POLR2L

DNA-directed RNA polymerases I, II, and III subunit RPABC5 is a protein that in humans is encoded by the POLR2L gene.[5]

POLR2L
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5FLM, 5IYD, 5IYC, 5IY8, 5IYA, 5IYB, 5IY6, 5IY9, 5IY7
Identifiers
Aliases	POLR2L, RBP10, RPABC5, RPB10, RPB10beta, RPB7.6, hRPB7.6, polymerase (RNA) II subunit L, RNA polymerase II subunit L, RNA polymerase II, I and III subunit L
External IDs	OMIM: 601189 MGI: 1913741 HomoloGene: 129854 GeneCards: POLR2L
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p15.5 Start 837,356 bp[1] End 842,529 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 F5 Start 141,471,860 bp[2] End 141,475,132 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • zinc ion binding • RNA polymerase II activity • metal ion binding • RNA polymerase III activity • DNA-directed 5'-3' RNA polymerase activity • GO:0001948 protein binding • RNA polymerase I activity Cellular component • cytosol • nucleoplasm • DNA-directed RNA polymerase I complex • DNA-directed RNA polymerase III complex • DNA-directed RNA polymerase II, core complex • cell nucleus Biological process • termination of RNA polymerase I transcription • mRNA splicing, via spliceosome • positive regulation of gene expression, epigenetic • regulation of transcription from RNA polymerase I promoter • transcription initiation from RNA polymerase I promoter • transcription elongation from RNA polymerase II promoter • 7-methylguanosine mRNA capping • transcription from RNA polymerase II promoter • transcription-coupled nucleotide-excision repair • transcription initiation from RNA polymerase II promoter • fibroblast growth factor receptor signaling pathway • transcription from RNA polymerase III promoter • snRNA transcription from RNA polymerase II promoter • RNA metabolic process • regulation of gene silencing by miRNA • transcription, DNA-templated • transcription elongation from RNA polymerase I promoter • positive regulation of type I interferon production • somatic stem cell population maintenance • positive regulation of viral transcription Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5441	66491
Ensembl	ENSG00000177700	ENSMUSG00000038489
UniProt	P62875	P62876
RefSeq (mRNA)	NM_021128	NM_025593
RefSeq (protein)	NP_066951	NP_079869
Location (UCSC)	Chr 11: 0.84 – 0.84 Mb	Chr 7: 141.47 – 141.48 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases.[6]

Interactions

POLR2L has been shown to interact with POLR2C,[7] POLR2A,[7] POLR2B[7] and POLR2E.[7]

References

1. GRCh38: Ensembl release 89: ENSG00000177700 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000038489 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Acker J, Murroni O, Mattei MG, Kedinger C, Vigneron M (September 1996). "The gene (POLR2L) encoding the hRPB7.6 subunit of human RNA polymerase". Genomics. 32 (1): 86–90. doi:10.1006/geno.1996.0079. PMID 8786124.
6. "Entrez Gene: POLR2L polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa".
7. Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (July 1997). "Interactions between the human RNA polymerase II subunits". J. Biol. Chem. 272 (27): 16815–21. doi:10.1074/jbc.272.27.16815. PMID 9201987.

Further reading

• Jeang KT (1998). "Tat, Tat-associated kinase, and transcription". J. Biomed. Sci. 5 (1): 24–7. doi:10.1007/BF02253352. PMID 9570510.
• Yankulov K, Bentley D (1998). "Transcriptional control: Tat cofactors and transcriptional elongation". Curr. Biol. 8 (13): R447–9. doi:10.1016/S0960-9822(98)70289-1. PMID 9651670.
• Romano G, Kasten M, De Falco G, Micheli P, Khalili K, Giordano A (2000). "Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression". J. Cell. Biochem. 75 (3): 357–68. doi:10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K. PMID 10536359.
• Marcello A, Zoppé M, Giacca M (2002). "Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator". IUBMB Life. 51 (3): 175–81. doi:10.1080/152165401753544241. PMID 11547919.
• Stevens M, De Clercq E, Balzarini J (2007). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Med Res Rev. 26 (5): 595–625. doi:10.1002/med.20081. PMC 7168390. PMID 16838299.
• Harrich D, McMillan N, Munoz L, Apolloni A, Meredith L (2007). "Will diverse Tat interactions lead to novel antiretroviral drug targets?". Current Drug Targets. 7 (12): 1595–606. doi:10.2174/138945006779025338. PMID 17168834.
• Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". J. Acquir. Immune Defic. Syndr. 5 (11): 1142–7. PMID 1403646.
• Kato H, Sumimoto H, Pognonec P, Chen CH, Rosen CA, Roeder RG (1992). "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes Dev. 6 (4): 655–66. doi:10.1101/gad.6.4.655. PMID 1559613.
• Southgate C, Zapp ML, Green MR (1990). "Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein". Nature. 345 (6276): 640–2. Bibcode:1990Natur.345..640S. doi:10.1038/345640a0. PMID 2190099.
• Wu-Baer F, Sigman D, Gaynor RB (1995). "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat". Proc. Natl. Acad. Sci. U.S.A. 92 (16): 7153–7. Bibcode:1995PNAS...92.7153W. doi:10.1073/pnas.92.16.7153. PMC 41297. PMID 7638159.
• Shpakovski GV, Acker J, Wintzerith M, Lacroix JF, Thuriaux P, Vigneron M (1995). "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae". Mol. Cell. Biol. 15 (9): 4702–10. doi:10.1128/mcb.15.9.4702. PMC 230713. PMID 7651387.
• Herrmann CH, Rice AP (1995). "Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor". J. Virol. 69 (3): 1612–20. doi:10.1128/JVI.69.3.1612-1620.1995. PMC 188757. PMID 7853496.
• McKune K, Moore PA, Hull MW, Woychik NA (1996). "Six human RNA polymerase subunits functionally substitute for their yeast counterparts". Mol. Cell. Biol. 15 (12): 6895–900. doi:10.1128/mcb.15.12.6895. PMC 230944. PMID 8524256.
• Keen NJ, Gait MJ, Karn J (1996). "Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex". Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2505–10. Bibcode:1996PNAS...93.2505K. doi:10.1073/pnas.93.6.2505. PMC 39827. PMID 8637904.
• Yang X, Herrmann CH, Rice AP (1996). "The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function". J. Virol. 70 (7): 4576–84. doi:10.1128/JVI.70.7.4576-4584.1996. PMC 190394. PMID 8676484.
• Agostini I, Navarro JM, Rey F, Bouhamdan M, Spire B, Vigne R, Sire J (1996). "The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599–606. doi:10.1006/jmbi.1996.0485. PMID 8800208.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science. 274 (5287): 605–10. Bibcode:1996Sci...274..605Z. doi:10.1126/science.274.5287.605. PMID 8849451.
• Okamoto H, Sheline CT, Corden JL, Jones KA, Peterlin BM (1996). "Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II". Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11575–9. Bibcode:1996PNAS...9311575O. doi:10.1073/pnas.93.21.11575. PMC 38099. PMID 8876177.
• Chun RF, Jeang KT (1996). "Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1". J. Biol. Chem. 271 (44): 27888–94. doi:10.1074/jbc.271.44.27888. PMID 8910388.