Musashi-1

RNA-binding protein Musashi homolog 1 also known as Musashi-1 is a protein that in humans is encoded by the MSI1 gene.[5][6]

MSI1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1UAW, 2MSS, 2MST, 2RS2
Identifiers
Aliases	MSI1, musashi RNA binding protein 1
External IDs	OMIM: 603328 MGI: 107376 HomoloGene: 55657 GeneCards: MSI1
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q24.31 Start 120,341,330 bp[1] End 120,369,164 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 F Start 115,429,599 bp[2] End 115,455,698 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • single-stranded RNA binding • nucleic acid binding • poly(U) RNA binding • identical protein binding • RNA binding Cellular component • cytoplasm • polysome • cell nucleus • ribonucleoprotein Biological process • nervous system development Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4440	17690
Ensembl	ENSG00000135097	ENSMUSG00000054256
UniProt	O43347	Q61474
RefSeq (mRNA)	NM_002442	NM_008629 NM_001376960 NM_001376961 NM_001376962 NM_001376963
RefSeq (protein)	NP_002433	NP_032655 NP_001363889 NP_001363890 NP_001363891 NP_001363892
Location (UCSC)	Chr 12: 120.34 – 120.37 Mb	Chr 5: 115.43 – 115.46 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a protein containing two conserved tandem RNA recognition motifs and functions as an RNA binding protein that is involved in post-transcriptional gene editing. It is a stem cell marker that controls the balance between self-renewal and terminal differentiation.[7][6]

Clinical significance

Over expression of this gene is associated with the grade of the malignancy and proliferative activity in gliomas and melanomas.[7] An increased expression of MSI1 protein is observed in endometriosis and endometrial carcinoma [8] siRNA-mediated inhibition of MSI expression in endometrial carcinoma cells induces apoptosis and inhibits cell proliferation by affecting the Notch signaling pathway [9]

MSI1 is highly expressed in neural progenitor cells and is required for normal development of the brain. A mutation in these gene is responsible for autosomal recessive primary microcephaly. MSI1 also interacts with the Zika virus genome and may explain why these cells are highly susceptible to Zika virus infection.[10][11]

See also

• Musashi-2

References

1. GRCh38: Ensembl release 89: ENSG00000135097 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000054256 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Good P, Yoda A, Sakakibara S, Yamamoto A, Imai T, Sawa H, Ikeuchi T, Tsuji S, Satoh H, Okano H (September 1998). "The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells". Genomics. 52 (3): 382–4. doi:10.1006/geno.1998.5456. PMID 9790759.
6. "Entrez Gene: MSI1 musashi homolog 1 (Drosophila)".
7. Glazer RI, Vo DT, Penalva LO (2012). "Musashi1: an RBP with versatile functions in normal and cancer stem cells". Frontiers in Bioscience. 17: 54–64. doi:10.2741/3915. PMID 22201732.
8. Götte, M; Wolf, M; Staebler, A; Buchweitz, O; Kelsch, R; Schüring, AN; Kiesel, L (2008). "Increased expression of the adult stem cell marker Musashi-1 in endometriosis and endometrial carcinoma". Journal of Pathology. 215 (3): 317–29. doi:10.1002/path.2364. PMID 18473332.
9. Götte, M; Greve, B; Kelsch, R; Müller-Uthoff, H; Weiss, K; Kharabi Masouleh, B; Sibrowski, W; Kiesel, L; Buchweitz, O (2011). "The adult stem cell marker Musashi-1 modulates endometrial carcinoma cell cycle progression and apoptosis via Notch-1 and p21WAF1/CIP1". International Journal of Cancer. 129 (8): 2042–2049. doi:10.1002/ijc.25856. PMID 21165952.
10. Chavali PL, Stojic L, Meredith LW, Joseph N, Nahorski MS, Sanford TJ, Sweeney TR, Krishna BA, Hosmillo M, Firth AE, Bayliss R, Marcelis CL, Lindsay S, Goodfellow I, Woods CG, Gergely F (2017). "Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication". Science. 357 (6346): 83–88. doi:10.1126/science.aam9243. PMC 5798584. PMID 28572454.
11. Griffin DE (2017). "Why are neurons susceptible to Zika virus?". Science. 357 (6346): 33–34. doi:10.1126/science.aan8626. PMID 28684491.

Further reading

• Okano H, Imai T, Okabe M (April 2002). "Musashi: a translational regulator of cell fate". Journal of Cell Science. 115 (Pt 7): 1355–9. PMID 11896183.
• Toda M, Iizuka Y, Yu W, Imai T, Ikeda E, Yoshida K, Kawase T, Kawakami Y, Okano H, Uyemura K (April 2001). "Expression of the neural RNA-binding protein Musashi1 in human gliomas". Glia. 34 (1): 1–7. doi:10.1002/glia.1034. PMID 11284014.
• Imai T, Tokunaga A, Yoshida T, Hashimoto M, Mikoshiba K, Weinmaster G, Nakafuku M, Okano H (June 2001). "The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA". Molecular and Cellular Biology. 21 (12): 3888–900. doi:10.1128/MCB.21.12.3888-3900.2001. PMC 87052. PMID 11359897.
• Sakakibara S, Nakamura Y, Satoh H, Okano H (October 2001). "Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS". The Journal of Neuroscience. 21 (20): 8091–107. doi:10.1523/JNEUROSCI.21-20-08091.2001. PMID 11588182.
• Kanemura Y, Mori K, Sakakibara S, Fujikawa H, Hayashi H, Nakano A, Matsumoto T, Tamura K, Imai T, Ohnishi T, Fushiki S, Nakamura Y, Yamasaki M, Okano H, Arita N (September 2001). "Musashi1, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity". Differentiation; Research in Biological Diversity. 68 (2–3): 141–52. doi:10.1046/j.1432-0436.2001.680208.x. PMID 11686236.
• Kanemura Y, Sakakibara S, Okano H (2002). "Identification of Musashi1-positive cells in human normal and neoplastic neuroepithelial tissues by immunohistochemical methods". Methods in Molecular Biology. 198: 273–81. doi:10.1385/1-59259-186-8:273. ISBN 1-59259-186-8. PMID 11951628.
• Shu HJ, Saito T, Watanabe H, Ito JI, Takeda H, Okano H, Kawata S (April 2002). "Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines". Biochemical and Biophysical Research Communications. 293 (1): 150–4. doi:10.1016/S0006-291X(02)00175-4. PMID 12054577.
• Miyanoiri Y, Kobayashi H, Imai T, Watanabe M, Nagata T, Uesugi S, Okano H, Katahira M (October 2003). "Origin of higher affinity to RNA of the N-terminal RNA-binding domain than that of the C-terminal one of a mouse neural protein, musashi1, as revealed by comparison of their structures, modes of interaction, surface electrostatic potentials, and backbone dynamics". The Journal of Biological Chemistry. 278 (42): 41309–15. doi:10.1074/jbc.M306210200. PMID 12907678.
• Nishimura S, Wakabayashi N, Toyoda K, Kashima K, Mitsufuji S (August 2003). "Expression of Musashi-1 in human normal colon crypt cells: a possible stem cell marker of human colon epithelium". Digestive Diseases and Sciences. 48 (8): 1523–9. doi:10.1023/A:1024763723240. PMID 12924647.
• Lovell MA, Markesbery WR (August 2005). "Ectopic expression of Musashi-1 in Alzheimer disease and Pick disease". Journal of Neuropathology and Experimental Neurology. 64 (8): 675–80. doi:10.1097/01.jnen.0000173891.17176.5b. PMID 16106215.
• Battelli C, Nikopoulos GN, Mitchell JG, Verdi JM (January 2006). "The RNA-binding protein Musashi-1 regulates neural development through the translational repression of p21WAF-1". Molecular and Cellular Neurosciences. 31 (1): 85–96. doi:10.1016/j.mcn.2005.09.003. PMID 16214366.
• Chan C, Moore BE, Cotman CW, Okano H, Tavares R, Hovanesian V, Pinar H, Johanson CE, Svendsen CN, Stopa EG (October 2006). "Musashi1 antigen expression in human fetal germinal matrix development". Experimental Neurology. 201 (2): 515–8. doi:10.1016/j.expneurol.2006.04.023. PMID 16777095.