MGAT3

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the MGAT3 gene.[5][6]

MGAT3
Identifiers
Aliases	MGAT3, GNT-III, GNT3, mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
External IDs	OMIM: 604621 MGI: 104532 HomoloGene: 31088 GeneCards: MGAT3
Gene location (Human) Chr. Chromosome 22 (human)[1] Band 22q13.1 Start 39,457,012 bp[1] End 39,492,194 bp[1]
Gene location (Mouse) Chr. Chromosome 15 (mouse)[2] Band 15 E1|15 37.85 cM Start 80,173,721 bp[2] End 80,215,519 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • transferase activity, transferring glycosyl groups • beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Cellular component • integral component of membrane • Golgi membrane • Golgi apparatus • membrane Biological process • protein glycosylation • protein N-linked glycosylation • N-acetylglucosamine metabolic process • protein localization • regulation of cell migration • cellular response to oxidative stress • amyloid-beta metabolic process • cognition • positive regulation of protein localization to early endosome • negative regulation of lysosomal protein catabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4248	17309
Ensembl	ENSG00000128268	ENSMUSG00000042428
UniProt	Q09327	Q10470 Q5RKT9
RefSeq (mRNA)	NM_001098270 NM_002409	NM_010795
RefSeq (protein)	NP_001091740 NP_002400	NP_034925
Location (UCSC)	Chr 22: 39.46 – 39.49 Mb	Chr 15: 80.17 – 80.22 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000128268 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000042428 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ihara Y, Nishikawa A, Tohma T, Soejima H, Niikawa N, Taniguchi N (Oct 1993). "cDNA cloning, expression, and chromosomal localization of human N-acetylglucosaminyltransferase III (GnT-III)". J Biochem. 113 (6): 692–8. PMID 8370666.
6. "Entrez Gene: MGAT3 mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase".

Further reading

• Kim YJ, Park JH, Kim KS, et al. (1996). "Sequence analysis of the 5'-flanking region of the gene encoding human N-acetylglucosaminyltransferase III". Gene. 170 (2): 281–3. doi:10.1016/0378-1119(95)00818-7. PMID 8666260.
• Koyama N, Miyoshi E, Ihara Y, et al. (1996). "Human N-acetylglucosaminyltransferase III gene is transcribed from multiple promoters". Eur. J. Biochem. 238 (3): 853–61. doi:10.1111/j.1432-1033.1996.0853w.x. PMID 8706690.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.
• Ikeda Y, Koyota S, Ihara H, et al. (2000). "Kinetic basis for the donor nucleotide-sugar specificity of beta1, 4-N-acetylglucosaminyltransferase III". J. Biochem. 128 (4): 609–19. doi:10.1093/oxfordjournals.jbchem.a022793. PMID 11011143.
• Song EY, Kang SK, Lee YC, et al. (2002). "Expression of bisecting N-acetylglucosaminyltransferase-III in human hepatocarcinoma tissues, fetal liver tissues, and hepatoma cell lines of Hep3B and HepG2". Cancer Invest. 19 (8): 799–807. doi:10.1081/CNV-100107741. PMID 11768033.
• Shibukawa Y, Takahashi M, Laffont I, et al. (2003). "Down-regulation of hydrogen peroxide-induced PKC delta activation in N-acetylglucosaminyltransferase III-transfected HeLaS3 cells". J. Biol. Chem. 278 (5): 3197–203. doi:10.1074/jbc.M207870200. PMID 12427758.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Sasai K, Ikeda Y, Ihara H, et al. (2003). "Caveolin-1 regulates the functional localization of N-acetylglucosaminyltransferase III within the golgi apparatus". J. Biol. Chem. 278 (28): 25295–301. doi:10.1074/jbc.M301913200. PMID 12716887.
• Millar JK, Christie S, Porteous DJ (2004). "Yeast two-hybrid screens implicate DISC1 in brain development and function". Biochem. Biophys. Res. Commun. 311 (4): 1019–25. doi:10.1016/j.bbrc.2003.10.101. PMID 14623284.
• Isaji T, Gu J, Nishiuchi R, et al. (2004). "Introduction of bisecting GlcNAc into integrin alpha5beta1 reduces ligand binding and down-regulates cell adhesion and cell migration". J. Biol. Chem. 279 (19): 19747–54. doi:10.1074/jbc.M311627200. PMID 14998999.
• Collins JE, Wright CL, Edwards CA, et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biol. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMC 545604. PMID 15461802.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Fiala M, Liu PT, Espinosa-Jeffrey A, et al. (2007). "Innate immunity and transcription of MGAT-III and Toll-like receptors in Alzheimer's disease patients are improved by bisdemethoxycurcumin". Proc. Natl. Acad. Sci. U.S.A. 104 (31): 12849–54. doi:10.1073/pnas.0701267104. PMC 1937555. PMID 17652175.