ALS2CR8

Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein also known as calcium-response factor (CaRF) is a protein that in humans is encoded by the ALS2CR8 gene.[5][6]

CARF
Identifiers
Aliases	CARF, ALS2CR8, NYD-SP24, calcium responsive transcription factor
External IDs	OMIM: 607586 MGI: 2182269 HomoloGene: 11689 GeneCards: CARF
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q33.2 Start 202,912,214 bp[1] End 202,988,263 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 C2 Start 60,098,247 bp[2] End 60,153,953 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • GO:0000980 RNA polymerase II cis-regulatory region sequence-specific DNA binding • DNA binding • GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA polymerase II-specific Cellular component • nucleolus • nucleoplasm • cell nucleus Biological process • cellular response to calcium ion • positive regulation of transcription from RNA polymerase II promoter in response to calcium ion • regulation of transcription, DNA-templated • transcription, DNA-templated • cellular response to potassium ion • positive regulation of transcription from RNA polymerase II promoter • transcription from RNA polymerase II promoter Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	79800	241066
Ensembl	ENSG00000138380	ENSMUSG00000026017
UniProt	Q8N187	Q8VHI4
RefSeq (mRNA)	NM_001104586 NM_001282910 NM_001282911 NM_001282912 NM_024744 NM_001322427 NM_001322428 NM_001322429 NM_001322430 NM_001352676 NM_001352677 NM_001352678 NM_001352679	NM_001285463 NM_001285473 NM_139150
RefSeq (protein)	NP_001098056 NP_001269839 NP_001269840 NP_001269841 NP_001309356 NP_001309357 NP_001309358 NP_079020 NP_001339605 NP_001339606 NP_001339607 NP_001339608	NP_001272392 NP_001272402 NP_631889
Location (UCSC)	Chr 2: 202.91 – 202.99 Mb	Chr 1: 60.1 – 60.15 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Knocking out the Carf (Als2cr8) gene in mice results in learning associated deficits. Furthermore, Carf has been shown to function as a transcription factor that regulates the expression of BDNF.[7]

References

1. GRCh38: Ensembl release 89: ENSG00000138380 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026017 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hadano S, Hand CK, Osuga H, Yanagisawa Y, Otomo A, Devon RS, Miyamoto N, Showguchi-Miyata J, Okada Y, Singaraja R, Figlewicz DA, Kwiatkowski T, Hosler BA, Sagie T, Skaug J, Nasir J, Brown RH Jr, Scherer SW, Rouleau GA, Hayden MR, Ikeda JE (Oct 2001). "A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2". Nat Genet. 29 (2): 166–73. doi:10.1038/ng1001-166. PMID 11586298.
6. "Entrez Gene: ALS2CR8 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8".
7. Tao X, West AE, Chen WG, Corfas G, Greenberg ME (January 2002). "A calcium-responsive transcription factor, CaRF, that regulates neuronal activity-dependent expression of BDNF". Neuron. 33 (3): 383–95. doi:10.1016/S0896-6273(01)00561-X. PMID 11832226.

External links

• Human CARF genome location and CARF gene details page in the UCSC Genome Browser.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Tao X, West AE, Chen WG, et al. (2002). "A calcium-responsive transcription factor, CaRF, that regulates neuronal activity-dependent expression of BDNF". Neuron. 33 (3): 383–95. doi:10.1016/S0896-6273(01)00561-X. PMID 11832226.
• Hasan MK, Yaguchi T, Sugihara T, et al. (2002). "CARF is a novel protein that cooperates with mouse p19ARF (human p14ARF) in activating p53". J. Biol. Chem. 277 (40): 37765–70. doi:10.1074/jbc.M204177200. PMID 12154087.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.