CYTH1

Cytohesin-1 formerly known as Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a protein that in humans is encoded by the CYTH1 gene.[5][6][7]

CYTH1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1BC9, 4A4P
Identifiers
Aliases	CYTH1, B2-1, CYTOHESIN-1, D17S811E, PSCD1, SEC7, cytohesin 1
External IDs	OMIM: 182115 MGI: 1334257 HomoloGene: 31262 GeneCards: CYTH1
Gene location (Human) Chr. Chromosome 17 (human)[1] Band 17q25.3 Start 78,674,048 bp[1] End 78,782,297 bp[1]
Gene location (Mouse) Chr. Chromosome 11 (mouse)[2] Band 11|11 E2 Start 118,132,019 bp[2] End 118,248,592 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • guanyl-nucleotide exchange factor activity • lipid binding • ARF guanyl-nucleotide exchange factor activity Cellular component • cytoplasm • extrinsic component of cytoplasmic side of plasma membrane • cell membrane • membrane • cytosol • Golgi membrane • adherens junction • bicellular tight junction • cell junction Biological process • regulation of ARF protein signal transduction • regulation of cell adhesion • vesicle-mediated transport • establishment of epithelial cell polarity Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9267	19157
Ensembl	ENSG00000108669	ENSMUSG00000017132
UniProt	Q15438	Q9QX11
RefSeq (mRNA)	NM_001292018 NM_001292019 NM_004762 NM_017456 NM_001365037 NM_001365038 NM_001365039 NM_001365040 NM_001365041	NM_001112699 NM_001112700 NM_011180 NM_001378885 NM_001378886 NM_001378887 NM_001378888
RefSeq (protein)	NP_001278947 NP_001278948 NP_004753 NP_059430 NP_001351966 NP_001351967 NP_001351968 NP_001351969 NP_001351970	NP_001106169 NP_001106170 NP_035310 NP_001365814 NP_001365815 NP_001365816 NP_001365817
Location (UCSC)	Chr 17: 78.67 – 78.78 Mb	Chr 11: 118.13 – 118.25 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Cytohesin-1 (CYTH1) is a member of the cytohesin family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of CYTHs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The CYTH1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. CYTH1 protein is 83% homologous to CYTH2.[7]

Interactions

CYTH1 has been shown to interact with:

• ARFRP1,[8]
• CD18,[9][10] and
• TRIM23.[11]

References

1. GRCh38: Ensembl release 89: ENSG00000108669 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000017132 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liu L, Pohajdak B (September 1992). "Cloning and sequencing of a human cDNA from cytolytic NK/T cells with homology to yeast SEC7". Biochim. Biophys. Acta. 1132 (1): 75–8. doi:10.1016/0167-4781(92)90055-5. PMID 1511013.
6. Dixon B, Mansour M, Pohajdak B (April 1993). "Assignment of human B2-1 gene (D17S811E) to chromosome 17qter by PCR analysis of somatic cell hybrids and fluorescence in situ hybridization". Cytogenet. Cell Genet. 63 (1): 42–4. doi:10.1159/000133498. PMID 8449036.
7. "Entrez Gene: PSCD1 pleckstrin homology, Sec7 and coiled-coil domains 1(cytohesin 1)".
8. Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S, Massmann S, Schilf C, Subauste MC, Voss M, Jakobs KH, Joost HG (April 1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D". J. Biol. Chem. 274 (14): 9744–51. doi:10.1074/jbc.274.14.9744. PMID 10092663.
9. Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (October 1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". J. Biol. Chem. 273 (42): 27459–66. doi:10.1074/jbc.273.42.27459. PMID 9765275.
10. Geiger C, Nagel W, Boehm T, van Kooyk Y, Figdor CG, Kremmer E, Hogg N, Zeitlmann L, Dierks H, Weber KS, Kolanus W (June 2000). "Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1". EMBO J. 19 (11): 2525–36. doi:10.1093/emboj/19.11.2525. PMC 212768. PMID 10835351.
11. Vitale N, Pacheco-Rodriguez G, Ferrans VJ, Riemenschneider W, Moss J, Vaughan M (July 2000). "Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)". J. Biol. Chem. 275 (28): 21331–9. doi:10.1074/jbc.M909642199. PMID 10748148.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Kolanus W, Nagel W, Schiller B, Zeitlmann L, Godar S, Stockinger H, Seed B (1996). "Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1, a cytoplasmic regulatory molecule". Cell. 86 (2): 233–42. doi:10.1016/S0092-8674(00)80095-1. PMID 8706128.
• Meacci E, Tsai SC, Adamik R, Moss J, Vaughan M (1997). "Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor". Proc. Natl. Acad. Sci. U.S.A. 94 (5): 1745–8. doi:10.1073/pnas.94.5.1745. PMC 19987. PMID 9050849.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Nagel W, Zeitlmann L, Schilcher P, Geiger C, Kolanus J, Kolanus W (1998). "Phosphoinositide 3-OH kinase activates the beta2 integrin adhesion pathway and induces membrane recruitment of cytohesin-1". J. Biol. Chem. 273 (24): 14853–61. doi:10.1074/jbc.273.24.14853. PMID 9614087.
• Betz SF, Schnuchel A, Wang H, Olejniczak ET, Meadows RP, Lipsky BP, Harris EA, Staunton DE, Fesik SW (1998). "Solution structure of the cytohesin-1 (B2-1) Sec7 domain and its interaction with the GTPase ADP ribosylation factor 1". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 7909–14. doi:10.1073/pnas.95.14.7909. PMC 20903. PMID 9653114.
• Nagel W, Schilcher P, Zeitlmann L, Kolanus W (1998). "The PH domain and the polybasic c domain of cytohesin-1 cooperate specifically in plasma membrane association and cellular function". Mol. Biol. Cell. 9 (8): 1981–94. doi:10.1091/mbc.9.8.1981. PMC 25450. PMID 9693361.
• Pacheco-Rodriguez G, Meacci E, Vitale N, Moss J, Vaughan M (1998). "Guanine nucleotide exchange on ADP-ribosylation factors catalyzed by cytohesin-1 and its Sec7 domain". J. Biol. Chem. 273 (41): 26543–8. doi:10.1074/jbc.273.41.26543. PMID 9756891.
• Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". J. Biol. Chem. 273 (42): 27459–66. doi:10.1074/jbc.273.42.27459. PMID 9765275.
• Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S, Massmann S, Schilf C, Subauste MC, Voss M, Jakobs KH, Joost HG (1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D". J. Biol. Chem. 274 (14): 9744–51. doi:10.1074/jbc.274.14.9744. PMID 10092663.
• Pacheco-Rodriguez G, Patton WA, Adamik R, Yoo HS, Lee FJ, Zhang GF, Moss J, Vaughan M (1999). "Structural elements of ADP-ribosylation factor 1 required for functional interaction with cytohesin-1". J. Biol. Chem. 274 (18): 12438–44. doi:10.1074/jbc.274.18.12438. PMID 10212218.
• Venkateswarlu K, Gunn-Moore F, Tavaré JM, Cullen PJ (1999). "EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain". J. Cell Sci. 112 ( Pt 12) (12): 1957–65. PMID 10341214.
• Ogasawara M, Kim SC, Adamik R, Togawa A, Ferrans VJ, Takeda K, Kirby M, Moss J, Vaughan M (2000). "Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors". J. Biol. Chem. 275 (5): 3221–30. doi:10.1074/jbc.275.5.3221. PMID 10652308.
• Vitale N, Pacheco-Rodriguez G, Ferrans VJ, Riemenschneider W, Moss J, Vaughan M (2000). "Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)". J. Biol. Chem. 275 (28): 21331–9. doi:10.1074/jbc.M909642199. PMID 10748148.
• Lee SY, Mansour M, Pohajdak B (2000). "B2-1, a Sec7- and pleckstrin homology domain-containing protein, localizes to the Golgi complex". Exp. Cell Res. 256 (2): 515–21. doi:10.1006/excr.2000.4845. PMID 10772823.
• Geiger C, Nagel W, Boehm T, van Kooyk Y, Figdor CG, Kremmer E, Hogg N, Zeitlmann L, Dierks H, Weber KS, Kolanus W (2000). "Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1". EMBO J. 19 (11): 2525–36. doi:10.1093/emboj/19.11.2525. PMC 212768. PMID 10835351.
• Dierks H, Kolanus J, Kolanus W (2001). "Actin cytoskeletal association of cytohesin-1 is regulated by specific phosphorylation of its carboxyl-terminal polybasic domain". J. Biol. Chem. 276 (40): 37472–81. doi:10.1074/jbc.M101502200. PMID 11438522.
• Tang P, Cheng TP, Agnello D, Wu CY, Hissong BD, Watford WT, Ahn HJ, Galon J, Moss J, Vaughan M, O'Shea JJ, Gadina M (2002). "Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2625–9. doi:10.1073/pnas.052712999. PMC 122398. PMID 11867758.