Texas Red

Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.[1][2] Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. The powder is dark purple. Solutions can be excited by a dye laser tuned to 595-605 nm, or less efficiently a krypton laser at 567 nm. The absorption extinction coefficient at 596 nm is about 85,000 M−1cm−1.

This article is about the dye. For other uses, see Texas Red (disambiguation).
Texas Red
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.245.455
Properties
C31H29ClN2O6S2
Molar mass 625.15 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references
The solution in the bottle (15 ml) has 0.009 mg/ml Texas Red in DMF. Bulk powder is dark purple.

The compound is usually a mixture of two monosulfonyl chlorides, i.e., as pictured, or with the SO3 and SO2Cl groups exchanged. It can be used as a marker of proteins, with which it easily forms conjugates via the sulfonyl chloride (SO2Cl) group. In water, the sulfonyl chloride group of unreacted Texas Red molecules hydrolyses to sulfonate and the molecule becomes the very water-soluble sulforhodamine 101 which is easy to wash out selectively. This is one of the advantages of conjugating with Texas Red vs. using a rhodamine-isothiocyanate for conjugation.

A protein with the Texas Red chromophore attached can then itself act as a fluorescent labeling agent; an antibody with a fluorescent marker attached will bind to a specific antigen and then show the location of the antigens as shining spots when irradiated. It is relatively bright, and therefore can be used to detect even weakly expressed antigens. Other molecules can be labeled by Texas Red as well, e.g., various toxins. The dye dissolves very well in water as well as other polar solvents, e.g., Dimethylformamide, acetonitrile.

Texas Red, attached to a strand of DNA or RNA, can be used as a molecular beacon for highlighting specific sequences of DNA. Texas Red can be linked with another fluorophore. A tandem conjugate of Texas Red with R-phycoerythrin (PE-Texas Red) is often used.

Fluorophores, like Texas Red, are commonly used in molecular biology techniques like quantitative RT-PCR and cellular assays.[3]

Newer rhodamine derivatives, such as Alexa 594 and DyLight 594, have been tailored to match the excitation and emission spectra of Texas Red and are used in various chemical and biological applications where greater photostability or higher fluorescence intensity are needed.

References
  1. Titus JA; Haugland R; Sharrow SO; Segal DM (1982). "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies". J. Immunol. Methods. 50 (2): 193–204. doi:10.1016/0022-1759(82)90225-3. PMID 6806389.
  2. Sulforhodamine 101 acid chloride Sigma-Aldrich product information
  3. Ahmad AI; Ghasemi JB (2007). "New FRET primers for quantitative real-time PCR". Anal Bioanal Chem. 387 (8): 2737–43. doi:10.1007/s00216-007-1123-4. PMID 17308892.
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