Brucella sRNA

Bacterial small RNAs (sRNA) are an important class of regulatory molecules in bacteria such as Brucella. They are often bound to the chaperone protein Hfq, which allows them to interact with mRNA(s). In Brucella suis 1330 RNA sequencing identified a novel list of 33 sRNAs and 62 Hfq-associated mRNAs.[1] In Brucella melitensis eight novel sRNA genes were identified using bioinformatic and experimental approach. One of them BSR0602 was found to modulate the intracellular survival of B. melitensis.[2] In another large-scale deep sequencing study 1321 sRNAs were identified in B. melitensis.[3] BSR0441 sRNA was further investigated in this study and shown to play role in the intracellular survival. sRNA BM-sr0117 from Brucella melitensis was identified and shown to be bound to and cleaved by Bm-RNase III.[4] AbcR and AbcR2 (orthologs of SmrC15 and SmrC16) were studied B. abortus.[5] Seven novel sRNAs were validated and their interaction with a putative target sequence was verified in B. abortus.[6]

Hfq protein regulates virB operon which is required for full virulence of the bacteria. It can bind to 5' untranslated region of virB transcriptional regulator BabR and mediate its effects on babR expression.[7]

gollark: Why would running at a power output it's designed for burn it out? It'll just waste energy slightly.
gollark: There is probably mental stuff which is both interesting and not conveniently consciously accessible, though.
gollark: If you mean that it can't produce information which isn't already available to your mind in some way, then that seems quite obviously true, yes.
gollark: I see.
gollark: I mysteriously have basically no autobiographical memory in general. Mostly I just ignore it.

See also

References
  1. Saadeh, B; et al. (2015). "Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing". J. Bacteriol. 198 (3): 427–435. doi:10.1128/JB.00711-15. PMC 4719455. PMID 26553849.
  2. Wang, Y; et al. (2015). "Identification of a Novel Small Non-Coding RNA Modulating the Intracellular Survival of Brucella melitensis". Front. Microbiol. 6: 164. doi:10.3389/fmicb.2015.00164. PMC 4365724. PMID 25852653.
  3. Zhong, Zhijun; Xu, Xiaoyang; Li, Xinran; Liu, Shiwei; Lei, Shuangshuang; Yang, Mingjuan; Yu, Jiuxuan; Yuan, Jiuyun; Ke, Yuehua (2016-01-01). "Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis". Scientific Reports. 6: 25123. doi:10.1038/srep25123. ISSN 2045-2322. PMC 4845025. PMID 27112796.
  4. Wu, CX; et al. (2016). "Characterization of ribonuclease III from Brucella". Gene. 579 (2): 183–192. doi:10.1016/j.gene.2015.12.068. PMID 26778206.
  5. Caswell, Clayton C.; Gaines, Jennifer M.; Ciborowski, Pawel; Smith, Derek; Borchers, Christoph H.; Roux, Christelle M.; Sayood, Khalid; Dunman, Paul M.; Roop Ii, R. Martin (2012-07-01). "Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308". Molecular Microbiology. 85 (2): 345–360. doi:10.1111/j.1365-2958.2012.08117.x. ISSN 1365-2958. PMC 3391331. PMID 22690807.
  6. Dong, Hao; Peng, Xiaowei; Wang, Ning; Wu, Qingmin (2014-05-01). "Identification of novel sRNAs in Brucella abortus 2308". FEMS Microbiology Letters. 354 (2): 119–125. doi:10.1111/1574-6968.12433. ISSN 1574-6968. PMID 24673258.
  7. Caswell, CC; Gaines, JM; Roop II, RM (2012). "The RNA chaperone Hfq independently coordinates expression of the VirB type IV secretion system and the LuxR-type regulator BabR in Brucella abortus 2308". J. Bacteriol. 194 (1): 3–14. doi:10.1128/JB.05623-11. PMC 3256608. PMID 22020650.
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