TRNT1

tRNA-nucleotidyltransferase 1, is an enzyme that in humans is encoded by the TRNT1 gene.[5][6][7] This enzyme adds the nucleotide sequence CCA to the 3' end of tRNA, using ATP and CTP as substrates. The sequence creates the binding site for an amino acid.[8]

TRNT1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1OU5, 4X4W
Identifiers
Aliases	TRNT1, CCA1, MtCCA, CGI-47, SIFD, tRNA nucleotidyl transferase 1, RPEM
External IDs	OMIM: 612907 MGI: 1917297 HomoloGene: 9333 GeneCards: TRNT1
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p26.2 Start 3,126,916 bp[1] End 3,150,879 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6|6 E1 Start 106,769,120 bp[2] End 106,782,474 bp[2]
Gene ontology Molecular function • CTP:3'-cytidine-tRNA cytidylyltransferase activity • transferase activity • nucleotide binding • tRNA binding • nucleotidyltransferase activity • ATP binding • CTP:tRNA cytidylyltransferase activity • RNA binding • 5'-3' RNA polymerase activity • ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Cellular component • mitochondrial matrix • intracellular • mitochondrion • nucleoplasm Biological process • RNA processing • tRNA 3'-terminal CCA addition • tRNA processing • tRNA 3'-end processing • mitochondrial tRNA 3'-end processing Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	51095	70047
Ensembl	ENSG00000072756	ENSMUSG00000013736
UniProt	Q96Q11	Q8K1J6
RefSeq (mRNA)	NM_001302946 NM_016000 NM_182916	NM_001242358 NM_001242360 NM_027296
RefSeq (protein)	NP_001289875 NP_886552 NP_001354250 NP_001354251 NP_001354252	NP_001229287 NP_001229289 NP_081572
Location (UCSC)	Chr 3: 3.13 – 3.15 Mb	Chr 6: 106.77 – 106.78 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000072756 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000013736 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (Aug 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.
6. Nagaike T, Suzuki T, Tomari Y, Takemoto-Hori C, Negayama F, Watanabe K, Ueda T (Oct 2001). "Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases". J Biol Chem. 276 (43): 40041–9. doi:10.1074/jbc.M106202200. PMID 11504732.
7. "Entrez Gene: TRNT1 tRNA nucleotidyl transferase, CCA-adding, 1".
8. Lizano E, Scheibe M, Rammelt C, Betat H, Mörl M (May 2008). "A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair". Biochimie. 90 (5): 762–72. doi:10.1016/j.biochi.2007.12.007. PMID 18226598.

Further reading

• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Reichert AS, Thurlow DL, Mörl M (2002). "A eubacterial origin for the human tRNA nucleotidyltransferase?". Biol. Chem. 382 (10): 1431–8. CiteSeerX 10.1.1.657.1235. doi:10.1515/BC.2001.176. PMID 11727826.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Augustin MA, Reichert AS, Betat H, et al. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328 (5): 985–94. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Lizano E, Schuster J, Müller M, et al. (2007). "A splice variant of the human CCA-adding enzyme with modified activity". J. Mol. Biol. 366 (4): 1258–65. doi:10.1016/j.jmb.2006.12.016. PMID 17204286.