Glutamate rich 5

Glutamate Rich Protein 5 is a protein in humans encoded by the ERICH5 gene, also known as Chromosome 8 open reading frame 47 (C8orf47).

ERICH5
Identifiers
AliasesERICH5, C8orf47, glutamate rich 5
External IDsMGI: 2447772 HomoloGene: 52129 GeneCards: ERICH5
Gene location (Human)
Chr.Chromosome 8 (human)[1]
Band8q22.2Start98,064,522 bp[1]
End98,093,610 bp[1]
Orthologs
SpeciesHumanMouse
Entrez

203111

239368

Ensembl

ENSG00000177459

ENSMUSG00000044726

UniProt

Q6P6B1

Q8K0S2

RefSeq (mRNA)

NM_173549
NM_001170806

NM_173421

RefSeq (protein)

NP_001164277
NP_775820

NP_775597

Location (UCSC)Chr 8: 98.06 – 98.09 MbChr 15: 34.45 – 34.47 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Gene

The ERICH5 gene is located on human chromosome 8 at 8q22.2 and spans 29 kb on the plus strand of the DNA. [5] ERICH5 contains three exons and two introns. [6] ERICH5 is also known as C8orf47 and Glutamate Rich 5.[5]

Protein

Isoforms

The ERICH5 protein has two isofroms. The longest isofrom, isofrom 1, spans 1,550 base pairs and is composed of 374 amino acids. The second isoform lacks the third and final exon and is 596 base pairs long.[6]

Domains and motifs

ERICH5 contains one conserved domain, a domain of unknown function called DUF4573.[7] ERICH5 is predicted to contain two highly conserved motifs, an APC/C binding motif and the LIG_FHA_2 motif.[8] The APC/C motif spans amino acid 222-226 and serves as a binding site for the Anaphase- promoting complex.[9] The LIG_FHA_2 motif is involved in the cell checkpoint pathway and is found in many proteins localized in the nucleus that regulate cell cycle.[10]

Post-translational modifications

ERICH5 is predicted to undergo several post translational modifications including phosphorylation, O-glycosylation, and Sumolyation. [11][12] [13]Many of the phosphorylation sites and O-glycosylation sites were predicted at the same amino acid. The post translational modifications shown are those conserved among ERICH5 orthologs. Several kinases were predicted to phosphorylate ERICH5 including PKC, cdc2, CKI, PKA, DNAPK, ATM, EGFR, and CKII.[11]

Post Translational Modification Amino Acid Location
Phosphorylation S4, S5, S27, S32, S49, T50, S58, S100, T101, T104, T138, S169, S227, S234, T239,T248, S274, T289, S307,Y320, T340, T346
O-Glycosylation S5,S27, S32, S49, T50, S58, S100, T101, T104, T138, S169, S227, S234, T239, T248, S274, T289,S307
Sumolyation K121, K131, K211, K251, ,K343, K360

Secondary structure

ERICH5 was predicted to contain three alpha helices and two beta sheets as well as regions of random coils. [14]

ERICH5 Secondary Structure. [14]
ERICH5 protein interactions. [15]

Sub-cellular localization

ERICH5 was predicted to be localized in the nucleus.[16]

Protein interactions

ERICH5 was predicted to interact with several proteins through yeast two hybrid screening and affinity chromatography.[15] Several of the proteins ERICH5 was predicted to interact with were also localized in the nucleus.[15]

Expression
Normal expression of ERICH5 in various tissues.[17]

Normal expression

ERICH5 shows elevated levels of expression in the fetal liver, liver, pancreas, and retina compared to other tissues.

Expression in disease
Expression of ERICH5 in Alcoholic Hepatitis. [18]

ERICH5 shows increased expression in Alcoholic Hepatitis.

Homology

Paralogs

There are no known paralogs of ERICH5. [19]

Orthologs

True orthologs of ERICH5 have only been identified among mammalian species. The most distantly related mammalian ortholog is the Monodelphis domestica or the Gray Short Tailed Opossum.[20]

Species Name Common Names MYA % Similarity % Identity Accession # Protein Length
Acinonyx jubatus Cheetah 96 71% 65% XP_014937513.1 358
Cerathotherium White Rhino 96 71% 66% XP_004431343.2 358
Trichechus manatus latirostris Manatee 105 68% 59% XP_004370879.3 342
Monodelphis Gray Short tailed opossum 159 40% 28% XP_007488209.1 478

Distant orthologs

ERICH5 has distant orthologs among birds and reptiles. These distant orthologs contain only the third exon of ERICH5.[21]

Species Name Common Name MYA % Similarity % Identity Accession # Protein Length
Gallus gallus Chicken 312 80% 64% XP_004940017.1 207
Chysemys picta belli Painted Turtle 312 74% 59% XP_008166572.1 442
gollark: Oh, I deleted them so your computers won't work, mwahahaha.
gollark: IT HAS A HELP COMMAND
gollark: It's ++remind.
gollark: ++delete MOSFET
gollark: Blame.. .whoever compiles the fortune-mod database?

References
  1. GRCh38: Ensembl release 89: ENSG00000177459 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000044726 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Database, GeneCards Human Gene. "ERICH5 Gene - GeneCards | ERIC5 Protein | ERIC5 Antibody". www.genecards.org. Retrieved 2018-05-01.
  6. "ERICH5 glutamate rich 5 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.
  7. "NCBI Conserved Domain Search". www.ncbi.nlm.nih.gov. Retrieved 2018-05-07.
  8. "ELM - unknown". elm.eu.org. Retrieved 2018-05-07.
  9. "ELM - Detail for DEG_APCC_KENBOX_2". elm.eu.org. Retrieved 2018-05-07.
  10. "ELM - Detail for LIG_FHA_2". elm.eu.org. Retrieved 2018-05-07.
  11. "NetPhos 3.1 Server - prediction results". www.cbs.dtu.dk. Retrieved 2018-05-07.
  12. "NetOGlyc 4.0 Server - prediction results". www.cbs.dtu.dk. Retrieved 2018-05-07.
  13. "GPS-SUMO: Prediction of SUMOylation Sites & SUMO-interaction Motifs". sumosp.biocuckoo.org. Retrieved 2018-05-07.
  14. "Result for job ERICH5". raptorx.uchicago.edu. Retrieved 2018-05-07.
  15. "PSICQUIC View". EMBL-EBI.
  16. "PSORTII". PSORTII.
  17. Dezso, Z (2008). "A comprehensive functional analysis of tissue specificity of human gene expression". BMC Biology. 6: 49. doi:10.1186/1741-7007-6-49. PMC 2645369. PMID 19014478.
  18. "Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis".
  19. "Gene: ERICH5 (ENSG00000177459) - Comparative Genomics - Homo sapiens - Ensembl genome browser 92". useast.ensembl.org. Retrieved 2018-05-07.
  20. "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2018-05-07.
  21. "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2018-05-07.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.