CNOT8

CCR4-NOT transcription complex subunit 8 is a protein that in humans is encoded by the CNOT8 gene.[5][6][7] It is a subunit of the CCR4-Not deadenylase complex.

CNOT8
Identifiers
Aliases	CNOT8, CAF1, CALIF, Caf1b, POP2, hCAF1, CCR4-NOT transcription complex subunit 8
External IDs	OMIM: 603731 MGI: 1916375 HomoloGene: 48304 GeneCards: CNOT8
Gene location (Human) Chr. Chromosome 5 (human)[1] Band 5q33.2 Start 154,857,553 bp[1] End 154,876,793 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11|11 B1.3 Start 58,104,153 bp[2] End 58,118,594 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • poly(A)-specific ribonuclease activity • 3'-5'-exoribonuclease activity • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • metal ion binding • GO:0001948 protein binding • RNA binding • nucleic acid binding • nuclease activity • exonuclease activity • hydrolase activity Cellular component • cytoplasm • cytosol • CCR4-NOT complex • intracellular • cell nucleus • P-body • CCR4-NOT core complex Biological process • regulation of transcription, DNA-templated • nucleic acid phosphodiester bond hydrolysis • positive regulation of mRNA catabolic process • transcription, DNA-templated • gene silencing by miRNA • positive regulation of cell proliferation • exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay • gene silencing by RNA • regulation of translation • negative regulation of cell proliferation • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest • RNA phosphodiester bond hydrolysis, exonucleolytic • nuclear-transcribed mRNA poly(A) tail shortening • negative regulation of translation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9337	69125
Ensembl	ENSG00000155508	ENSMUSG00000020515
UniProt	Q9UFF9	Q9D8X5
RefSeq (mRNA)	NM_001301073 NM_001301074 NM_001301075 NM_001301077 NM_001301080 NM_001301082 NM_001301083 NM_001301086 NM_004779	NM_026949 NM_001356477 NM_001363084 NM_001363085 NM_001363086
RefSeq (protein)	NP_001288002 NP_001288003 NP_001288004 NP_001288006 NP_001288009 NP_001288011 NP_001288012 NP_001288015 NP_004770	NP_081225 NP_001343406 NP_001350013 NP_001350014 NP_001350015
Location (UCSC)	Chr 5: 154.86 – 154.88 Mb	Chr 11: 58.1 – 58.12 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

CNOT8 has been shown to interact with BTG2,[8] BTG1,[8][9] CNOT1[6][10] and CNOT3.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000155508 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020515 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Fidler C, Wainscoat JS, Boultwood J (May 1999). "The human POP2 gene: identification, sequencing, and mapping to the critical region of the 5q- syndrome". Genomics. 56 (1): 134–6. doi:10.1006/geno.1998.5687. PMID 10036195.
6. Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (March 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Res. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.
7. "Entrez Gene: CNOT8 CCR4-NOT transcription complex, subunit 8".
8. Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L (March 2001). "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway". J. Biol. Chem. 276 (13): 9640–8. doi:10.1074/jbc.M008201200. PMID 11136725.
9. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514.
10. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

Further reading

• Bogdan JA, Adams-Burton C, Pedicord DL, Sukovich DA, Benfield PA, Corjay MH, Stoltenborg JK, Dicker IB (1998). "Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1". Biochem. J. 336 (Pt 2): 471–81. doi:10.1042/bj3360471. PMC 1219893. PMID 9820826.
• Hartley JL, Temple GF, Brasch MA (2000). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L (2001). "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway". J. Biol. Chem. 276 (13): 9640–8. doi:10.1074/jbc.M008201200. PMID 11136725.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Morel AP, Sentis S, Bianchin C, Le Romancer M, Jonard L, Rostan MC, Rimokh R, Corbo L (2003). "BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms". J. Cell Sci. 116 (Pt 14): 2929–36. doi:10.1242/jcs.00480. PMID 12771185.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Dorfleutner A, Bryan NB, Talbott SJ, Funya KN, Rellick SL, Reed JC, Shi X, Rojanasakul Y, Flynn DC, Stehlik C (2007). "Cellular pyrin domain-only protein 2 is a candidate regulator of inflammasome activation". Infect. Immun. 75 (3): 1484–92. doi:10.1128/IAI.01315-06. PMC 1828547. PMID 17178784.

External links

• Human CNOT8 genome location and CNOT8 gene details page in the UCSC Genome Browser.