RIC3

RIC-3 also known as resistance to inhibitors of cholinesterase 3 is a chaperone protein that in humans is encoded by the RIC3 gene. The RIC3 gene was first discovered in C. elegans.[5] RIC-3 protein is conserved in most animals and influences the maturation of various ligand gated ion channels including the serotonin 5-HT₃ receptor and nicotinic acetylcholine receptors,[6][7] particularly the homomeric α₇ nicotinic receptor. RIC-3 enhances currents generated by these receptors by expediting receptor transport to the cell surface and by increasing receptor number.[8]

RIC3
Identifiers
Aliases	RIC3, AYST720, PRO1385, RIC3 acetylcholine receptor chaperone
External IDs	OMIM: 610509 MGI: 2443887 HomoloGene: 49772 GeneCards: RIC3
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p15.4 Start 8,106,056 bp[1] End 8,169,055 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 E3 Start 109,034,312 bp[2] End 109,083,331 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • protein binding involved in protein folding • acetylcholine receptor binding Cellular component • integral component of membrane • neuron projection • Golgi membrane • neuronal cell body • Golgi apparatus • endoplasmic reticulum membrane • intracellular membrane-bounded organelle • endoplasmic reticulum • membrane Biological process • positive regulation of cytosolic calcium ion concentration • synaptic transmission, cholinergic • protein localization to cell surface • protein folding • cellular macromolecular complex assembly Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	79608	320360
Ensembl	ENSG00000166405	ENSMUSG00000048330
UniProt	Q7Z5B4	Q8BPM6
RefSeq (mRNA)	NM_001135109 NM_001206671 NM_001206672 NM_024557 NM_001346690 NM_001346691 NM_001346692 NM_001346693 NM_001346694	NM_001038624 NM_178780 NM_001311161
RefSeq (protein)	NP_001128581 NP_001193600 NP_001193601 NP_001333619 NP_001333620 NP_001333621 NP_001333622 NP_001333623 NP_078833	NP_001033713 NP_001298090 NP_848895
Location (UCSC)	Chr 11: 8.11 – 8.17 Mb	Chr 7: 109.03 – 109.08 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000166405 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000048330 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nguyen M, Alfonso A, Johnson CD, Rand JB (Jun 1995). "Caenorhabditis elegans mutants resistant to inhibitors of acetylcholinesterase". Genetics. 140 (2): 527–35. PMC 1206632. PMID 7498734.
6. Halevi S, Yassin L, Eshel M, Sala F, Sala S, Criado M, Treinin M (Sep 2003). "Conservation within the RIC-3 gene family. Effectors of mammalian nicotinic acetylcholine receptor expression". J Biol Chem. 278 (36): 34411–7. doi:10.1074/jbc.M300170200. PMID 12821669.
7. "Entrez Gene: RIC3 resistance to inhibitors of cholinesterase 3 homolog (C. elegans)".
8. Williams ME, Burton B, Urrutia A, et al. (2005). "Ric-3 promotes functional expression of the nicotinic acetylcholine receptor alpha7 subunit in mammalian cells". J. Biol. Chem. 280 (2): 1257–63. doi:10.1074/jbc.M410039200. PMID 15504725.

Further reading

• Cheng A, Bollan KA, Greenwood SM, et al. (2007). "Differential subcellular localization of RIC-3 isoforms and their role in determining 5-HT3 receptor composition". J. Biol. Chem. 282 (36): 26158–66. doi:10.1074/jbc.M703899200. PMID 17609200.
• Castillo M, Mulet J, Gutiérrez LM, et al. (2007). "Role of the RIC-3 protein in trafficking of serotonin and nicotinic acetylcholine receptors". J. Mol. Neurosci. 30 (1–2): 153–6. doi:10.1385/JMN:30:1:153. PMID 17192664.
• Lansdell SJ, Gee VJ, Harkness PC, et al. (2005). "RIC-3 enhances functional expression of multiple nicotinic acetylcholine receptor subtypes in mammalian cells". Mol. Pharmacol. 68 (5): 1431–8. doi:10.1124/mol.105.017459. PMID 16120769.
• Castillo M, Mulet J, Gutiérrez LM, et al. (2005). "Dual role of the RIC-3 protein in trafficking of serotonin and nicotinic acetylcholine receptors". J. Biol. Chem. 280 (29): 27062–8. doi:10.1074/jbc.M503746200. PMID 15927954.
• Cheng A, McDonald NA, Connolly CN (2005). "Cell surface expression of 5-hydroxytryptamine type 3 receptors is promoted by RIC-3". J. Biol. Chem. 280 (23): 22502–7. doi:10.1074/jbc.M414341200. PMID 15809299.
• Bennett HM, Lees K, et al. (2012). "Xenopus laevis RIC-3 enhances the functional expression of the C. elegans homomeric nicotinic receptor, ACR-16, in Xenopus oocytes". J. Neurochem. 123 (6): 911–8. doi:10.1111/jnc.12013. PMC 3549563. PMID 22970690.
• Koperniak TM, Garg BK, et al. (2013). "Cell-specific effects on surface α7 nicotinic receptor expression revealed by over-expression and knockdown of rat RIC3 protein". J. Neurochem. 124 (3): 300–9. doi:10.1111/jnc.12095. PMID 23157401.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.