Sirius Red

Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid.[1] It has the molecular formula C₄₅H₂₆N₁₀Na₆O₂₁S₆.

Sirius Red

Names
IUPAC name Hexasodium (3E)-4-oxo-7-[[(6E)-5-oxo-7-sulfonato-6-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]hydrazinylidene]naphthalen-2-yl]carbamoylamino]-3-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]hydrazinylidene]naphthalene-2-sulfonate
Other names Direct Red 80; Picrosirius Red
Identifiers
CAS Number	2610-10-8
3D model (JSmol)	Interactive image
ECHA InfoCard	100.018.208
PubChem CID	9571131
CompTox Dashboard (EPA)	DTXSID8044628
SMILES C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N/N=C/3\C(=O)C4=C(C=C3S(=O)(=O)[O-])C=C(C=C4)NC(=O)NC5=CC6=C(C(=O)/C(=N\NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])/C(=C6)S(=O)(=O)[O-])C=C5)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+]
Properties
Chemical formula	C45H26N10Na6O21S6
Molar mass	1373.05 g·mol^−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.[2]

In bright field microscopy the following can be observed:

• The nuclei in yellow
• The cytoplasm in yellow
• Collagen fibers in red
• Muscular fibers in yellow
• Red blood cells in yellow

Sirius red staining in rat liver, where the red color indicates collagen deposition[3]

See also

• Collagen Hybridizing Peptide, a peptide that stains denatured collagen in tissues

References

1. Dapson, Richard W.; Fagan, C.; Kiernan, John A.; Wickersham, T.W. (March 2011). "Certification procedures for sirius red F3B". Biotechnic & Histochemistry. 86 (3): 133–9. doi:10.3109/10520295.2011.570277. PMID 21417582. Retrieved 2015-08-21.
2. "Sirius red". Histalim. Retrieved 2015-09-22.
3. Dwivedi, Durgesh Kumar; Jena, G B (November 2018). "Glibenclamide protects against thioacetamide-induced hepatic damage in Wistar rat: investigation on NLRP3, MMP-2, and stellate cell activation". Naunyn-Schmiedeberg's Archives of Pharmacology. 391 (11): 1257–1274. doi:10.1007/s00210-018-1540-2. PMID 30066023.