VPS18

Vacuolar protein sorting-associated protein 18 homolog is a protein that in humans is encoded by the VPS18 gene.[5][6][7]

VPS18
Identifiers
Aliases	VPS18, PEP3, CORVET/HOPS core subunit, VPS18 core subunit of CORVET and HOPS complexes
External IDs	OMIM: 608551 MGI: 2443626 HomoloGene: 13302 GeneCards: VPS18
Gene location (Human) Chr. Chromosome 15 (human)[1] Band 15q15.1 Start 40,894,450 bp[1] End 40,903,975 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 E5 Start 119,288,740 bp[2] End 119,298,453 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • protein binding, bridging • metal ion binding • GO:0001948 protein binding • actin binding • syntaxin binding Cellular component • HOPS complex • AP-3 adaptor complex • endosome • late endosome • membrane • late endosome membrane • autophagosome • lysosomal membrane • early endosome • actin filament • CORVET complex • lysosome • cytoplasmic vesicle • clathrin-coated vesicle • presynapse • glutamatergic synapse Biological process • endosome to lysosome transport • endosome organization • autophagy • protein transport • viral entry • intracellular protein transport • lysosome organization • vesicle-mediated transport • vesicle docking involved in exocytosis • regulation of SNARE complex assembly • transport • regulation of synaptic vesicle exocytosis • vacuole organization Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	57617	228545
Ensembl	ENSG00000104142	ENSMUSG00000034216
UniProt	Q9P253	Q8R307
RefSeq (mRNA)	NM_020857	NM_172269
RefSeq (protein)	NP_065908 NP_065908.1	NP_758473
Location (UCSC)	Chr 15: 40.89 – 40.9 Mb	Chr 2: 119.29 – 119.3 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.[7]

Interactions

VPS18 has been shown to interact with VPS11[8] and STX7.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000104142 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000034216 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Huizing M, Didier A, Walenta J, Anikster Y, Gahl WA, Krämer H (March 2001). "Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33". Gene. 264 (2): 241–7. doi:10.1016/S0378-1119(01)00333-X. PMID 11250079.
6. Yogosawa S, Hatakeyama S, Nakayama KI, Miyoshi H, Kohsaka S, Akazawa C (Dec 2005). "Ubiquitylation and degradation of serum-inducible kinase by hVPS18, a RING-H2 type ubiquitin ligase". J Biol Chem. 280 (50): 41619–27. doi:10.1074/jbc.M508397200. PMID 16203730.
7. "Entrez Gene: VPS18 vacuolar protein sorting 18 homolog (S. cerevisiae)".
8. Kim BY, Krämer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C (August 2001). "Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7". J. Biol. Chem. 276 (31): 29393–402. doi:10.1074/jbc.M101778200. PMID 11382755.

Further reading

• Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.
• Kim BY, Krämer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C (2001). "Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7". J. Biol. Chem. 276 (31): 29393–402. doi:10.1074/jbc.M101778200. PMID 11382755.
• Kim BY, Ueda M, Kominami E, Akagawa K, Kohsaka S, Akazawa C (2004). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochem. Biophys. Res. Commun. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Yogosawa S, Kawasaki M, Wakatsuki S, Kominami E, Shiba Y, Nakayama K, Kohsaka S, Akazawa C (2006). "Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability". Biochem. Biophys. Res. Commun. 350 (1): 82–90. doi:10.1016/j.bbrc.2006.09.013. PMID 16996030.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.