Artificial cerebrospinal fluid

Artificial cerebrospinal fluid (aCSF) is a buffer solution that is used experimentally to immerse isolated brains, brain slices, or exposed brain regions to supply oxygen, maintain osmolarity, and to buffer pH at biological levels. ACSF is commonly used for electrophysiology experiments to maintain the neurons that are being studied.

Composition

One protocol for electrophysiology recording suggests the following composition for aCSF, with the pH and oxygen level stabilized by bubbling with carbogen (95% O2 and 5% CO2):[1]

  • 127 mM NaCl
  • 1.0 mM KCl
  • 1.2 mM KH2PO4
  • 26 mM NaHCO3
  • 10 mM D-glucose
  • 2.4 mM CaCl2
  • 1.3 mM MgCl2
gollark: So you want L+2r there to equal the total circumference of the circle?
gollark: It's a theta.
gollark: Er, sector, not area.
gollark: The *perimeter* of the area equals its total circumference?
gollark: <@379441093558927391> Could you be more specific, then?

References
  1. "Brain slice electrophysiology video protocol". abcam. Retrieved 28 March 2017.
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