Francisco Mojica

Francisco Juan Martínez Mojica (Elche, October 5, 1963), usually known as Francisco JM Mojica, is a Spanish microbiologist at the University of Alicante in Spain, known for his research on the CRISPR gene editing technique.[1]

In 1993 repeats were observed in the archaeal organisms Haloferax and Haloarcula species, and their function was studied by Francisco Mojica.[2] Although his hypothesis turned out to be wrong, Mojica surmised at the time that the clustered repeats had a role in correctly segregating replicated DNA into daughter cells during cell division because plasmids and chromosomes with identical repeat arrays could not coexist in Haloferax volcanii. Transcription of the interrupted repeats was also noted for the first time. By 2000, Mojica's group had identified interrupted repeats in 20 species of microbes.

In 2001, Mojica and Ruud Jansen, who was searching for additional interrupted repeats, proposed the acronym CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) to alleviate the confusion stemming from the numerous acronyms used to describe the sequences in the scientific literature. In 2005 he published the first paper suggesting that CRISPR was an innate immune system from bacteria. This publication got rejected from 4 major Scientific journals.

Mojica was a winner of the 2017 Albany Medical Center Prize.[3]

Publications

(A selection from those mentioned in the institutional page of the Universidad de Alicante,[4] and those referred by Lander in the article The Heroes of CRISPR[5])

  • F.J.M. Mojica, R.A. Garrett. Discovery and Seminal Developments in the CRISPR field (2013). In: CRISPR-Cas Systems: RNA-mediated adaptive immunity in Bacteria and Archaea. Barrangou, R. and van der Oost, J. (Eds.) Springer-Verlag Berlin Heidelberg. Cap1. pp 1–31. ISBN 978-3-642-34656-9; DOI: 10.1007/978-3-642-34657-6_1.
  • Mojica, F.J.M., Juez, G., and Rodrı́guez-Valera, F. (1993). Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified PstI sites. Mol. Microbiol. 9, 613–621
  • Mojica, F.J.M., Ferrer, C., Juez, G., and Rodrı́guez-Valera, F. (1995). Long stretches of short tandem repeats are present in the largest replicons of the Archaea Haloferax mediterranei and Haloferax volcanii and could be involved in replicon partitioning. Mol. Microbiol. 17, 85–93.
  • Mojica, F.J.M., Dı́ez-Villaseñor, C., Soria, E., and Juez, G. (2000). Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria. Mol. Microbiol. 36, 244–246.
  • Mojica, F.J.M., Dı́ez-Villaseñor, C., Garcı́a-Martı́nez, J., and Soria, E. (2005). Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J. Mol. Evol. 60, 174–182.
  • Mojica, F.J.M., and Garrett, R.A. (2012). Discovery and Seminal Developments in the CRISPR Field. In CRISPR-Cas Systems, R. Barrangou and J. van der Oost, eds. (Berlin, Heidelberg: Springer Berlin Heidelberg), pp. 1–31.
  • Makarova K.S., Wolf Y.I., Alkhnbashi O., Costa F., Shah S., Saunders S.J., Barrangou R., Brouns S.J.J., Charpentier E., Haft D.H., Horvath P., Moineau S., Mojica F.J.M., Terns R.M., Terns M.A., White M.F., Yakunin A.F., Garrett R.A., van der Oost J., Backofen R., Koonin E.V. (2015). An updated evolutionary classification scheme for CRISPR-Cas systems. Nature Rev Microbiology, 13: 722-736.
  • García-Gutiérrez E., Almendros C., Mojica F.J.M., Guzmán N.M., García-Martínez J. (2015). CRISPR content correlates with the pathogenic potential of Escherichia coli. PLoS ONE, 10(7): e0131935.
  • Almendros C., Mojica F.J.M. (2015). Exploring CRISPR-mediated interference by transformation with plasmid mixtures: identification of target interference motifs in Escherichia coli. Methods in Molecular Biology, 1311: 161-70.
  • Almendros C., Mojica F.J.M., Díez-Villaseñor C., Guzmán N.M., García-Martínez J. (2014). CRISPR-Cas functional module exchange in Escherichia coli. mBIO, 5 (1): e00767-13.
  • Mojica, F.J.M.; Díez-Villaseñor, C. (2013). "Right of admission reserved, no matter the path". Trends in Microbiology. 21 (9). pp. 446–448. ISSN 0966-842X. Retrieved 17 August 2016.
  • Shah S.A., Erdmann S., Mojica F.J.M., Garrett R.A. (2013). Protospacer recognition motifs: mixed identities and functional diversity. RNA Biol, 10:891-899.
  • Díez-Villaseñor C., Guzmán N.M., Almendros C., García-Martínez J., Mojica F.J.M. (2013). CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli. RNA Biol, 10: 792-802.
  • Almendros C., Guzmán N.M., Díez-Villaseñor C., García-Martínez J. and Mojica F.J.M. (2012). Target Motifs Affecting Natural Immunity by a Constitutive CRISPR-Cas System in Escherichia coli. PLoS ONE, 7(11): e50797.
  • García-Heredia I., Martín-Cuadrado A.-B., Mojica F.J.M. , Santos F. , Mira A., Antón J. and Rodríguez-Valera F. (2012). Reconstructing Viral Genomes from the Environment Using Fosmid Clones: The Case of Haloviruses. PLoS ONE 7(3): e33802.
  • Kira S. Makarova, Daniel H. Haft, Rodolphe Barrangou, Stan Brouns, Emmanuelle Charpentier, Philippe Horvath, Sylvain Moineau, Francisco J. M. Mojica, Yuri I. Wolf, Alexander F. Yakunin, John van der Oost, and Eugene V. Koonin (2011). Evolution and classification of the CRISPR-Cas systems. Nature Rev Microbiology, 9: 467-477.
  • F.J.M. Mojica and C. Díez-Villaseñor (2010). The on-off switch of CRISPR immunity against phages in E. coli. Molecular Microbiology, 77:1341-1345.
  • S. Miquel, E. Peyretaillade, L. Claret, A. De Vallee, C. Dossat, B. Vacherie, E. Hajji Zineb, B. Segurens, V. Barbe, P. Sauvanet, C. Neut, J. Colombel, C. Medigue, F.J.M. Mojica, P. Peyret, R. Bonnet and A. Darfeuille-Michaud (2010). Complete genome sequence of Crohn's disease-associated adherent-invasive E. coli strain LF82. PLoS ONE, 5(9): e12714.
  • C. Díez-Villaseñor, C. Almendros, J. García-Martínez, and F.J.M. Mojica (2010). Diversity of CRISPR loci in Escherichia coli. Microbiology, 156: 1351-1361.
  • F. J. M. Mojica, C. Díez-Villaseñor, J. García-Martínez, C. Almendros (2009). Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology, 155:733-740.

References
  1. Ledford, Heidi (January 2017), "Five big mysteries about CRISPR's origins", Nature, 541 (7637): 280–282, doi:10.1038/541280a, PMID 28102279
  2. Mojica, F.J., Juez, G. & Rodríguez-Valera, F. (1993): Transcription at different salinities of Haloferax mediterranei sequences adjacent to partially modified PstI sites, Molecular Microbiology, Vol. 9, Nr. 3, page 613-621.
  3. Albany Medical Center. Gene Editing Pioneers Selected to Receive America’s Most Distinguished Prize in Medicine, August 15, 2017
  4. "Multidisciplinary Institute for Environmental - Francisco Juan Martínez Mojica", Universidad de Alicante, retrieved 1 August 2017
  5. Lander, Eric S. (2016), "The Heroes of CRISPR", Cell, 164 (1–2): 18–28, doi:10.1016/j.cell.2015.12.041, ISSN 0092-8674, PMID 26771483, retrieved 1 August 2017
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