PrrB/RsmZ RNA family

The PrrB/RsmZ RNA family are a group of related non-coding RNA molecules found in bacteria. PrrB RNA is able to phenotypically complement gacS and gacA mutants and is itself regulated by the GacS-GacA two-component signal transduction system.[1] Inactivation of the prrB gene in Pseudomonas fluorescens F113 resulted in a significant reduction of 2, 4-diacetylphloroglucinol (Phl) and hydrogen cyanide (HCN) production, while increased metabolite production was observed when prrB was overexpressed.[1] The prrB gene sequence contains a number of imperfect repeats of the consensus sequence 5′-AGGA-3′, and sequence analysis predicted a complex secondary structure featuring multiple putative stem-loops with the consensus sequences predominantly positioned at the single-stranded regions at the ends of the stem-loops. This structure is similar to the CsrB and RsmB regulatory RNAs (CsrB/RsmB RNA family),[1] suggesting this RNA also interacts with a CsrA-like protein.

PrrB/RsmZ RNA family
Predicted secondary structure and sequence conservation of PrrB_RsmZ
Identifiers
SymbolPrrB_RsmZ
RfamRF00166
Other data
RNA typeGene; sRNA
Domain(s)Bacteria
GO0006401 0006109
SO0000655
PDB structuresPDBe

Studies in Legionella pneumophila have shown that the ncRNAs RsmY and RsmZ together with the proteins LetA and CsrA are involved in a regulatory cascade. Also, it appears that these ncRNAs are regulated by RpoS sigma-factor.[2]

See also

References
  1. Aarons S, Abbas A, Adams C, Fenton A, O'Gara F (2000). "A regulatory RNA (PrrB RNA) modulates expression of secondary metabolite genes in Pseudomonas fluorescens F113". J. Bacteriol. 182 (14): 3913–3919. doi:10.1128/JB.182.14.3913-3919.2000. PMC 94573. PMID 10869066.
  2. Rasis M, Segal G (May 2009). "The LetA-RsmYZ-CsrA regulatory cascade, together with RpoS and PmrA, post-transcriptionally regulates stationary phase activation of Legionella pneumophila Icm/Dot effectors". Mol. Microbiol. 72 (4): 995–1010. doi:10.1111/j.1365-2958.2009.06705.x. PMID 19400807. Archived from the original on 2013-01-05.

Further reading
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