MED27

Mediator of RNA polymerase II transcription subunit 27 is an enzyme that in humans is encoded by the MED27 gene.[5][6] It forms part of the Mediator complex.

MED27
Identifiers
Aliases	MED27, CRAP34, CRSP34, CRSP8, TRAP37, MED3, mediator complex subunit 27
External IDs	OMIM: 605044 MGI: 1916225 HomoloGene: 3152 GeneCards: MED27
Gene location (Human) Chr. Chromosome 9 (human)[1] Band 9q34.13 Start 131,852,928 bp[1] End 132,079,867 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2 B|2 19.84 cM Start 29,346,819 bp[2] End 29,524,793 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001105 transcription coactivator activity • GO:0001948 protein binding • GO:1904264, GO:1904822, GO:0090622, GO:0090302 ubiquitin protein ligase activity Cellular component • mediator complex • nucleolus • cell nucleus • transcription factor complex • nucleoplasm • ubiquitin ligase complex • cytosol Biological process • regulation of transcription from RNA polymerase II promoter • stem cell population maintenance • transcription initiation from RNA polymerase II promoter • regulation of transcription, DNA-templated • protein ubiquitination • transcription, DNA-templated • positive regulation of nucleic acid-templated transcription Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9442	68975
Ensembl	ENSG00000160563	ENSMUSG00000026799
UniProt	Q6P2C8	Q9DB40
RefSeq (mRNA)	NM_001253881 NM_001253882 NM_004269	NM_001290489 NM_026896 NM_029615 NM_001290490
RefSeq (protein)	NP_001240810 NP_001240811 NP_004260	NP_001277418 NP_001277419 NP_081172 NP_083891
Location (UCSC)	Chr 9: 131.85 – 132.08 Mb	Chr 2: 29.35 – 29.52 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Med27
Identifiers
Symbol	Med27
Pfam	PF11571
InterPro	IPR021627
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

The ubiquitous expression of Med27 mRNA suggests a universal requirement for Med27 in transcriptional initiation. Loss of Crsp34/Med27 decreases amacrine cell number, but increases the number of rod photoreceptor cells.[7]

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.[6]

See also

• Mediator complex

References

1. GRCh38: Ensembl release 89: ENSG00000160563 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026799 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature. 397 (6718): 446–50. doi:10.1038/17141. PMID 9989412.
6. "Entrez Gene: CRSP8 cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa".
7. Dürr K, Holzschuh J, Filippi A, Ettl AK, Ryu S, Shepherd IT, Driever W (October 2006). "Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development". Genetics. 174 (2): 693–705. doi:10.1534/genetics.105.055152. PMC 1602071. PMID 16582438.

This article incorporates text from the public domain Pfam and InterPro: IPR021627

Further reading

• Roth J, Krause M (1977). "[Relation between manifest bronchial obstruction and bronchial hyperreactivity (author's transl)]". Zeitschrift für Erkrankungen der Atmungsorgane. 146 (1): 12–6. PMID 1020353.
• Gress TM, Müller-Pillasch F, Geng M, et al. (1996). "A pancreatic cancer-specific expression profile". Oncogene. 13 (8): 1819–30. PMID 8895530.
• Malik S, Gu W, Wu W, et al. (2000). "The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs". Mol. Cell. 5 (4): 753–60. doi:10.1016/S1097-2765(00)80254-3. PMID 10882111.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Sato S, Tomomori-Sato C, Banks CA, et al. (2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". J. Biol. Chem. 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.
• Sato S, Tomomori-Sato C, Banks CA, et al. (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". J. Biol. Chem. 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
• Tomomori-Sato C, Sato S, Parmely TJ, et al. (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". J. Biol. Chem. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Humphray SJ, Oliver K, Hunt AR, et al. (2004). "DNA sequence and analysis of human chromosome 9". Nature. 429 (6990): 369–74. doi:10.1038/nature02465. PMC 2734081. PMID 15164053.
• Sato S, Tomomori-Sato C, Parmely TJ, et al. (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.