Protomer

In structural biology, a protomer is the structural unit of an oligomeric protein. It is the smallest unit composed of at least two different protein chains that form a larger hetero-oligomer by association of two or more copies of this unit.

The term was introduced by Chetverin[1] to make nomenclature in the Na/K-ATPase enzyme unambiguous. This enzyme is composed of two subunits, a large catalytic subunit, alpha and a smaller glycoprotein subunit, beta (plus a proteolipid, called γ-subunit). At the time it was unclear how many of each work together. In addition, when people spoke of a dimer, it was unclear whether they were referring to αβ or to (αβ)2. Chetverin suggested to call αβ a protomer and (αβ)2 a diprotomer.

Protomers usually arrange in cyclic symmetry to form closed point group symmetries.

In chemistry, a so-called protomer is a molecule which displays tautomerism due to position of a proton.[2][3]

Examples

Hemoglobin is a heterotetramer consisting of four subunits (two α and two β). However, structurally and functionally hemoglobin is described better as (αβ)2, so we call it a dimer of two αβ-protomers, that is, a diprotomer.[4]

Aspartate carbamoyltransferase has a α6β6 subunit composition. The six αβ-protomers are arranged in D3 symmetry.

Viral capsid are often composed of protomers.

Examples in chemistry include tyrosine and 4-aminobenzoic acid. The former may be deprotonated to form the carboxylate and phenoxide anions,[5] and the later may be protonated at the amino or carboxyl groups.[6]

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References
  1. Chetverin, A.B. (1986). "Evidence for a diprotomeric structure of Na, K-ATPase: Accurate determination of protein concentration and quantitative end-group analysis". FEBS Lett. 196: 121–125. doi:10.1016/0014-5793(86)80225-3. PMID 3002859.
  2. P. M. Lalli, B. A. Iglesias, H. E. Toma, G. F. de Sa, R. J. Daroda, J. C. Silva Filho, J. E. Szulejko, K. Araki and M. N. Eberlin, J. Mass Spectrom., 2012, 47, 712–719.
  3. C. Lapthorn, T. J. Dines, B. Z. Chowdhry, G. L. Perkins and F. S. Pullen, Rapid Commun. Mass Spectrom., 2013, 27, 2399–2410.
  4. Buxbaum, E. (2007). Fundamentals of protein structure and function. New York: Springer. pp. 105–120. ISBN 978-0-387-26352-6.
  5. J. Am. Chem. Soc., 2009, 131 (3), pp 1174–1181
  6. J. Phys. Chem. A, 2011, 115 (26), pp 7625–7632


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