ARFGEF2

Brefeldin A-inhibited guanine nucleotide-exchange protein 2 is a protein that in humans is encoded by the ARFGEF2 gene.[5][6]

ARFGEF2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3L8N, 3SWV
Identifiers
Aliases	ARFGEF2, BIG2, PVNH2, dJ1164I10.1, ADP ribosylation factor guanine nucleotide exchange factor 2
External IDs	OMIM: 605371 MGI: 2139354 HomoloGene: 111880 GeneCards: ARFGEF2
Gene location (Human) Chr. Chromosome 20 (human)[1] Band 20q13.13 Start 48,921,711 bp[1] End 49,036,693 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 H3 Start 166,805,588 bp[2] End 166,898,052 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • guanyl-nucleotide exchange factor activity • GABA receptor binding • ARF guanyl-nucleotide exchange factor activity • myosin binding • GO:0001948 protein binding • protein kinase A regulatory subunit binding Cellular component • cytoplasm • recycling endosome • cytosol • endosome • Golgi apparatus • cell projection • membrane • Golgi membrane • dendritic spine • synapse • microtubule organizing center • cell junction • trans-Golgi network • dendrite • symmetric synapse • asymmetric synapse • perinuclear region of cytoplasm • cytoskeleton • cytoplasmic vesicle • axonemal microtubule Biological process • endomembrane system organization • GO:0007243 intracellular signal transduction • regulation of ARF protein signal transduction • endosome organization • receptor recycling • positive regulation of tumor necrosis factor production • protein transport • Golgi to plasma membrane transport • exocytosis • transport • regulation of molecular function • vesicle-mediated transport Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10564	99371
Ensembl	ENSG00000124198	ENSMUSG00000074582
UniProt	Q9Y6D5	A2A5R2
RefSeq (mRNA)	NM_006420	NM_001085495
RefSeq (protein)	NP_006411	NP_001078964
Location (UCSC)	Chr 20: 48.92 – 49.04 Mb	Chr 2: 166.81 – 166.9 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.[6]

Interactions

ARFGEF2 has been shown to interact with ARFGEF1,[7] PRKAR1A[8] and PRKAR2A.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000124198 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000074582 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Togawa A, Morinaga N, Ogasawara M, Moss J, Vaughan M (Apr 1999). "Purification and cloning of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors". The Journal of Biological Chemistry. 274 (18): 12308–15. doi:10.1074/jbc.274.18.12308. PMID 10212200.
6. "Entrez Gene: ARFGEF2 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)".
7. Yamaji R, Adamik R, Takeda K, Togawa A, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M (Mar 2000). "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (6): 2567–72. doi:10.1073/pnas.97.6.2567. PMC 15969. PMID 10716990.
8. Li H, Adamik R, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2003). "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1627–32. doi:10.1073/pnas.0337678100. PMC 149883. PMID 12571360.

External links

• Human ARFGEF2 genome location and ARFGEF2 gene details page in the UCSC Genome Browser.

Further reading

• Yamaji R, Adamik R, Takeda K, Togawa A, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M (Mar 2000). "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (6): 2567–72. doi:10.1073/pnas.97.6.2567. PMC 15969. PMID 10716990.
• Kamei Y, Takeda Y, Teramoto K, Tsutsumi O, Taketani Y, Watanabe K (Oct 2000). "Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members". Genomics. 69 (1): 113–9. doi:10.1006/geno.2000.6310. PMID 11013081.
• Pacheco-Rodriguez G, Moss J, Vaughan M (2002). "BIG1 and BIG2: brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors". Methods in Enzymology. 345: 397–404. doi:10.1016/S0076-6879(02)45032-X. PMID 11665623.
• Shinotsuka C, Yoshida Y, Kawamoto K, Takatsu H, Nakayama K (Mar 2002). "Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation". The Journal of Biological Chemistry. 277 (11): 9468–73. doi:10.1074/jbc.M112427200. PMID 11777925.
• Shinotsuka C, Waguri S, Wakasugi M, Uchiyama Y, Nakayama K (Jun 2002). "Dominant-negative mutant of BIG2, an ARF-guanine nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins". Biochemical and Biophysical Research Communications. 294 (2): 254–60. doi:10.1016/S0006-291X(02)00456-4. PMID 12051703.
• Li H, Adamik R, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2003). "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1627–32. doi:10.1073/pnas.0337678100. PMC 149883. PMID 12571360.
• Sheen VL, Ganesh VS, Topcu M, Sebire G, Bodell A, Hill RS, Grant PE, Shugart YY, Imitola J, Khoury SJ, Guerrini R, Walsh CA (Jan 2004). "Mutations in ARFGEF2 implicate vesicle trafficking in neural progenitor proliferation and migration in the human cerebral cortex". Nature Genetics. 36 (1): 69–76. doi:10.1038/ng1276. PMID 14647276.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (Jul 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Xu KF, Shen X, Li H, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2005). "Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70". Proceedings of the National Academy of Sciences of the United States of America. 102 (8): 2784–9. doi:10.1073/pnas.0409871102. PMC 549493. PMID 15705715.
• Lu J, Tiao G, Folkerth R, Hecht J, Walsh C, Sheen V (Jan 2006). "Overlapping expression of ARFGEF2 and Filamin A in the neuroependymal lining of the lateral ventricles: insights into the cause of periventricular heterotopia". The Journal of Comparative Neurology. 494 (3): 476–84. doi:10.1002/cne.20806. PMID 16320251.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Islam A, Shen X, Hiroi T, Moss J, Vaughan M, Levine SJ (Mar 2007). "The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles". The Journal of Biological Chemistry. 282 (13): 9591–9. doi:10.1074/jbc.M607122200. PMID 17276987.
• Kuroda F, Moss J, Vaughan M (Feb 2007). "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma". Proceedings of the National Academy of Sciences of the United States of America. 104 (9): 3201–6. doi:10.1073/pnas.0611696104. PMC 1805514. PMID 17360629.