EVI5

Ecotropic viral integration site 5 protein homolog is a protein that in humans is encoded by the EVI5 gene.[5][6]

EVI5
Identifiers
Aliases	EVI5, NB4S, EVI-5, ecotropic viral integration site 5
External IDs	OMIM: 602942 MGI: 104736 HomoloGene: 121902 GeneCards: EVI5
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1p22.1 Start 92,508,696 bp[1] End 92,792,404 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5 F|5 52.23 cM Start 107,744,795 bp[2] End 107,875,107 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • Rab GTPase binding • GO:0001948 protein binding • GO:0005097, GO:0005099, GO:0005100 GTPase activator activity Cellular component • cytoplasm • cytosol • spindle • cytoskeleton • cell nucleus • microtubule organizing center • endomembrane system • Golgi apparatus • intracellular membrane-bounded organelle Biological process • cell division • multicellular organism development • regulation of cilium assembly • GO:0043087, GO:0032313, GO:0032319, GO:0032314, GO:0043088 regulation of GTPase activity • cell cycle • cellular proliferation • retrograde transport, endosome to Golgi • regulation of vesicle fusion • intracellular protein transport • GO:0032861, GO:0032862, GO:0032856 activation of GTPase activity • GO:0032320, GO:0032321, GO:0032855, GO:0043089, GO:0032854 positive regulation of GTPase activity Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	7813	14020
Ensembl	ENSG00000067208	ENSMUSG00000011831
UniProt	O60447	P97366
RefSeq (mRNA)	NM_001308248 NM_005665 NM_001350197 NM_001350198 NM_001377210 NM_001377211 NM_001377212 NM_001377213	NM_007964 NM_001378824 NM_001378825
RefSeq (protein)	NP_001295177 NP_005656 NP_001337126 NP_001337127 NP_001364139 NP_001364140 NP_001364141 NP_001364142	n/a
Location (UCSC)	Chr 1: 92.51 – 92.79 Mb	Chr 5: 107.74 – 107.88 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Model organisms

Model organisms have been used in the study of EVI5 function. A conditional knockout mouse line called Evi5^{tm1a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.[7] Male and female animals underwent a standardized phenotypic screen[8] to determine the effects of deletion.[9][10][11][12] Additional screens performed: - In-depth immunological phenotyping[13]

Evi5 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[8][13]
Peripheral blood leukocytes 6 Weeks	Normal
Insulin	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal

References

1. GRCh38: Ensembl release 89: ENSG00000067208 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000011831 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Roberts T, Chernova O, Cowell JK (Jul 1998). "NB4S, a member of the TBC1 domain family of genes, is truncated as a result of a constitutional t(1;10)(p22;q21) chromosome translocation in a patient with stage 4S neuroblastoma". Human Molecular Genetics. 7 (7): 1169–78. doi:10.1093/hmg/7.7.1169. PMID 9618176.
6. "Entrez Gene: EVI5 ecotropic viral integration site 5".
7. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
8. "International Mouse Phenotyping Consortium".
9. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
13. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Dabbeekeh JT, Faitar SL, Dufresne CP, Cowell JK (Apr 2007). "The EVI5 TBC domain provides the GTPase-activating protein motif for RAB11". Oncogene. 26 (19): 2804–8. doi:10.1038/sj.onc.1210081. PMID 17099728.
• Westlake CJ, Junutula JR, Simon GC, Pilli M, Prekeris R, Scheller RH, Jackson PK, Eldridge AG (Jan 2007). "Identification of Rab11 as a small GTPase binding protein for the Evi5 oncogene". Proceedings of the National Academy of Sciences of the United States of America. 104 (4): 1236–41. doi:10.1073/pnas.0610500104. PMC 1773056. PMID 17229837.
• Faitar SL, Sossey-Alaoui K, Ranalli TA, Cowell JK (Jul 2006). "EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in the completion of cytokinesis". Experimental Cell Research. 312 (12): 2325–35. doi:10.1016/j.yexcr.2006.03.032. PMID 16764853.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (Apr 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Eldridge AG, Loktev AV, Hansen DV, Verschuren EW, Reimann JD, Jackson PK (Jan 2006). "The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1". Cell. 124 (2): 367–80. doi:10.1016/j.cell.2005.10.038. PMID 16439210.
• Faitar SL, Dabbeekeh JT, Ranalli TA, Cowell JK (Nov 2005). "EVI5 is a novel centrosomal protein that binds to alpha- and gamma-tubulin". Genomics. 86 (5): 594–605. doi:10.1016/j.ygeno.2005.06.002. PMID 16033705.
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, Li Y, Xu C, Fang R, Guegler K, Rao MS, Mandalam R, Lebkowski J, Stanton LW (Jun 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nature Biotechnology. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.