Acid fuchsin

Acid fuchsin or fuchsine acid, (also called Acid Violet 19[1] and C.I. 42685[1]) is an acidic magenta dye with the chemical formula C₂₀H₁₇N₃Na₂O₉S₃. Acid fuchsin has wide use in histology,[1] and is one of the dyes used in Masson's trichrome stain.[2] This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with light green SF yellowish or methyl blue. It can also be used to identify growing bacteria.[3]

Acid fuchsin

Names
IUPAC name Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato-2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Identifiers
CAS Number	3244-88-0^Y
3D model (JSmol)	Interactive image
ChEBI	CHEBI:87052^Y
ChemSpider	16736101^Y
ECHA InfoCard	100.019.833
EC Number	221-816-5
PubChem CID	5464362
UNII	8RA6L21QTM^Y
CompTox Dashboard (EPA)	DTXSID8044418
InChI InChI=1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2/b19-11-,21-14?;;^Y Key: RZUBARUFLYGOGC-MTHOTQAESA-L^Y InChI=1/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-LOQRZINWBG
SMILES CC1=CC(=CC(=C1N)S(=O)(=O)[O-])/C(=C\2/C=CC(=[NH2+])C(=C2)S(=O)(=O)[O-])/C3=CC(=C(C=C3)N)S(=O)(=O)[O-].[Na+].[Na+]
Properties
Chemical formula	C20H17N3Na2O9S3
Molar mass	585.53 g·mol^−1
Hazards
GHS pictograms
GHS Signal word	Warning
GHS hazard statements	H315, H319, H335
GHS precautionary statements	P261, P264, P271, P280, P302+352, P304+340, P305+351+338, P312, P321, P332+313, P337+313, P362, P403+233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

See also

• Fuchsine
• New fuchsine
• Pararosanilin
• Verhoeff’s Stain

References

1. Lillie, Ralph Dougall (1977). H. J. Conn's Biological stains (9th ed.). Baltimore: Williams & Wilkins. p. 692.
2. Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4
3. Holman, W. L (1914). "The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria" (PDF). Journal of Infectious Diseases. 15: 227–233. doi:10.1093/infdis/15.1.227.