Photoactive yellow protein

In molecular biology, the PYP domain (photoactive yellow protein) is a p-coumaric acid-binding protein domain. They are present in various proteins in bacteria.

PYP
Identifiers
SymbolPYP
PfamPF00989
InterProIPR012130
SMARTSM00091
PROSITEPS50112
SCOPe55786 / SUPFAM
CDDcd00130

PYP is a highly soluble globular protein with an alpha/beta fold structure. It is a member of the PAS domain superfamily, which also contains a variety of other kinds of photosensory proteins.

PYP was first discovered in 1985.[1]

A recently (2016) developed chemogenetic system named FAST (Fluorescence-Activating and absorption Shifting Tag) was engineered from PYP to specifically and reversibly bind a series of hydroxybenzylidene rhodanine (HBR) derivatives for their fluorogenic properties. Upon interaction with FAST, the fluorogen is locked into a fluorescent conformation unlike when in solution. This new protein labelling system is used in a variety of microscopy and cytometry setups.[2]

References
  1. Meyer TE (January 1985). "Isolation and characterization of soluble cytochromes, ferredoxins and other chromophoric proteins from the halophilic phototrophic bacterium Ectothiorhodospira halophila". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 806 (1): 175–83. doi:10.1016/0005-2728(85)90094-5. PMID 2981543.
  2. Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht CG, et al. (January 2016). "Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo". Proceedings of the National Academy of Sciences of the United States of America. 113 (3): 497–502. doi:10.1073/pnas.1513094113. PMC 4725535. PMID 26711992.

Further reading
  • Imamoto Y, Kataoka M (2007). "Structure and photoreaction of photoactive yellow protein, a structural prototype of the PAS domain superfamily". Photochemistry and Photobiology. 83 (1): 40–9. doi:10.1562/2006-02-28-IR-827. PMID 16939366.
  • Overview of all the structural information available in the PDB for UniProt: P16113 (Photoactive yellow protein) at the PDBe-KB.
This article incorporates text from the public domain Pfam and InterPro: IPR012130
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