Artificial cerebrospinal fluid

Artificial cerebrospinal fluid (aCSF) is a buffer solution that is used experimentally to immerse isolated brains, brain slices, or exposed brain regions to supply oxygen, maintain osmolarity, and to buffer pH at biological levels. ACSF is commonly used for electrophysiology experiments to maintain the neurons that are being studied.

Composition

One protocol for electrophysiology recording suggests the following composition for aCSF, with the pH and oxygen level stabilized by bubbling with carbogen (95% O₂ and 5% CO₂):[1]

127 mM NaCl
1.0 mM KCl
1.2 mM KH₂PO₄
26 mM NaHCO₃
10 mM D-glucose
2.4 mM CaCl₂
1.3 mM MgCl₂

gollark: Windows.

gollark: I think that this whole "physical computing" thing teaches people that you can only program stuff on Arduinos and whatnot, which is completely wrong.

gollark: Yes.

gollark: `Shreya looks at the Arduino code on the machine and breaks up the structure inside her forever loop`

gollark: ... why block discord messages?

References

"Brain slice electrophysiology video protocol". abcam. Retrieved 28 March 2017.

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