 11056176.
• Hovde S, Brooks A, Strong K, Geiger JH (March 2002). "Crystallization of the Oct-1/SNAP190 peptide/DNA complex". Acta Crystallographica Section D. 58 (Pt 3): 511–2. doi:10.1107/S0907444901021461. PMID 11856838.
• Hinkley CS, Hirsch HA, Gu L, LaMere B, Henry RW (May 2003). "The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition". The Journal of Biological Chemistry. 278 (20): 18649–57. doi:10.1074/jbc.M204247200. PMID 12621023.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Gu L, Esselman WJ, Henry RW (July 2005). "Cooperation between small nuclear RNA-activating protein complex (SNAPC) and TATA-box-binding protein antagonizes protein kinase CK2 inhibition of DNA binding by SNAPC". The Journal of Biological Chemistry. 280 (30): 27697–704. doi:10.1074/jbc.M503206200. PMID 15955816.
• Gu L, Husain-Ponnampalam R, Hoffmann-Benning S, Henry RW (September 2007). "The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III". The Journal of Biological Chemistry. 282 (38): 27887–96. doi:10.1074/jbc.M702269200. PMID 17670747.