Runt domain

Available protein structures:
Pfam	structures / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	structure summary
PDB	1eaqA:48-182 1hjbC:60-182 1hjcD:60-182 1io4C:60-182 1eaoA:48-182 1eanA:48-182 1e50E:50-182 1co1A:61-175 1h9dC:50-182 1ljmB:51-181 1cmoA:52-178

Runt domain
Identifiers
Symbol	Runt
Pfam	PF00853
InterPro	IPR013524
SCOPe	1cmo / SUPFAM
Available protein structures:
Pfam	structures / ECOD
PDB	RCSB PDB; PDBe; PDBj
PDBsum	structure summary
PDB	1eaqA:48-182 1hjbC:60-182 1hjcD:60-182 1io4C:60-182 1eaoA:48-182 1eanA:48-182 1e50E:50-182 1co1A:61-175 1h9dC:50-182 1ljmB:51-181 1cmoA:52-178

The Runt domain is an evolutionary conserved protein domain.[1]

The AML1/RUNX1 gene is rearranged by the t(8;21) translocation in acute myeloid leukemia.[2] The gene is highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit gene.[2] The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction.

In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has a C-terminal region rich in proline and serine residues. The protein (known as acute myeloid leukemia 1 protein, oncogene AML-1, core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and promoters.

The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role in the development of normal hematopoiesis. CBF is a nuclear protein expressed in numerous tissue types, except brain and heart; highest levels have been found to occur in thymus, bone marrow and peripheral blood.

This domain occurs towards the N-terminus of the proteins in this entry.

Examples

Human genes encoding proteins containing this domain include:

RUNX1, RUNX2, RUNX3

gollark: I fear easy home production of deadly viruses or something.

gollark: Nobody* needs that.

gollark: Just remove the fingerprint bit.

gollark: Maybe you can make it smaller if you strip out all the random historical retroviruses and retrotransposons. Unless it's analogous to really poorly written code and needs all of it or it'll break mysteriously on line 173012.

gollark: I mean, it's still probably small enough to fit on a cheap USB stick.

References

Kagoshima H, Shigesada K, Satake M, Ito Y, Miyoshi H, Ohki M, Pepling M, Gergen P (October 1993). "The Runt domain identifies a new family of heteromeric transcriptional regulators". Trends Genet. 9 (10): 338–41. doi:10.1016/0168-9525(93)90026-E. PMID 8273148.
Hirai H, Shimizu K, Miyoshi H, Ohira M, Mitani K, Imai T, Yokoyama K, Soeda E, Ohki M (1995). "Alternative splicing and genomic structure of the AML1 gene involved in acute myeloid leukemia". Nucleic Acids Res. 23 (14): 2762–2769. doi:10.1093/nar/23.14.2762. PMC 307102. PMID 7651838.

This article incorporates text from the public domain Pfam and InterPro: IPR013524

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[pmid8273148-1] Kagoshima H, Shigesada K, Satake M, Ito Y, Miyoshi H, Ohki M, Pepling M, Gergen P (October 1993). "The Runt domain identifies a new family of heteromeric transcriptional regulators". Trends Genet. 9 (10): 338–41. doi:10.1016/0168-9525(93)90026-E. PMID 8273148.

[PUB00004459-2] Hirai H, Shimizu K, Miyoshi H, Ohira M, Mitani K, Imai T, Yokoyama K, Soeda E, Ohki M (1995). "Alternative splicing and genomic structure of the AML1 gene involved in acute myeloid leukemia". Nucleic Acids Res. 23 (14): 2762–2769. doi:10.1093/nar/23.14.2762. PMC 307102. PMID 7651838.