Parasutterella

Parasutterella is a genus of Gram-negative, circular/rod-shaped, obligate anaerobic, non-spore forming bacteria from the Proteobacteria phylum, Betaproteobacteria class and the family Sutterellaceae.[1][2] Previously, this genus was considered "unculturable,"[1] meaning that it could not be characterized through conventional laboratory techniques, such as grow in culture due its unique requirements of anaerobic environment. The genus was initially discovered through 16S rRNA sequencing and bioinformatics analysis (a methodology to analyze the microbiome). By analyzing the sequence similarity, Parasutterella was determined to be related most closely to the genus Sutterella and previously classified in the family Alcaligenaceae.

Parasutterella
Scientific classification
Kingdom:
Bacteria
Phylum:
Proteobacteria
Class:
Betaproteobacteria
Order:
Burkholderiales
Family:
Sutterellaceae
Genus:
Parasutterella

Nagai et al. 2009
Species

Parasutterella excrementihominis
Parasutterella secunda

In 2009, Parasutterella excrementihominis was cultured and characterized.[1] In 2011, another species of Parasutterella, Parasutterella secunda, was discovered, and both Parasutterella and Sutterella species were reclassified under the family Sutterellaceae.[2] (This reclassification occurred due to the similar biochemical potential in the Sutterella and Parasutterella genera different from other genera in the family Alcaligenaceae.)[2] To date, Parasutterella contains two cultured species and numerous uncultured species. Both cultured species were isolated from human feces and are considered to be part of the gut microbiome in a healthy individual.[1][2][3]

Characteristics

Parasutterella species are Gram-negative, coccobacilli (circular and rod-shaped), strictly (or obligate) anaerobic, non-motile bacteria.[1][2] When cultured, colonies from both P. excrementihominis and P. secunda appeared translucent to beige in color, convex and circular in shape, and extremely small in size.[1][2] Both species do not metabolize glucose, lactate, or succinate or produce indole or short-chain fatty acids.[1][2] Additionally, these bacteria do not reduce nitrate and are catalase-negative (inability to breakdown hydrogen peroxide into oxygen and water), urease-negative (inability to hydrolyze urea to ammonia and carbon dioxide), and oxidase-negative (inability to use oxygen).[1][2] However, these bacteria have the following enzymes: arginine dihydrolase, esterase (C4), esterase lipase, naphthol-AS-BI-phosphohydrolase, and alkaline phosphatase.[1][2] P. excrementihominis can grow in bile, although P. secunda has not been described in terms of this ability.[1] Moreover, P. excrementihominis and P. secunda are unique in terms of sequences and certain biochemical processes. For example, the major respiratory quinone of P. excrementihominis was methylmenaquinone-6 whereas the dominant respiratory quinone for P. secunda is methylmenaquinone-5.[2]

Parasutterella and Sutterella contain several similarities, including sequence homology, inability to grow in an aerobic environment, oxidase- and catalase-negative, and the dominant major quinone of either methylmenaquinone-5 or -6 or menaquinone-5 or -6. Still, Parasutterella species can be differentiated from Sutterella species in several ways. For example, colonies from Sutterella tend to appear a little larger and under the microscope, appear as bacilli or rod-shaped bacteria.[1] While both species of Parasutterella do not reduce nitrate, some species from Sutterella have the ability for nitrate reduction.[1] Both P. excrementihominis and P. secunda have the enzyme esterase, whereas no species in Sutterella to date have esterase (C4).[1] Also, the major cellular fatty acids in Parasutterella are different than those associated with Sutterella.[1][2]

Molecular taxonomy

Parasutterella has been divided into three different groups on the basis of their 16S rRNA sequences: P. excrementihominis, P. secunda, and uncultured bacterium.[2][4] In the Silva 16S rRNA reference library, each group can then be further divided into various accession numbers or operational taxonomical units (OTUs) due to slight differences in sequence.[4] P. excrementihominis has six different OTUs or strains, whereas P. secunda has three different OTUs.[4]

Associations in human health

Parasutterella is still a relatively new genus and has been exclusively published in regards to literature on the intestinal microbiome (with the exception of the first two taxonomical papers on the discovery of P. excrementihominis and P. secunda). Both P. excrementihominis and P. secunda were isolated from fecal samples from healthy Japanese individuals and have been found to be part of the intestinal microbiome in the United States.[1][2] In a study that examined the effect of introducing and reversing diet-induced obesity on the gut microbiome and metabolism in mice, Parasutterella was decreased in mice with diet-induced obesity and increased in controls and reversal of the high fat diet with a healthier alternative (normal chow).[5] Other studies have shown increases of Parasutterella with sugar[6] and alcohol consumption.[7] Additional microbiome studies have associated increased abundance of Parasutterella with dysbiosis, or a lack of diversity in the microbial composition typically in the gut.[8][9] (Dysbiosis has been correlated with intestinal and metabolic dysfunction and several diseases, including inflammatory bowel diseases and obesity.)[10] For example, Parasutterella was increased in the submucosa in the ileum of the small intestine in individuals with Crohn's disease[8] and hypertriglyceridemia-related acute necrotizing pancreatitis in rats.[9] More research is needed to further explore the role of Parasutterella species as a part of the healthy gut microbiome and in microbial dysbiosis.

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See also

References
  1. Nagai, Fumiko; Morotomi, Masami; Sakon, Hiroshi; Tanaka, Ryuichiro (2009). "Parasutterella excrementihominis gen. nov., sp. nov., a member of the family Alcaligenaceae isolated from human faeces". International Journal of Systematic and Evolutionary Microbiology. 59 (7): 1793–1797. doi:10.1099/ijs.0.002519-0. PMID 19542131.
  2. Morotomi, Masami; Nagai, Fumiko; Watanabe, Yohei (2011). "Parasutterella secunda sp. nov., isolated from human faeces and proposal of Sutterellaceae fam. nov. in the order Burkholderiales". International Journal of Systematic and Evolutionary Microbiology. 61 (3): 637–643. doi:10.1099/ijs.0.023556-0. PMID 20400667.
  3. Jeong, Ju-Yong; Park, Hee-Deung; Lee, Kyong-Hee; Weon, Hang-Yeon; Ka, Jong-Ok (2011-08-01). "Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing". The Journal of Microbiology. 49 (4): 585–94. doi:10.1007/s12275-011-0530-6. ISSN 1225-8873. PMID 21887641.
  4. "Search". www.arb-silva.de. Retrieved 2017-11-30.
  5. Zhang, Chenhong; Zhang, Menghui; Pang, Xiaoyan; Zhao, Yufeng; Wang, Linghua; Zhao, Liping (October 2012). "Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations". The ISME Journal. 6 (10): 1848–1857. doi:10.1038/ismej.2012.27. ISSN 1751-7370. PMC 3446802. PMID 22495068.
  6. Noble, Emily E.; Hsu, Ted M.; Jones, Roshonda B.; Fodor, Anthony A.; Goran, Michael I.; Kanoski, Scott E. (2017-01-01). "Early-Life Sugar Consumption Affects the Rat Microbiome Independently of Obesity". The Journal of Nutrition. 147 (1): 20–28. doi:10.3945/jn.116.238816. ISSN 0022-3166. PMC 5177734. PMID 27903830.
  7. Zhang, Xiaoxia; Wang, Hao; Yin, Peipei; Fan, Hang; Sun, Liwei; Liu, Yujun (2017-02-22). "Flaxseed oil ameliorates alcoholic liver disease via anti-inflammation and modulating gut microbiota in mice". Lipids in Health and Disease. 16 (1): 44. doi:10.1186/s12944-017-0431-8. ISSN 1476-511X. PMC 5322643. PMID 28228158.
  8. Chiodini, Rodrick J.; Dowd, Scot E.; Chamberlin, William M.; Galandiuk, Susan; Davis, Brian; Glassing, Angela (2015-07-29). "Microbial Population Differentials between Mucosal and Submucosal Intestinal Tissues in Advanced Crohn's Disease of the Ileum". PLOS One. 10 (7): e0134382. doi:10.1371/journal.pone.0134382. ISSN 1932-6203. PMC 4519195. PMID 26222621.
  9. Huang, Chunlan; Chen, Jing; Wang, Jingjing; Zhou, Hui; Lu, Yingying; Lou, Lihong; Zheng, Junyuan; Tian, Ling; Wang, Xingpeng (2017). "Dysbiosis of Intestinal Microbiota and Decreased Antimicrobial Peptide Level in Paneth Cells during Hypertriglyceridemia-Related Acute Necrotizing Pancreatitis in Rats". Frontiers in Microbiology. 8: 776. doi:10.3389/fmicb.2017.00776. ISSN 1664-302X. PMC 5415626. PMID 28522995.
  10. Carding, Simon; Verbeke, Kristin; Vipond, Daniel T.; Corfe, Bernard M.; Owen, Lauren J. (2015). "Dysbiosis of the gut microbiota in disease". Microbial Ecology in Health & Disease. 26: 26191. doi:10.3402/mehd.v26.26191. PMC 4315779. PMID 25651997.
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