para-Nitrophenylphosphate

*para*-Nitrophenylphosphate
Skeletal formula of para-nitrophenylphosphate	Space-filling model of the para-nitrophenylphosphate molecule
Names
	IUPAC name (4-nitrophenyl) dihydrogen phosphate
	Other names pNPP
Identifiers
CAS Number	330-13-2 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:17440 ;
ChEMBL	ChEMBL24231 ;
ChemSpider	369 ;
ECHA InfoCard	100.005.777
PubChem CID	378;
CompTox Dashboard (EPA)	DTXSID60861876 ;
	InChI InChI=1S/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12) Key: XZKIHKMTEMTJQX-UHFFFAOYSA-N ; InChI=1/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12) Key: XZKIHKMTEMTJQX-UHFFFAOYAU;
	SMILES C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)O;
Properties
Chemical formula	C6H6NO6P
Molar mass	219.09
Related compounds
Related compounds	Paraoxon
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.[1] Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm.[2] This property can be used to determine the activity of various phosphatases including alkaline phosphatase (AP) and protein tyrosine phosphatase (PTP). The substance is sensitive to light, and thus should be stored protected from light. This is also important after adding the substrate to the mixture and before reading. −20°C is the optimal storage temperature.[3]

References

Lorenz, Ulrike (2017-05-07). "Protein Tyrosine Phosphatase Assays". Current Protocols in Immunology. 91 (1): 11.7.1-12. doi:10.1002/0471142735.im1107s93. ISBN 978-0471142737. PMC 3097125. PMID 21462163.
MacKintosh, C. (1993). In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach. 221. New York: IRL Press.
Biolabs, New England. "p-Nitrophenyl Phosphate (PNPP) - NEB". www.NEB.com. Retrieved 29 October 2017.

External links

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[1] Lorenz, Ulrike (2017-05-07). "Protein Tyrosine Phosphatase Assays". Current Protocols in Immunology. 91 (1): 11.7.1-12. doi:10.1002/0471142735.im1107s93. ISBN 978-0471142737. PMC 3097125. PMID 21462163.

[2] MacKintosh, C. (1993). In D.G. Hardie (Ed.). Protein Phosphorylation: A Practical Approach. 221. New York: IRL Press.

[3] Biolabs, New England. "p-Nitrophenyl Phosphate (PNPP) - NEB". www.NEB.com. Retrieved 29 October 2017.