Aniline Blue WS

Aniline Blue WS, also called aniline blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them.[1] It is a soluble dye used as a biological dye,[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.[4]

Water blue
Methyl blue

Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,[5] as well as to reveal callose structures in plant tissues.[6]

It can be used in the Mallory's connective tissue stain[5] and Gömöri trichrome stain. It is used in differential staining.

References
  1. "Stainsfile - Aniline blue WS". stainsfile.info. Archived from the original on 2019-02-09. Retrieved 2012-01-14.
  2. "Medical Definition of ANILINE BLUE". www.merriam-webster.com.
  3. "Archived copy". Archived from the original on 2008-05-26. Retrieved 2012-01-14.CS1 maint: archived copy as title (link)
  4. "aniline blue".
  5. "Stainsfile - Water blue". stainsfile.info. Archived from the original on 2012-02-07. Retrieved 2012-01-14.
  6. "Protocols - Staining with trypan blue and aniline blue - Felix Mauch's Group". Archived from the original on 22 June 2005.
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