TRAF3IP3

TRAF3-interacting JNK-activating modulator is a protein that in humans is encoded by the TRAF3IP3 gene.[5][6]

TRAF3IP3
Identifiers
Aliases	TRAF3IP3, T3JAM, TRAF3 interacting protein 3
External IDs	OMIM: 608255 MGI: 2441706 HomoloGene: 11885 GeneCards: TRAF3IP3
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1q32.2 Start 209,756,032 bp[1] End 209,782,320 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 H6 Start 193,175,453 bp[2] End 193,201,703 bp[2]
RNA expression pattern More reference expression data
Orthologs
Species	Human	Mouse
Entrez	80342	215243
Ensembl	ENSG00000009790	ENSMUSG00000037318
UniProt	Q9Y228	Q8C0G2
RefSeq (mRNA)	NM_001287754 NM_025228 NM_001320143 NM_001320144	NM_153137
RefSeq (protein)	NP_001274683 NP_001307072 NP_001307073 NP_079504	NP_694777
Location (UCSC)	Chr 1: 209.76 – 209.78 Mb	Chr 1: 193.18 – 193.2 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Model organisms

Traf3ip3 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Abnormal[7]
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Eye Histopathology	Normal
Salmonella infection	Normal[8]
Citrobacter infection	Abnormal[9]
All tests and analysis from[10][11]

Model organisms have been used in the study of TRAF3IP3 function. A conditional knockout mouse line, called Traf3ip3^{tm1a(KOMP)Wtsi}[12][13] was generated as part of the International Knockout Mouse Consortium program—a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[14][15][16]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[10][17] Twenty three tests were carried out on homozygous mutant mice and two significant abnormalities were observed.[10] Males had a decrease in white blood cell count and females had an increased susceptibility to bacterial infection.[10]

Interactions

TRAF3IP3 has been shown to interact with STRN,[18] MOBKL3,[18] STK24[18] and FAM40A.[18]

References

1. GRCh38: Ensembl release 89: ENSG00000009790 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000037318 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Dadgostar H, Doyle SE, Shahangian A, Garcia DE, Cheng G (Oct 2003). "T3JAM, a novel protein that specifically interacts with TRAF3 and promotes the activation of JNK(1)". FEBS Letters. 553 (3): 403–7. doi:10.1016/S0014-5793(03)01072-X. PMID 14572659.
6. "Entrez Gene: TRAF3IP3 TRAF3 interacting protein 3".
7. "Haematology data for Traf3ip3". Wellcome Trust Sanger Institute.
8. "Salmonella infection data for Traf3ip3". Wellcome Trust Sanger Institute.
9. "Citrobacter infection data for Traf3ip3". Wellcome Trust Sanger Institute.
10. Gerdin, AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
11. Mouse Resources Portal, Wellcome Trust Sanger Institute.
12. "International Knockout Mouse Consortium".
13. "Mouse Genome Informatics".
14. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
15. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
16. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
17. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
18. Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez A, Chaudhry S, Chen GI, Sicheri F, Nesvizhskii AI, Aebersold R, Raught B, Gingras AC (Jan 2009). "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein". Molecular & Cellular Proteomics. 8 (1): 157–71. doi:10.1074/mcp.M800266-MCP200. PMC 2621004. PMID 18782753.

Further reading

• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Vasilescu J, Zweitzig DR, Denis NJ, Smith JC, Ethier M, Haines DS, Figeys D (Jan 2007). "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells". Journal of Proteome Research. 6 (1): 298–305. CiteSeerX 10.1.1.401.4220. doi:10.1021/pr060438j. PMID 17203973.
• Zhang C, Xie L, Cheng H, Wang Y (Feb 2007). "TRAF3 interacts with Smac/DIABLO and enhances the proapoptotic effect of Smac/DIABLO in cytoplasm". Acta Biochimica et Biophysica Sinica. 39 (2): 108–16. doi:10.1111/j.1745-7270.2007.00259.x. PMID 17277885.
• Ma X, Wang X, Gao X, Wang L, Lu Y, Gao P, Deng W, Yu P, Ma J, Guo J, Cheng H, Zhang C, Shi T, Ma D (Sep 2007). "Identification of five human novel genes associated with cell proliferation by cell-based screening from an expressed cDNA ORF library". Life Sciences. 81 (14): 1141–51. doi:10.1016/j.lfs.2007.08.006. PMID 17868742.