SENP8

Sentrin-specific protease 8 is an enzyme that in humans is encoded by the SENP8 gene.[5][6][7]

SENP8
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1XT9, 2BKQ, 2BKR
Identifiers
Aliases	SENP8, DEN1, NEDP1, PRSC2, SUMO/sentrin peptidase family member, NEDD8 specific, SUMO peptidase family member, NEDD8 specific
External IDs	OMIM: 608659 MGI: 1918849 HomoloGene: 14084 GeneCards: SENP8
Gene location (Human) Chr. Chromosome 15 (human)[1] Band 15q23 Start 72,114,258 bp[1] End 72,143,692 bp[1]
Gene location (Mouse) Chr. Chromosome 9 (mouse)[2] Band 9|9 B Start 59,734,259 bp[2] End 59,750,710 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • peptidase activity • cysteine-type peptidase activity • hydrolase activity • NEDD8-specific protease activity • endopeptidase activity Cellular component • cytosol • cell nucleus Biological process • proteolysis • protein deubiquitination • protein desumoylation • post-translational modification Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	123228	71599
Ensembl	ENSG00000166192	ENSMUSG00000051705
UniProt	Q96LD8	Q9D2Z4
RefSeq (mRNA)	NM_001166340 NM_001172109 NM_001172110 NM_001172111 NM_145204	NM_001172068 NM_001172069 NM_001172070 NM_001172071 NM_027838
RefSeq (protein)	NP_001159812 NP_001165580 NP_001165581 NP_001165582 NP_660205	NP_001165539 NP_001165540 NP_001165541 NP_001165542 NP_082114
Location (UCSC)	Chr 15: 72.11 – 72.14 Mb	Chr 9: 59.73 – 59.75 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003).[supplied by OMIM][7]

References

1. GRCh38: Ensembl release 89: ENSG00000166192 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000051705 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Mendoza HM, Shen LN, Botting C, Lewis A, Chen J, Ink B, Hay RT (Jul 2003). "NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins". J Biol Chem. 278 (28): 25637–43. doi:10.1074/jbc.M212948200. PMID 12730221.
6. Gan-Erdene T, Nagamalleswari K, Yin L, Wu K, Pan ZQ, Wilkinson KD (Jul 2003). "Identification and characterization of DEN1, a deneddylase of the ULP family". J Biol Chem. 278 (31): 28892–900. doi:10.1074/jbc.M302890200. PMID 12759362.
7. "Entrez Gene: SENP8 SUMO/sentrin specific peptidase family member 8".

Further reading

• Mikolajczyk J, Drag M, Békés M, Cao JT, Ronai Z, Salvesen GS (2007). "Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs". J. Biol. Chem. 282 (36): 26217–24. doi:10.1074/jbc.M702444200. PMID 17591783.
• Drag M, Mikolajczyk J, Krishnakumar IM, Huang Z, Salvesen GS (2008). "Activity profiling of human deSUMOylating enzymes (SENPs) with synthetic substrates suggests an unexpected specificity of two newly characterized members of the family". Biochem. J. 409 (2): 461–9. doi:10.1042/BJ20070940. PMID 17916063.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Wu K, Yamoah K, Dolios G, et al. (2003). "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1". J. Biol. Chem. 278 (31): 28882–91. doi:10.1074/jbc.M302888200. PMID 12759363.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Xirodimas DP, Saville MK, Bourdon JC, et al. (2004). "Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity". Cell. 118 (1): 83–97. doi:10.1016/j.cell.2004.06.016. PMID 15242646.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Reverter D, Wu K, Erdene TG, et al. (2005). "Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1". J. Mol. Biol. 345 (1): 141–51. doi:10.1016/j.jmb.2004.10.022. PMID 15567417.
• Shen LN, Liu H, Dong C, et al. (2005). "Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1". EMBO J. 24 (7): 1341–51. doi:10.1038/sj.emboj.7600628. PMC 1142549. PMID 15775960.

External links

• Overview of all the structural information available in the PDB for UniProt: Q96LD8 (Sentrin-specific protease 8) at the PDBe-KB.