CHKA

Choline kinase alpha is an enzyme that in humans is encoded by the CHKA gene.[5][6][7]

CHKA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2CKO, 2CKP, 2CKQ, 2I7Q, 3F2R, 3G15, 3ZM9, 4BR3, 4CG8, 4CG9, 4CGA, 4DA5, 5AFV, 5EQE, 5EQP, 5EQY, 5FTG
Identifiers
Aliases	CHKA, CHK, CK, CKI, EK, choline kinase alpha
External IDs	OMIM: 118491 MGI: 107760 HomoloGene: 88575 GeneCards: CHKA
EC number	2.7.1.82
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11q13.2 Start 68,052,859 bp[1] End 68,121,444 bp[1]
Gene location (Mouse) Chr. Chromosome 19 (mouse)[2] Band 19 A|19 3.57 cM Start 3,851,773 bp[2] End 3,894,369 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • nucleotide binding • protein homodimerization activity • cholinesterase activity • signal transducer activity • choline binding • kinase activity • drug binding • ATP binding • ethanolamine kinase activity • choline kinase activity Cellular component • cytoplasm • cytosol Biological process • lipid transport • ethanolamine metabolic process • choline metabolic process • phosphorylation • lipid metabolism • response to 3-methylcholanthrene • phospholipid biosynthetic process • response to toxic substance • CDP-choline pathway • signal transduction • phosphatidylcholine biosynthetic process • phosphatidylethanolamine biosynthetic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1119	12660
Ensembl	ENSG00000110721	ENSMUSG00000024843
UniProt	P35790	O54804
RefSeq (mRNA)	NM_001277 NM_212469 NM_001376219 NM_001376220 NM_001376221 NM_001376222	NM_001025566 NM_001271496 NM_013490
RefSeq (protein)	NP_001268 NP_997634 NP_001363148 NP_001363149 NP_001363150 NP_001363151	NP_001258425 NP_038518
Location (UCSC)	Chr 11: 68.05 – 68.12 Mb	Chr 19: 3.85 – 3.89 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene.[7]

In melanocytic cells CHKA gene expression may be regulated by MITF.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000110721 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024843 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hosaka K, Tanaka S, Nikawa J, Yamashita S (Aug 1992). "Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation". FEBS Lett. 304 (2–3): 229–32. doi:10.1016/0014-5793(92)80625-Q. PMID 1618328.
6. Aoyama C, Liao H, Ishidate K (Mar 2004). "Structure and function of choline kinase isoforms in mammalian cells". Prog Lipid Res. 43 (3): 266–81. doi:10.1016/j.plipres.2003.12.001. PMID 15003397.
7. "Entrez Gene: CHKA choline kinase alpha".
8. Hoek KS, Schlegel NC, Eichhoff OM, et al. (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971.

External links

• Human CHKA genome location and CHKA gene details page in the UCSC Genome Browser.

Further reading

• Spanner S, Ansell GB (1979). "Choline kinase and ethanolamine kinase activity in the cytosol of nerve endings from rat forebrain". Biochem. J. 178 (3): 753–60. PMC 1186576. PMID 36885.
• Spanner S, Ansell GB (1978). "Choline and ethanolamine kinase activity in the cytoplasm of nerve endings from rat forebrain". Adv. Exp. Med. Biol. 101: 237–45. doi:10.1007/978-1-4615-9071-2_23. PMID 208357.
• Kaplan O, Cohen JS (1994). "Lymphocyte activation-31P magnetic resonance studies of energy metabolism and phospholipid pathways". ImmunoMethods. 4 (2): 139–47. doi:10.1006/immu.1994.1015. PMID 8069533.
• Price DJ, Rivnay B, Fu Y, et al. (1997). "Direct association of Csk homologous kinase (CHK) with the diphosphorylated site Tyr568/570 of the activated c-KIT in megakaryocytes". J. Biol. Chem. 272 (9): 5915–20. doi:10.1074/jbc.272.9.5915. PMID 9038210.
• Ross BM, Moszczynska A, Blusztajn JK, et al. (1997). "Phospholipid biosynthetic enzymes in human brain". Lipids. 32 (4): 351–8. doi:10.1007/s11745-997-0044-x. PMID 9113621.
• Nakagami K, Uchida T, Ohwada S, et al. (1999). "Increased choline kinase activity and elevated phosphocholine levels in human colon cancer". Jpn. J. Cancer Res. 90 (4): 419–24. doi:10.1111/j.1349-7006.1999.tb00764.x. PMC 5926083. PMID 10363580.
• Ramírez de Molina A, Penalva V, Lucas L, Lacal JC (2002). "Regulation of choline kinase activity by Ras proteins involves Ral-GDS and PI3K". Oncogene. 21 (6): 937–46. doi:10.1038/sj.onc.1205144. PMID 11840339.
• Dubois T, Howell S, Zemlickova E, Aitken A (2002). "Identification of casein kinase Ialpha interacting protein partners". FEBS Lett. 517 (1–3): 167–71. doi:10.1016/S0014-5793(02)02614-5. PMID 12062430.
• Ramírez de Molina A, Gutiérrez R, Ramos MA, et al. (2002). "Increased choline kinase activity in human breast carcinomas: clinical evidence for a potential novel antitumor strategy". Oncogene. 21 (27): 4317–22. doi:10.1038/sj.onc.1205556. PMID 12082619.
• Ramírez de Molina A, Rodríguez-González A, Gutiérrez R, et al. (2002). "Overexpression of choline kinase is a frequent feature in human tumor-derived cell lines and in lung, prostate, and colorectal human cancers". Biochem. Biophys. Res. Commun. 296 (3): 580–3. doi:10.1016/S0006-291X(02)00920-8. PMID 12176020.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Gallego-Ortega D, Ramirez De Molina A, Gutierrez R, et al. (2006). "Generation and characterization of monoclonal antibodies against choline kinase alpha and their potential use as diagnostic tools in cancer" (PDF). Int. J. Oncol. 29 (2): 335–40. doi:10.3892/ijo.29.2.335. PMID 16820874.
• Enaw JO, Zhu H, Yang W, et al. (2007). "CHKA and PCYT1A gene polymorphisms, choline intake and spina bifida risk in a California population". BMC Medicine. 4: 36. doi:10.1186/1741-7015-4-36. PMC 1770928. PMID 17184542.