RNF34

E3 ubiquitin-protein ligase RNF34 is an enzyme that in humans is encoded by the RNF34 gene.[5][6]

RNF34
Identifiers
Aliases	RNF34, CARP-1, CARP1, RFI, RIF, RIFF, hRFI, ring finger protein 34
External IDs	OMIM: 608299 MGI: 2153340 HomoloGene: 11839 GeneCards: RNF34
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q24.31 Start 121,400,041 bp[1] End 121,430,623 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 F Start 122,850,048 bp[2] End 122,871,291 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • metal ion binding • phosphatidylinositol phosphate binding • p53 binding • ubiquitin protein ligase binding • GO:0001948 protein binding • GO:1904264, GO:1904822, GO:0090622, GO:0090302 ubiquitin protein ligase activity • ubiquitin-protein transferase activity • transferase activity Cellular component • cytoplasm • endomembrane system • cell nucleus • membrane • cell membrane • nuclear speck • nucleoplasm • cytosol • nuclear body Biological process • negative regulation of signal transduction by p53 class mediator • negative regulation of extrinsic apoptotic signaling pathway via death domain receptors • nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway • protein K48-linked ubiquitination • negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis • apoptotic process • regulation of oxygen metabolic process • cellular response to cold • protein ubiquitination • proteasome-mediated ubiquitin-dependent protein catabolic process • ubiquitin-dependent protein catabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	80196	80751
Ensembl	ENSG00000170633	ENSMUSG00000029474
UniProt	Q969K3	Q99KR6
RefSeq (mRNA)	NM_194271 NM_001256858 NM_025126	NM_030564
RefSeq (protein)	NP_001243787 NP_079402 NP_919247	NP_085041
Location (UCSC)	Chr 12: 121.4 – 121.43 Mb	Chr 5: 122.85 – 122.87 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000170633 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000029474 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM (Jul 2002). "Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer". Oncogene. 21 (32): 5024–30. doi:10.1038/sj.onc.1205627. PMID 12118383.
6. "Entrez Gene: RNF34 ring finger protein 34".

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• McDonald ER, El-Deiry WS (2004). "Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth". Proc. Natl. Acad. Sci. U.S.A. 101 (16): 6170–5. doi:10.1073/pnas.0307459101. PMC 395941. PMID 15069192.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Sasaki S, Watanabe T, Konishi T, et al. (2005). "Effects of expression of hRFI on adenoma formation and tumor progression in colorectal adenoma-carcinoma sequence". J. Exp. Clin. Cancer Res. 23 (3): 507–12. PMID 15595643.
• Konishi T, Sasaki S, Watanabe T, et al. (2005). "Overexpression of hRFI (human ring finger homologous to inhibitor of apoptosis protein type) inhibits death receptor-mediated apoptosis in colorectal cancer cells". Mol. Cancer Ther. 4 (5): 743–50. doi:10.1158/1535-7163.MCT-05-0020. PMID 15897238.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.