RNF10

RING finger protein 10 is a protein that in humans is encoded by the RNF10 gene.[5]

RNF10
Identifiers
Aliases	RNF10, RIE2, ring finger protein 10
External IDs	OMIM: 615998 MGI: 1859162 HomoloGene: 40990 GeneCards: RNF10
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q24.31 Start 120,533,480 bp[1] End 120,577,588 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5 F|5 56.01 cM Start 115,241,412 bp[2] End 115,272,898 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • GO:0001948 protein binding • metal ion binding • ubiquitin-protein transferase activity Cellular component • cytoplasm • cell nucleus Biological process • positive regulation of transcription, DNA-templated • positive regulation of myelination • regulation of transcription, DNA-templated • transcription, DNA-templated • negative regulation of Schwann cell proliferation • positive regulation of transcription from RNA polymerase II promoter • protein autoubiquitination Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9921	50849
Ensembl	ENSG00000022840	ENSMUSG00000041740
UniProt	Q8N5U6	Q3UIW5
RefSeq (mRNA)	NM_014868 NM_001330474	NM_016698 NM_001302448 NM_001302449
RefSeq (protein)	NP_001317403 NP_055683	NP_001289377 NP_001289378 NP_057907
Location (UCSC)	Chr 12: 120.53 – 120.58 Mb	Chr 5: 115.24 – 115.27 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known.[5]

Model organisms

Rnf10 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Abnormal[6]
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Abnormal[7]
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Abnormal[8]
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Abnormal[9]
Peripheral blood lymphocytes	Normal
Micronucleus test	Abnormal
Heart weight	Normal
Salmonella infection	Normal[10]
Citrobacter infection	Normal[11]
All tests and analysis from[12][13]

Model organisms have been used in the study of RNF10 function. A conditional knockout mouse line, called Rnf10^{tm1a(KOMP)Wtsi}[14][15] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[16][17][18]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[12][19] Twenty two tests were carried out on mutant mice and five significant abnormalities were observed.[12] Homozygous mutant animals displayed increased chromosomal stability in a micronucleus test. Females also had increased body weight, an increased amount of total body fat and an abnormal complete blood count. Males additionally displayed an increase in eating behavior.[12]

References

1. GRCh38: Ensembl release 89: ENSG00000022840 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000041740 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: RNF10 ring finger protein 10".
6. "Body weight data for Rnf10". Wellcome Trust Sanger Institute.
7. "Indirect calorimetry data for Rnf10". Wellcome Trust Sanger Institute.
8. "DEXA data for Rnf10". Wellcome Trust Sanger Institute.
9. "Haematology data for Rnf10". Wellcome Trust Sanger Institute.
10. "Salmonella infection data for Rnf10". Wellcome Trust Sanger Institute.
11. "Citrobacter infection data for Rnf10". Wellcome Trust Sanger Institute.
12. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
13. Mouse Resources Portal, Wellcome Trust Sanger Institute.
14. "International Knockout Mouse Consortium".
15. "Mouse Genome Informatics".
16. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
17. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
18. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
19. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (Jun 2002). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Research. 9 (3): 99–106. CiteSeerX 10.1.1.500.923. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N (Oct 1996). "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Research. 3 (5): 321–9, 341–54. doi:10.1093/dnares/3.5.321. PMID 9039502.
• Seki N, Hattori A, Sugano S, Muramatsu M, Saito T (2000). "cDNA cloning, expression profile, and genomic structure of human and mouse RNF10/Rnf 10 genes, encoding a novel RING finger protein". Journal of Human Genetics. 45 (1): 38–42. doi:10.1007/s100380050007. PMID 10697961.
• Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070.
• Lin J, Friesen MT, Bocangel P, Cheung D, Rawszer K, Wigle JT (Jul 2005). "Characterization of Mesenchyme Homeobox 2 (MEOX2) transcription factor binding to RING finger protein 10". Molecular and Cellular Biochemistry. 275 (1–2): 75–84. doi:10.1007/s11010-005-0823-3. PMID 16335786.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.