Simulated fluorescence process algorithm

The Simulated Fluorescence Process (SFP) is a computing algorithm used for scientific visualization of 3D data from, for example, fluorescence microscopes. By modeling a physical light/matter interaction process an image is computed showing the data as it would have appeared in reality when viewed under these conditions.

Principle

The algorithm considers a virtual light source producing excitation light that illuminates the object. This casts shadows either on parts of the object itself or on other objects below it. The interaction between the excitation light and the object provokes the emission light, that also interacts with the object before it finally reaches the eye of the viewer.

gollark: Observe, our bee compactor.
gollark: 100-axis political/gender compass WHEN?
gollark: We have fully automated bee eugenics.
gollark: None are safe.
gollark: The UK is at least less <:bees:724389994663247974> than poland.

References

H. T. M. van der Voort, G. J. Brakenhoff and M. W. Baarslag. "Three-dimensional visualization methods for confocal microscopy", Journal of Microscopy, Vol. 153, Pt 2, February 1989, pp. 123–132.

Noordmans, Herke Jan, Hans TM van der Voort, and Arnold WM Smeulders. "Spectral volume rendering." IEEE transactions on visualization and computer graphics 6.3 (2000): 196-207.


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