NCI-60
The NCI-60 cancer cell line panel is a group of 60 human cancer cell lines used by the National Cancer Institute (NCI) for the screening of compounds to detect potential anticancer activity.[1]
Purpose
The screening procedure is called the NCI-60 Human Tumor Cell Lines Screen, and it is one of the Discovery & Development Services of NCI's Developmental Therapeutics Program (DTP).[2][3][4] The screening rates for each cell line the cytostatic and cytotoxic impact of tested substances.[2]
Due to the diversity of the cell lines, it is possible to compare tested compounds by their effect patterns, high correlation potentially corresponding to similar effect mechanisms.[2] An automated comparison against a database of more than 88,000 pure compounds and more than 34,000 crude extracts (as of 6 January 2017) is provided by the COMPARE tool, which shows a list of substances ranked by the Pearson correlation coefficients for a given test substance.[5]
The same panel is used in the Molecular Target Program for the characterization of molecular targets. Measurements include protein levels, RNA measurements, mutation status and enzyme activity levels.[6]
Cell Lines
The panel holds cell lines representing leukemia, melanoma, non-small-cell lung carcinoma, and cancers of the brain, ovary, breast, colon, kidney, and prostate.[1][2]
13 additional cell lines are evaluated for use in the screening program, among them two lines deriving from so far not represented small-cell lung carcinoma.[1]
All cell lines but one (as of 6 January 2017) are available to other laboratories, including the additional cell lines.[1]
Misidentification and Misclassification
Starting around the turn of the millennium, several cell lines were found to be misidentified or misclassified at the time of investigation or earlier:[7][8][9]
MDA-MB-435, originally classified as breast cancer cell line, was identified as being a melanoma cell line.[7] When investigated further, current MDA-MB-435 appeared to be derived from the same individual as cell line M14.[7] An examination of clones made clear that the mixup with M14 happened very early in the history of MDA-MB-435, before establishment in the Developmental Therapeutics Program.[7]
NCI/ADR-RES, originally classified as breast cancer cell line, was identified as being an ovarian tumor cell line.[8] NCI/ADR-RES appears to have been derived at some point in time from cell line OVCAR-8.[8] Originally the cell line was named MCF-7/ADR-RES; it was renamed together with the change in classification.[8]
Two brain cancer cell lines, SNB-19 and U251, were discovered to come from the same person.[9] This makes a mixup likely.
A 61st cell line, MDA-N, has been confirmed to being derived from the misclassified MDA-MB-435 cell line.[7] So it is also misclassified, really being a melanoma cell line, but as of 6 January 2018 the official website still lists it as breast cancer cell line.[1] This cell line is not available as of 6 January 2018.[1]
The International Cell Line Authentication Committee maintains a list of contaminated cell lines.[10] It includes the cell lines reported above, although usually with reporting dates and articles considerably later than the first dates and articles rising specific concern regarding the respective cell line.[10][7][8][9]
Continued Use of Wrong Classification
As of 2014 Hundreds of research studies still use MDA-MB-435 as model of breast cancer, even after it was officially declared to be a melanoma cell line in 2007, and even in highest-rated international peer-reviewed journals.[11] Similar is true for other misidentified cell lines.[11]
List of NCI-60 Cell Lines
| Cell line | Tumor classification | (Potentially) misidentified | Original classification | Unavailable | Doubling Time (Hours) | Inoculation Density | Cellosaurus identifier and link |
|---|---|---|---|---|---|---|---|
| CCRF-CEM | Leukemia | 26.7 | 40000 | CCRF-CEM (RRID:CVCL_0207) | |||
| HL-60(TB) | Leukemia | 28.6 | 40000 | HL-60(TB) (RRID:CVCL_A794) | |||
| K-562 | Leukemia | 19.6 | 5000 | K-562 (RRID:CVCL_0004) | |||
| MOLT-4 | Leukemia | 27.9 | 30000 | MOLT-4 (RRID:CVCL_0013) | |||
| RPMI-8226 | Leukemia | 33.5 | 20000 | RPMI-8226 (RRID:CVCL_0014) | |||
| SR | Leukemia | 28.7 | 20000 | SR (RRID:CVCL_1711) | |||
| A549/ATCC | Non-Small Cell Lung | 22.9 | 7500 | A-549 (RRID:CVCL_0023) | |||
| EKVX | Non-Small Cell Lung | 43.6 | 20000 | EKVX (RRID:CVCL_1195) | |||
| HOP-62 | Non-Small Cell Lung | 39 | 10000 | HOP-62 (RRID:CVCL_1285) | |||
| HOP-92 | Non-Small Cell Lung | 79.5 | 20000 | HOP-92 (RRID:CVCL_1286) | |||
| NCI-H226 | Non-Small Cell Lung | 61 | 20000 | NCI-H226 (RRID:CVCL_1544) | |||
| NCI-H23 | Non-Small Cell Lung | 33.4 | 20000 | NCI-H23 (RRID:CVCL_1547) | |||
| NCI-H322M | Non-Small Cell Lung | 35.3 | 20000 | NCI-H322M (RRID:CVCL_1557) | |||
| NCI-H460 | Non-Small Cell Lung | 17.8 | 7500 | NCI-H460 (RRID:CVCL_0459) | |||
| NCI-H522 | Non-Small Cell Lung | 38.2 | 20000 | NCI-H522 (RRID:CVCL_1567) | |||
| COLO 205 | Colon | 23.8 | 15000 | COLO 205 (RRID:CVCL_0218) | |||
| HCC-2998 | Colon | 31.5 | 15000 | HCC2998 (RRID:CVCL_1266) | |||
| HCT116 | Colon | 17.4 | 5000 | HCT 116 (RRID:CVCL_0291) | |||
| HCT-15 | Colon | 20.6 | 10000 | HCT 15 (RRID:CVCL_0292) | |||
| HT-29 | Colon | 19.5 | 5000 | HT-29 (RRID:CVCL_0320) | |||
| KM12 | Colon | 23.7 | 15000 | KM12 (RRID:CVCL_1331) | |||
| SW-620 | Colon | 20.4 | 10000 | SW620 (RRID:CVCL_0547) | |||
| SF-268 | CNS | 33.1 | 15000 | SF268 (RRID:CVCL_1689) | |||
| SF-295 | CNS | 29.5 | 10000 | SF295 (RRID:CVCL_1690) | |||
| SF-539 | CNS | 35.4 | 15000 | SF539 (RRID:CVCL_1691) | |||
| SNB-19 | CNS | yes | CNS | 34.6 | 15000 | SNB-19 (RRID:CVCL_0535) | |
| SNB-75 | CNS | 62.8 | 20000 | SNB-75 (RRID:CVCL_1706) | |||
| U251 | CNS | yes | CNS | 23.8 | 7500 | U-251MG (RRID:CVCL_0021) | |
| LOX IMVI | Melanoma | 20.5 | 7500 | LOX-IMVI (RRID:CVCL_1381) | |||
| MALME-3M | Melanoma | 46.2 | 20000 | Malme-3M (RRID:CVCL_1438) | |||
| M14 | Melanoma | 26.3 | 15000 | M14 (RRID:CVCL_1395) | |||
| MDA-MB-435 | Melanoma | yes | Breast | 25.8 | 15000 | MDA-MB-435 (RRID:CVCL_0417) | |
| SK-MEL-2 | Melanoma | 45.5 | 20000 | SK-MEL-2 (RRID:CVCL_0069) | |||
| SK-MEL-28 | Melanoma | 35.1 | 10000 | SK-MEL-28 (RRID:CVCL_0526) | |||
| SK-MEL-5 | Melanoma | 25.2 | 10000 | SK-MEL-5 (RRID:CVCL_0527) | |||
| UACC-257 | Melanoma | 38.5 | 20000 | UACC-257 (RRID:CVCL_1779) | |||
| UACC-62 | Melanoma | 31.3 | 10000 | UACC-62 (RRID:CVCL_1780) | |||
| IGR-OV1 | Ovarian | 31 | 10000 | IGROV-1 (RRID:CVCL_1304) | |||
| OVCAR-3 | Ovarian | 34.7 | 10000 | OVCAR-3 (RRID:CVCL_0465) | |||
| OVCAR-4 | Ovarian | 41.4 | 15000 | OVCAR-4 (RRID:CVCL_1627) | |||
| OVCAR-5 | Ovarian | 48.8 | 20000 | OVCAR-5 (RRID:CVCL_1628) | |||
| OVCAR-8 | Ovarian | 26.1 | 10000 | OVCAR-8 (RRID:CVCL_1629) | |||
| NCI/ADR-RES (originally MCF-7/ADR-RES) | Ovarian | yes | Breast | 34 | 15000 | NCI-ADR-RES (RRID:CVCL_1452) | |
| SK-OV-3 | Ovarian | 48.7 | 20000 | SK-OV-3 (RRID:CVCL_0532) | |||
| 786-O | Renal | 22.4 | 10000 | 786-O (RRID:CVCL_1051) | |||
| A498 | Renal | 66.8 | 25000 | A-498 (RRID:CVCL_1056) | |||
| ACHN | Renal | 27.5 | 10000 | ACHN (RRID:CVCL_1067) | |||
| CAKI-1 | Renal | 39 | 10000 | Caki-1 (RRID:CVCL_0234) | |||
| RXF 393 | Renal | 62.9 | 15000 | RXF 393L (RRID:CVCL_1673) | |||
| SN12C | Renal | 29.5 | 15000 | SN12C (RRID:CVCL_1705) | |||
| TK-10 | Renal | 51.3 | 15000 | TK-10 (RRID:CVCL_1773) | |||
| UO-31 | Renal | 41.7 | 15000 | UO-31 (RRID:CVCL_1911) | |||
| PC-3 | Prostate | 27.1 | 7500 | PC-3 (RRID:CVCL_0035) | |||
| DU-145 | Prostate | 32.3 | 10000 | DU145 (RRID:CVCL_0105) | |||
| MCF7 | Breast | 25.4 | 10000 | MCF-7 (RRID:CVCL_0031) | |||
| MDA-MB-231/ATCC | Breast | 41.9 | 20000 | MDA-MB-231 (RRID:CVCL_0062) | |||
| MDA-MB-468 | Breast | 62 | 2000 | MDA-MB-468 (RRID:CVCL_0419) | |||
| HS 578T | Breast | 53.8 | 20000 | Hs 578T (RRID:CVCL_0332) | |||
| MDA-N | Melanoma | yes | Breast | unavailable | 22.5 | 15000 | MDA-N (RRID:CVCL_1910) |
| BT-549 | Breast | 53.9 | 20000 | BT-549 (RRID:CVCL_1092) | |||
| T-47D | Breast | 45.5 | 20000 | T-47D (RRID:CVCL_0553) |
List of Additional Cell Lines
| Cell line | Tumor classification | Species | Inoculation Density | Cellosaurus identifier and link |
|---|---|---|---|---|
| LXFL 529 | Non-Small Cell Lung | human | 10000 | LXFL 529L (RRID:CVCL_D085) |
| DMS 114 | Small Cell Lung | human | 20000 | DMS 114 (RRID:CVCL_1174) |
| SHP-77 | Small Cell Lung | human | 40000 | SHP-77 (RRID:CVCL_1693) |
| DLD-1 | Colon | human | 50000 | DLD-1 (RRID:CVCL_0248) |
| KM20L2 | Colon | human | 10000 | KM20L2 (RRID:CVCL_D889) |
| SNB-78 | CNS | human | 20000 | SNB-78 (RRID:CVCL_B321) |
| XF 498 | CNS | human | 20000 | XF 498 (RRID:CVCL_8928) |
| RPMI-7951 | Melanoma | human | 20000 | RPMI-7951 (RRID:CVCL_1666) |
| M19-MEL | Melanoma | human | 10000 | M19-MEL (RRID:CVCL_B415) |
| RXF-631 | Renal | human | 10000 | RXF 631L (RRID:CVCL_A780) |
| SN12K1 | Renal | human | 10000 | SN12K1 (RRID:CVCL_D106) |
| P388 | Leukemia | mouse | 50000 | P388 (RRID:CVCL_7222) |
| P388/ADR | Leukemia | mouse | 50000 | P388/ADR (RRID:CVCL_IZ75) |
References
- "Cell Lines in the In Vitro Screen". Developmental Therapeutics Program. National Cancer Institute. 8 May 2015. Retrieved 6 January 2018.
- "NCI-60 Human Tumor Cell Lines Screen". Developmental Therapeutics Program. National Cancer Institute. 26 August 2015. Retrieved 6 January 2018.
- "Discovery & Development Services". Developmental Therapeutics Program. National Cancer Institute. 26 August 2015. Retrieved 6 January 2018.
- "Welcome to the Developmental Therapeutics Program". Developmental Therapeutics Program. National Cancer Institute. Retrieved 6 January 2018.
- "COMPARE Analysis". Developmental Therapeutics Program. National Cancer Institute. 11 August 2015. Retrieved 6 January 2018.
- "Molecular Targets". Developmental Therapeutics Program. National Cancer Institute. 12 May 2015. Retrieved 7 January 2018.
- "MDA-MB-435, and its derivation MDA-N, are Melanoma cell lines, not breast cancer cell lines". Developmental Therapeutics Program. National Cancer Institute. 8 May 2015. Retrieved 6 January 2018.
- "Cell Line NCI/ADR-RES is an Ovarian tumor cell line, not a Breast line". Developmental Therapeutics Program. National Cancer Institute. 8 May 2015. Retrieved 6 January 2018.
- "CNS cell lines SNB-19 and U251 are derived from the same individual". Developmental Therapeutics Program. National Cancer Institute. 8 May 2015. Retrieved 6 January 2018.
- Database of Cross-Contaminated or Misidentified Cell Lines (PDF), 8.0, International Cell Line Authentication Committee (ICLAC), 1 December 2016, retrieved 6 January 2018
- Prasad, Vidudala VTS; Gopalan, Ramprasad OG (2 June 2015). "Continued use of MDA-MB-435, a melanoma cell line, as a model for human breast cancer, even in year, 2014". NPJ Breast Cancer. 1: 15002–. doi:10.1038/npjbcancer.2015.2. PMC 5515196. PMID 28721362.
- "Cellosaurus search result: 61 hits for "NCI60 cancer cell line panel"". Cellosaurus. Swiss Institute of Bioinformatics. Retrieved 7 January 2018. The reference includes the list of linked pages in the search result.
- "Cellosaurus - a knowledge resource on cell lines". Cellosaurus. Swiss Institute of Bioinformatics. Retrieved 17 January 2018. The reference includes the pages found by searching with the respective cell line names as given in the List of Additional Cell Lines.