If you plug a 10BASE-T device into a 10/100 switch, just that one device's port will run at 10mbps. All the ports connected to the 100BASE-TX devices will still go at 100mbps. Regardless of whether the switch is doing cut-through or store-and-forward switching, it operates each port at that port's native speed. It's not like the switch has to cycle all the ports to renegotiate the link speed of all ports down to 10/Half just because a 10/Half client was transmitting a broadcast or multicast. That would be nuts.
If a server on 100BASE-TX needs to send a lot of data to a client on 10BASE-T, the sending server could fill up the client's 10mbit link, but the server would still be able to use the other 90% of its own link to transfer data to other devices.
I could dream up a pathologically bad switch design where the switch only has one small pool of frame buffers shared by all ports, and uses a pathologically bad algorithm for choosing which frames to drop when the buffers fill up, where if any one 100mbps device was sending a lot of data to the 10mbps device, it could fill up all the switch's buffers and keep them dominated by that one traffic flow, causing all other traffic flows across the switch to suffer. But again, that's just trying to dream up a worst-case scenario. It doesn't seem likely that anyone would create a switch that bad.
You may have heard of a (misnamed) beast called a "10/100 dual-speed hub". Those devices are like a 10BASE-T hub bridged to a 100BASE-TX hub, with a scheme to share the same set of physical jacks. Think of it like this: When you plug in a device to a port, the port autonegotiates speed. If it negotiates 10mbps, it connects the port to an internal 10BASE-T hub circuit. If it negotiates 100mbps, it connects the port to an internal 100BASE-TX hub circuit. The two internal hub circuits are connected together via a bridge circuit (a bridge is a 2-port switch). But even if your 10/100 network box with all the RJ-45 jacks turns out to be one of these dual-speed hubs and not a true 10/100 switch, the 10BASE-T device still won't slow down the 100BASE-TX devices, because the bridge circuit in between will do the store-and-forward buffering in each direction.
Agreed. In a switched Ethernet network, only the 10BASE-T link will operate at 10Mbit/s. So, traffic between the 10Mbit/s device and any other (faster) device will only be transferred at 10MBit/s on the 10BASE-T link (or hop); all other hops should transfer at their appropriate bit-rate. – sblair – 2010-03-23T22:19:27.580