SPDEF

SAM pointed domain-containing Ets transcription factor is a protein that in humans is encoded by the SPDEF gene.[5][6][7]

SPDEF
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DKX
Identifiers
Aliases	SPDEF, PDEF, bA375E1.3, SAM pointed domain containing ETS transcription factor
External IDs	OMIM: 608144 MGI: 1353422 HomoloGene: 8231 GeneCards: SPDEF
Gene location (Human) Chr. Chromosome 6 (human)[1] Band 6p21.31 Start 34,537,802 bp[1] End 34,556,333 bp[1]
Gene location (Mouse) Chr. Chromosome 17 (mouse)[2] Band 17|17 A3.3 Start 27,714,352 bp[2] End 27,728,955 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • DNA binding • GO:0001078, GO:0001214, GO:0001206 DNA-binding transcription repressor activity, RNA polymerase II-specific • sequence-specific DNA binding • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component • cell nucleus Biological process • multicellular organism development • cell differentiation • regulation of transcription, DNA-templated • negative regulation of transcription from RNA polymerase II promoter • negative regulation of cell fate commitment • lung goblet cell differentiation • intestinal epithelial cell development • transcription, DNA-templated • positive regulation of cell fate commitment • positive regulation of transcription from RNA polymerase II promoter • positive regulation of apoptotic process • regulation of transcription from RNA polymerase II promoter Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	25803	30051
Ensembl	ENSG00000124664	ENSMUSG00000024215
UniProt	O95238	Q9WTP3
RefSeq (mRNA)	NM_001252294 NM_012391	NM_013891 NM_001357728
RefSeq (protein)	NP_001239223 NP_036523	NP_038919 NP_001344657
Location (UCSC)	Chr 6: 34.54 – 34.56 Mb	Chr 17: 27.71 – 27.73 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM][7]

Interactions

SPDEF has been shown to interact with NKX3-1.[8]

The protein kinase CK2 has been shown to control the stability of PDEF.[9] Needed for the differentiation of both pulmonary and intestinal goblet cells.

References

1. GRCh38: Ensembl release 89: ENSG00000124664 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024215 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Oettgen P; Finger E; Sun Z; Akbarali Y; Thamrongsak U; Boltax J; Grall F; Dube A; Weiss A; Brown L; Quinn G; Kas K; Endress G; Kunsch C; Libermann TA (February 2000). "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression". J Biol Chem. 275 (2): 1216–25. doi:10.1074/jbc.275.2.1216. PMID 10625666.
6. Johannessen JV; Finseth I (July 1983). "Electron micrographs in five minutes". Ultrastruct Pathol. 4 (1): 97–100. doi:10.3109/01913128309140576. PMID 6857767.
7. "Entrez Gene: SPDEF SAM pointed domain containing ets transcription factor".
8. Chen, Hui; Nandi Asit K; Li Xiang; Bieberich Charles J (January 2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter". Cancer Res. United States. 62 (2): 338–40. ISSN 0008-5472. PMID 11809674.
9. Chisholm, Christine; Padmanabhan Achuth; Guan Bin; Li Xiang; Kalra Simran; Bieberich Charles J (2010). "Protein Kinase CK2 regulates the stability of prostate derived Ets factor". The Open Cancer Journal. Bentham Science. 3: 109–115. doi:10.2174/1874079001003010109.http://www.benthamscience.com/open/tocj/articles/V003/SI0022TOCJ/109TOCJ.pdf

Further reading

• Lance V (1976). "Studies on the annual reproductive cycle of the female cobra, Naja naja. Seasonal variation in plasma inorganic ions". Comparative Biochemistry and Physiology A. 53 (3): 285–9. doi:10.1016/S0300-9629(76)80037-0. PMID 2437.
• Yamada N; Tamai Y; Miyamoto H; Nozaki M (2000). "Cloning and expression of the mouse Pse gene encoding a novel Ets family member". Gene. 241 (2): 267–74. doi:10.1016/S0378-1119(99)00484-9. PMID 10675039.
• Chen H; Nandi AK; Li X; Bieberich CJ (2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter". Cancer Res. 62 (2): 338–40. PMID 11809674.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Thompson HG, Harris JW, Wold BJ, et al. (2003). "p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells". Oncogene. 22 (15): 2322–33. doi:10.1038/sj.onc.1206325. PMID 12700667.
• Feldman RJ, Sementchenko VI, Gayed M, et al. (2003). "Pdef expression in human breast cancer is correlated with invasive potential and altered gene expression". Cancer Res. 63 (15): 4626–31. PMID 12907642.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi:10.1038/nature02055. PMID 14574404.
• Ghadersohi A, Odunsi K, Lele S, et al. (2004). "Prostate derived Ets transcription factor shows better tumor-association than other cancer-associated molecules". Oncol. Rep. 11 (2): 453–8. doi:10.3892/or.11.2.453. PMID 14719083.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Chen H; Bieberich CJ (2005). "Structural and functional analysis of domains mediating interaction between NKX-3.1 and PDEF". J. Cell. Biochem. 94 (1): 168–77. doi:10.1002/jcb.20297. PMID 15523673.
• Wang Y, Feng L, Said M, et al. (2005). "Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site". Biochemistry. 44 (19): 7095–106. doi:10.1021/bi047352t. PMID 15882048.
• Fowler M, Borazanci E, McGhee L, et al. (2006). "RUNX1 (AML-1) and RUNX2 (AML-3) cooperate with prostate-derived Ets factor to activate transcription from the PSA upstream regulatory region". J. Cell. Biochem. 97 (1): 1–17. doi:10.1002/jcb.20664. PMID 16237704.
• Gunawardane RN, Sgroi DC, Wrobel CN, et al. (2006). "Novel role for PDEF in epithelial cell migration and invasion". Cancer Res. 65 (24): 11572–80. doi:10.1158/0008-5472.CAN-05-1196. PMC 2919290. PMID 16357167.
• Rodabaugh KJ, Mhawech-Fauceglia P, Groth J, et al. (2007). "Prostate-derived Ets factor is overexpressed in serous epithelial ovarian tumors". Int. J. Gynecol. Pathol. 26 (1): 10–5. doi:10.1097/01.pgp.0000225386.41244.bd. PMID 17197890.
• Park KS, Korfhagen TR, Bruno MD, et al. (2007). "SPDEF regulates goblet cell hyperplasia in the airway epithelium". J. Clin. Invest. 117 (4): 978–88. doi:10.1172/JCI29176. PMC 1810569. PMID 17347682.