Glycylpeptide N-tetradecanoyltransferase 2

Glycylpeptide N-tetradecanoyltransferase 2 known also as N-myristoyltransferase, is an enzyme (EC: 2.3.1.97) that in humans is encoded by the NMT2 gene.[5]

Human N-myristoyltransferase 2
Human N-myristoyltransferase isoform 2 (NMT2) (based on PDB: 4c2x)
Identifiers
Symbol	NMT2
Pfam	PF01233.14
InterPro	IPR022676
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

NMT2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4C2X
Identifiers
Aliases	NMT2, N-myristoyltransferase 2
External IDs	OMIM: 603801 MGI: 1202298 HomoloGene: 101539 GeneCards: NMT2
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10p13 Start 15,102,584 bp[1] End 15,168,693 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 A1 Start 3,284,212 bp[2] End 3,328,877 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • transferase activity, transferring acyl groups • glycylpeptide N-tetradecanoyltransferase activity Cellular component • cytoplasm • cell membrane • Golgi apparatus • membrane • extrinsic component of membrane • cytosol • host cell Biological process • regulation of rhodopsin mediated signaling pathway • GO:0046795 intracellular transport of virus • N-terminal protein myristoylation • N-terminal peptidyl-glycine N-myristoylation Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9397	18108
Ensembl	ENSG00000152465	ENSMUSG00000026643
UniProt	O60551	O70311
RefSeq (mRNA)	NM_001308295 NM_004808	NM_001290368 NM_001290369 NM_001290370 NM_008708
RefSeq (protein)	NP_001295224 NP_004799	NP_001277297 NP_001277298 NP_001277299 NP_032734
Location (UCSC)	Chr 10: 15.1 – 15.17 Mb	Chr 2: 3.28 – 3.33 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The 496-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases.[6]

Interactions

NMT2 has been shown to interact with:

• caspase 3[7]
• MARCKS[8]

See also

• N-myristoyltransferase 1

References

1. GRCh38: Ensembl release 89: ENSG00000152465 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026643 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Giang DK, Cravatt BF (Apr 1998). "A second mammalian N-myristoyltransferase". J Biol Chem. 273 (12): 6595–8. doi:10.1074/jbc.273.12.6595. PMID 9506952.
6. Thinon E, Serwa RA, Broncel M, Brannigan JA, Brassat U, Wright MH, Heal WP, Wilkinson AJ, Mann DJ, Tate EW (2014). "Global profiling of co- and post-translationally N-myristoylated proteomes in human cells". Nat Commun. 5: 4919. doi:10.1038/ncomms5919. PMC 4200515. PMID 25255805.
7. Selvakumar P, Sharma RK (2007). "Role of calpain and caspase system in the regulation of N-myristoyltransferase in human colon cancer (Review)". Int J Mol Med. 19 (5): 823–7. doi:10.3892/ijmm.19.5.823. PMID 17390089.
8. Selvakumar P, Lakshmikuttyamma A, Sharma RK (2009). "Biochemical characterization of bovine brain myristoyl-CoA:protein N-myristoyltransferase type 2". J Biomed Biotechnol. 2009: 907614. doi:10.1155/2009/907614. PMC 2737134. PMID 19746168.

Further reading

• Kolluri SK, Balduf C, Hofmann M, Göttlicher M (2002). "Novel target genes of the Ah (dioxin) receptor: transcriptional induction of N-myristoyltransferase 2". Cancer Res. 61 (23): 8534–9. PMID 11731439.
• Wright MH, Clough B, Rackham MD, Rangachari K, Brannigan JA, Grainger M, Moss DK, Bottrill AR, Heal WP, Broncel M, Serwa RA, Brady D, Mann DJ, Leatherbarrow RJ, Tewari R, Wilkinson AJ, Holder AA, Tate EW (2014). "Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach". Nat Chem. 6 (2): 112–21. doi:10.1038/nchem.1830. PMC 4739506. PMID 24451586.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Glycylpeptide N-tetradecanoyltransferase 2 (NMT2)