Nitroreductase

Nitroreductases are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds, including those containing the nitro functional group. Members of this family utilise flavin mononucleotide as a cofactor and are often found to be homodimers.[1][2]

Nitroreductase
Identifiers
Symbol	Nitroreductase
Pfam	PF00881
InterPro	IPR000415
CDD	cd02062
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary PDB PDB: 1bkj​ PDB: 1ds7​ PDB: 1f5v​ PDB: 1icr​ PDB: 1icu​ PDB: 1icv​ PDB: 1idt​ PDB: 1kqb​ PDB: 1kqc​ PDB: 1kqd​

Members of this family include oxygen-insensitive NAD(P)H nitroreductase (flavin mononucleotide-dependent nitroreductase) (6,7-dihydropteridine reductase) (EC 1.5.1.34) and NADH dehydrogenase (EC 1.6.99.3). A number of these proteins are described as oxidoreductases. They are primarily found in bacterial lineages though a number of eukaryotic homologs have been identified: C. elegans P34273, D. melanogaster Q8T3Q0, Q9VTE7, mouse Q9DCX8 and human O75989. This protein is not found in photosynthetic eukaryotes. The sequences containing this entry in photosynthetic organisms are possible false positives.

The nitroreductase of Enterobacter cloacae was identified by Bryant and Deluca[3] in a strain isolated from a munitions facility, on the basis of its ability to metabolize TNT (trinitrotoluene). Since then many homologues have been identified and the family is now known to include members in diverse organisms, that catalize diverse reactions. The iodotyrosine deiodenase of mammals is a dehalogenase, the BluB of Sinorhizobium meliloti cannibalizes the bound flavin mononucleotideto furnish a critical intermediate in vitamin B12 biosynthesis.

Crystal structures of the E. cloacae and E. coli enzymes have been published with a variety of substrates and analogues bound.

An example of a potential cold-active enzyme for prodrug therapy was described using a cold-active nitroreductase, Ssap-NtrB[4] (Çelik and Yetis, 2012). Despite Ssap-NtrB derived from a mesophilic bacterium, it showed optimal activity at 20°C against cancer prodrugs. Authors comment that the cold-activity of this novel enzyme will be useful for therapies in combination with crymotherapy, exposing the target tissue to low temperatures in order to trigger the enzyme activity to activate the drug only where is required. Moreover, the enzyme could also be used for bioremediation of compounds of explosive and volatile nature in regions where high activity at low temperatures is needed.

Subfamilies

• Cob(II)yrinic acid a,c-diamide reductase InterPro: IPR012825

Human proteins containing this domain

IYD;

References

1. Hecht HJ, Erdmann H, Park HJ, Sprinzl M, Schmid RD (December 1995). "Crystal structure of NADH oxidase from Thermus thermophilus". Nat. Struct. Biol. 2 (12): 1109–14. doi:10.1038/nsb1295-1109. PMID 8846223.
2. de Oliveira IM, Henriques JA, Bonatto D (April 2007). "In silico identification of a new group of specific bacterial and fungal nitroreductases-like proteins". Biochem. Biophys. Res. Commun. 355 (4): 919–25. doi:10.1016/j.bbrc.2007.02.049. PMID 17331467.
3. Bryant, C.; DeLuca, M. (1991-03-05). "Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae". Journal of Biological Chemistry. 266 (7): 4119–4125. ISSN 0021-9258. PMID 1999405.
4. Çelik, Ayhan; Yetiş, Gülden (2012-06-01). "An unusually cold active nitroreductase for prodrug activations". Bioorganic & Medicinal Chemistry. 20 (11): 3540–3550. doi:10.1016/j.bmc.2012.04.004. PMID 22546205.

This article incorporates text from the public domain Pfam and InterPro: IPR000415