RAP1B

Ras-related protein Rap-1b, also known as GTP-binding protein smg p21B, is a protein that in humans is encoded by the RAP1B gene.[5][6][7][8]

RAP1B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3BRW, 3CF6, 4DXA, 4HDO, 4HDQ, 4M8N, 4MGI, 4MGK, 4MGY, 4MGZ, 4MH0
Identifiers
Aliases	RAP1B, K-REV, RAL1B, member of RAS oncogene family
External IDs	OMIM: 179530 MGI: 894315 HomoloGene: 68719 GeneCards: RAP1B
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q15 Start 68,610,855 bp[1] End 68,671,901 bp[1]
Gene location (Mouse) Chr. Chromosome 10 (mouse)[2] Band 10|10 D2 Start 117,813,871 bp[2] End 117,846,035 bp[2]
Gene ontology Molecular function • nucleotide binding • GDP binding • GO:0032403 macromolecular complex binding • GTP binding • GO:0001948 protein binding • GTPase activity Cellular component • cytoplasm • cytosol • membrane • intercellular junction • cell membrane • lipid droplet • intracellular • cell junction • membrane raft • extracellular exosome • azurophil granule membrane Biological process • establishment of endothelial barrier • microvillus assembly • regulation of cell junction assembly • Rap protein signal transduction • regulation of establishment of cell polarity • cellular response to cAMP • signal transduction • neutrophil degranulation • interleukin-12-mediated signaling pathway • small GTPase mediated signal transduction • cellular proliferation • response to carbohydrate • cellular response to drug • negative regulation of calcium ion-dependent exocytosis • positive regulation of ERK1 and ERK2 cascade • cellular response to organic cyclic compound • cellular response to gonadotropin-releasing hormone • negative regulation of synaptic vesicle exocytosis Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5908	215449
Ensembl	ENSG00000127314	ENSMUSG00000052681
UniProt	P61224	Q99JI6
RefSeq (mRNA)	NM_015646 NM_001010942 NM_001251917 NM_001251918 NM_001251921 NM_001251922	NM_024457
RefSeq (protein)	NP_001010942 NP_001238846 NP_001238847 NP_001238850 NP_001238851 NP_056461 NP_001010942.1 NP_056461.1	NP_077777
Location (UCSC)	Chr 12: 68.61 – 68.67 Mb	Chr 10: 117.81 – 117.85 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000127314 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000052681 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: RAP1B".
6. Pizon V, Lerosey I, Chardin P, Tavitian A (August 1988). "Nucleotide sequence of a human cDNA encoding a ras-related protein (rap1B)". Nucleic Acids Res. 16 (15): 7719. doi:10.1093/nar/16.15.7719. PMC 338441. PMID 3137530.
7. Matsui Y, Kikuchi A, Kawata M, Kondo J, Teranishi Y, Takai Y (January 1990). "Molecular cloning of smg p21B and identification of smg p21 purified from bovine brain and human platelets as smg p21B". Biochem. Biophys. Res. Commun. 166 (2): 1010–6. doi:10.1016/0006-291X(90)90911-6. PMID 2105724.
8. Rousseau-Merck MF, Pizon V, Tavitian A, Berger R (1990). "Chromosome mapping of the human RAS-related RAP1A, RAP1B, and RAP2 genes to chromosomes 1p12----p13, 12q14, and 13q34, respectively". Cytogenet. Cell Genet. 53 (1): 2–4. doi:10.1159/000132883. PMID 2108841.

Further reading

• Rehmann H, Arias-Palomo E, Hadders MA, et al. (2008). "Structure of Epac2 in complex with a cyclic AMP analogue and RAP1B". Nature. 455 (7209): 124–7. Bibcode:2008Natur.455..124R. doi:10.1038/nature07187. PMID 18660803.
• Gyan E, Frew M, Bowen D, et al. (2005). "Mutation in RAP1 is a rare event in myelodysplastic syndromes". Leukemia. 19 (9): 1678–80. doi:10.1038/sj.leu.2403882. PMID 16118622.
• Schwamborn JC, Müller M, Becker AH, Püschel AW (2007). "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity". EMBO J. 26 (5): 1410–22. doi:10.1038/sj.emboj.7601580. PMC 1817628. PMID 17318188.
• Carmona G, Göttig S, Orlandi A, et al. (2009). "Role of the small GTPase Rap1 for integrin activity regulation in endothelial cells and angiogenesis". Blood. 113 (2): 488–97. doi:10.1182/blood-2008-02-138438. PMID 18805968.
• Shattil SJ (2005). "Integrins and Src: dynamic duo of adhesion signaling". Trends Cell Biol. 15 (8): 399–403. doi:10.1016/j.tcb.2005.06.005. PMID 16005629.
• Malchinkhuu E, Sato K, Maehama T, et al. (2009). "Role of Rap1B and tumor suppressor PTEN in the negative regulation of lysophosphatidic acid--induced migration by isoproterenol in glioma cells". Mol. Biol. Cell. 20 (24): 5156–65. doi:10.1091/mbc.E09-08-0692. PMC 2793292. PMID 19864456.
• Daumke O, Weyand M, Chakrabarti PP, et al. (2004). "The GTPase-activating protein Rap1GAP uses a catalytic asparagine". Nature. 429 (6988): 197–201. Bibcode:2004Natur.429..197D. doi:10.1038/nature02505. PMID 15141215.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Edreira MM, Li S, Hochbaum D, et al. (2009). "Phosphorylation-induced conformational changes in Rap1b: allosteric effects on switch domains and effector loop". J. Biol. Chem. 284 (40): 27480–6. doi:10.1074/jbc.M109.011312. PMC 2785677. PMID 19651783.
• Scrima A, Thomas C, Deaconescu D, Wittinghofer A (2008). "The Rap-RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues". EMBO J. 27 (7): 1145–53. doi:10.1038/emboj.2008.30. PMC 2265111. PMID 18309292.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Canobbio I, Trionfini P, Guidetti GF, et al. (2008). "Targeting of the small GTPase Rap2b, but not Rap1b, to lipid rafts is promoted by palmitoylation at Cys176 and Cys177 and is required for efficient protein activation in human platelets". Cell. Signal. 20 (9): 1662–70. doi:10.1016/j.cellsig.2008.05.016. PMID 18582561.
• Bernardi B, Guidetti GF, Campus F, et al. (2006). "The small GTPase Rap1b regulates the cross talk between platelet integrin alpha2beta1 and integrin alphaIIbbeta3". Blood. 107 (7): 2728–35. doi:10.1182/blood-2005-07-3023. PMC 1895386. PMID 16357324.
• Matsuse M, Mitsutake N, Rogounovitch T, et al. (2009). "Mutation analysis of RAP1 gene in papillary thyroid carcinomas". Endocr. J. 56 (1): 161–4. doi:10.1507/endocrj.k08e-244. PMID 18948674.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Li YH, Werner H, Püschel AW (2008). "Rheb and mTOR regulate neuronal polarity through Rap1B". J. Biol. Chem. 283 (48): 33784–92. doi:10.1074/jbc.M802431200. PMC 2662285. PMID 18842593.
• Lova P, Campus F, Lombardi R, et al. (2004). "Contribution of protease-activated receptors 1 and 4 and glycoprotein Ib-IX-V in the G(i)-independent activation of platelet Rap1B by thrombin". J. Biol. Chem. 279 (24): 25299–306. doi:10.1074/jbc.M313199200. PMID 15078882.
• Yan J, Li F, Ingram DA, Quilliam LA (2008). "Rap1a is a key regulator of fibroblast growth factor 2-induced angiogenesis and together with Rap1b controls human endothelial cell functions". Mol. Cell. Biol. 28 (18): 5803–10. doi:10.1128/MCB.00393-08. PMC 2546931. PMID 18625726.
• Campus F, Lova P, Bertoni A, et al. (2005). "Thrombopoietin complements G(i)- but not G(q)-dependent pathways for integrin {alpha}(IIb){beta}(3) activation and platelet aggregation". J. Biol. Chem. 280 (26): 24386–95. doi:10.1074/jbc.M501174200. PMID 15863506.
• Defilippi P, Di Stefano P, Cabodi S (2006). "p130Cas: a versatile scaffold in signaling networks". Trends Cell Biol. 16 (5): 257–63. doi:10.1016/j.tcb.2006.03.003. PMID 16581250.