RASGRP1

RAS guanyl-releasing protein 1 is a protein that in humans is encoded by the RASGRP1 gene.[5][6][7]

RASGRP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4L9M, 4L9U
Identifiers
Aliases	RASGRP1, CALDAG-GEFI, CALDAG-GEFII, RASGRP, hRasGRP1, RAS guanyl releasing protein 1, IMD64
External IDs	OMIM: 603962 MGI: 1314635 HomoloGene: 4195 GeneCards: RASGRP1
Gene location (Human) Chr. Chromosome 15 (human)[1] Band 15q14 Start 38,488,103 bp[1] End 38,565,575 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2 E5|2 59.19 cM Start 117,279,993 bp[2] End 117,343,001 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • calcium ion binding • protein homodimerization activity • phosphatidylcholine binding • zinc ion binding • phosphatidylserine binding • metal ion binding • diacylglycerol binding • lipid binding • Ras guanyl-nucleotide exchange factor activity • guanyl-nucleotide exchange factor activity • GO:0005097, GO:0005099, GO:0005100 GTPase activator activity Cellular component • cytoplasm • Golgi apparatus • endoplasmic reticulum membrane • membrane • mast cell granule • Golgi membrane • endoplasmic reticulum • cytosol • cell membrane • intracellular Biological process • cell differentiation • positive regulation of protein phosphorylation • positive regulation of T cell differentiation in thymus • GO:0007243 intracellular signal transduction • regulation of phospholipase C activity • positive regulation of MAP kinase activity • positive regulation of natural killer cell differentiation • inflammatory response to antigenic stimulus • vesicle transport along microtubule • GO:0032861, GO:0032862, GO:0032856 activation of GTPase activity • GO:0043087, GO:0032313, GO:0032319, GO:0032314, GO:0043088 regulation of GTPase activity • cytokine production • positive regulation of Ras protein signal transduction • positive regulation of natural killer cell activation • positive regulation of JNK cascade • secretory granule localization • MAPK cascade • regulation of phosphatidylinositol 3-kinase signaling • GO:0032320, GO:0032321, GO:0032855, GO:0043089, GO:0032854 positive regulation of GTPase activity • positive regulation of interferon-gamma secretion • positive regulation of granulocyte macrophage colony-stimulating factor production • protein oligomerization • positive regulation of ERK1 and ERK2 cascade • regulation of ERK1 and ERK2 cascade • positive regulation of tumor necrosis factor production • Ras protein signal transduction • mast cell degranulation • regulation of p38MAPK cascade • positive regulation of natural killer cell mediated cytotoxicity • signal transduction • small GTPase mediated signal transduction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10125	19419
Ensembl	ENSG00000172575	ENSMUSG00000027347
UniProt	O95267	Q9Z1S3
RefSeq (mRNA)	NM_001128602 NM_001306086 NM_005739	NM_011246
RefSeq (protein)	NP_001122074 NP_001293015 NP_005730	NP_035376
Location (UCSC)	Chr 15: 38.49 – 38.57 Mb	Chr 2: 117.28 – 117.34 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

RAS guanyl nucleotide-releasing protein (RASGRP) is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation.[7]

Gene

Alternatively spliced transcript variants encoding different isoforms have been identified. The corresponding rat gene rbc7, which lacks a 5-prime exon, represents a 5-prime and 3-prime truncated version of a larger normal rat transcript that encodes a predicted 90-kD protein. This shorter transcript has not been found in humans.[7]

Clinical significance

In November 2016 a 12-year-old patient was hospitalized for repetitive infections. Scientists have assumed that a genetic problem might be the reason. More specifically, the genetic cause is a defect of the RASGRP1 gene which makes it inactive. .

RASGRP1 plays a role in the functions of natural killer cell dyneins. Since dyneins are motor proteins, their function is to circulate the elements inside the cells. Dr. Orange's laboratory studies have established a functional link between the defects of natural killer cells and dyneins, which in combination with Other observations led doctors to try the drug lenalidommide to treat the patient. The drug was able to reverse certain effects of the mutation RASGRP1.

References

1. GRCh38: Ensembl release 89: ENSG00000172575 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027347 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Bottorff D, Ebinu J, Stone JC (May 1999). "RasGRP, a Ras activator: mouse and human cDNA sequences and chromosomal positions". Mamm Genome. 10 (4): 358–61. doi:10.1007/s003359901001. PMID 10087292.
6. Kawasaki H, Springett GM, Toki S, Canales JJ, Harlan P, Blumenstiel JP, Chen EJ, Bany IA, Mochizuki N, Ashbacher A, Matsuda M, Housman DE, Graybiel AM (Nov 1998). "A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia". Proc Natl Acad Sci U S A. 95 (22): 13278–83. doi:10.1073/pnas.95.22.13278. PMC 23782. PMID 9789079.
7. "Entrez Gene: RASGRP1 RAS guanyl releasing protein 1 (calcium and DAG-regulated)".

Further reading

• Dower NA, Stang SL, Bottorff DA, Ebinu JO, Dickie P, Ostergaard HJ, Stone JC (2000). "RasGRP is essential for mouse thymocyte differentiation and TCR signaling". Nature Immunology. 1 (4): 317–21. doi:10.1038/79766. PMID 11017103.
• Priatel JJ, Teh SJ, Dower NA, Stone JC, et al. (2002). "RasGRP1 Transduces Low-Grade TCR Signals Which Are Critical For T Cell Development, Homeostasis and Differentiation". Immunity. 17 (5): 617–27. doi:10.1016/S1074-7613(02)00451-X. PMID 12433368.
• Priatel JJ, Chen X, Zenewicz LA, Shen H, Harder KW, Horwitz MS, et al. (2007). "Chronic Immunodeficiency in Mice Lacking RasGRP1 Results in CD4 T Cell Immune Activation and Exhaustion". J. Immunol. 179 (4): 2143–52. doi:10.4049/jimmunol.179.4.2143. PMID 17675473.
• Priatel JJ, Chen X, Dhanji S, Abraham N, et al. (2006). "RasGRP1 Transmits Pro-Differentiation TCR Signaling that is Crucial for CD4 T Cell Development". J. Immunol. 177 (3): 1470–80. doi:10.4049/jimmunol.177.3.1470. PMID 16849453.
• Priatel JJ, Chen X, Huang YH, Chow MT, Zenewicz LA, Coughlin JJ, Shen H, Stone JC, Tan R, et al. (2010). "RasGRP1 regulates antigen-induced developmental programming by naive CD8 T cells". J. Immunol. 184 (2): 666–76. doi:10.4049/jimmunol.0803521. PMID 20007535.
• Ebinu JO, Bottorff DA, Chan EY, et al. (1998). "RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs". Science. 280 (5366): 1082–6. doi:10.1126/science.280.5366.1082. PMID 9582122.
• Topham MK, Prescott SM (2001). "Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism". J. Cell Biol. 152 (6): 1135–43. doi:10.1083/jcb.152.6.1135. PMC 2199204. PMID 11257115.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Bivona TG, Pérez De Castro I, Ahearn IM, et al. (2003). "Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1". Nature. 424 (6949): 694–8. doi:10.1038/nature01806. PMID 12845332.
• Madani S, Hichami A, Cherkaoui-Malki M, et al. (2004). "Diacylglycerols containing Omega 3 and Omega 6 fatty acids bind to RasGRP and modulate MAP kinase activation". J. Biol. Chem. 279 (2): 1176–83. doi:10.1074/jbc.M306252200. PMID 14583629.
• Klinger MB, Guilbault B, Goulding RE, Kay RJ (2005). "Deregulated expression of RasGRP1 initiates thymic lymphomagenesis independently of T-cell receptors". Oncogene. 24 (16): 2695–704. doi:10.1038/sj.onc.1208334. PMID 15829980.
• Liu Y, Zhu M, Nishida K, et al. (2007). "An essential role for RasGRP1 in mast cell function and IgE-mediated allergic response". J. Exp. Med. 204 (1): 93–103. doi:10.1084/jem.20061598. PMC 2118421. PMID 17190838.
• Roose JP, Mollenauer M, Ho M, et al. (2007). "Unusual interplay of two types of Ras activators, RasGRP and SOS, establishes sensitive and robust Ras activation in lymphocytes". Mol. Cell. Biol. 27 (7): 2732–45. doi:10.1128/MCB.01882-06. PMC 1899892. PMID 17283063.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
• Pasvolsky R, Feigelson SW, Kilic SS, et al. (2007). "A LAD-III syndrome is associated with defective expression of the Rap-1 activator CalDAG-GEFI in lymphocytes, neutrophils, and platelets". J. Exp. Med. 204 (7): 1571–82. doi:10.1084/jem.20070058. PMC 2118641. PMID 17576779.
• Kosco KA, Cerignoli F, Williams S, et al. (2007). "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1". Mol. Immunol. 45 (2): 510–22. doi:10.1016/j.molimm.2007.05.024. PMID 17658605.
• Yasuda S, Stevens RL, Terada T, et al. (2007). "Defective expression of Ras guanyl nucleotide-releasing protein 1 in a subset of patients with systemic lupus erythematosus". J. Immunol. 179 (7): 4890–900. doi:10.4049/jimmunol.179.7.4890. PMID 17878389.

External links

• RASGRP1 human gene location in the UCSC Genome Browser.
• RASGRP1 human gene details in the UCSC Genome Browser.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.