POLR2K

DNA-directed RNA polymerases I, II, and III subunit RPABC4 is a protein that in humans is encoded by the POLR2K gene.

POLR2K
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5FLM, 5IY9, 5IYA, 5IYC, 5IYB, 5IY7, 5IY8, 5IYD, 5IY6
Identifiers
Aliases	POLR2K, ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.0, hRPB7.0, hsRPB10a, polymerase (RNA) II subunit K, RNA polymerase II subunit K, RNA polymerase II, I and III subunit K
External IDs	OMIM: 606033 MGI: 102725 HomoloGene: 86049 GeneCards: POLR2K
Gene location (Human) Chr. Chromosome 8 (human)[1] Band 8q22.2 Start 100,150,623 bp[1] End 100,154,003 bp[1]
Gene location (Mouse) Chr. Chromosome 15 (mouse)[2] Band 15|15 B3.1 Start 36,174,010 bp[2] End 36,177,010 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • zinc ion binding • RNA polymerase II activity • metal ion binding • RNA polymerase III activity • DNA-directed 5'-3' RNA polymerase activity • RNA polymerase I activity Cellular component • cytosol • nucleoplasm • DNA-directed RNA polymerase I complex • DNA-directed RNA polymerase III complex • DNA-directed RNA polymerase II, core complex • cell nucleus Biological process • termination of RNA polymerase I transcription • mRNA splicing, via spliceosome • positive regulation of gene expression, epigenetic • regulation of transcription from RNA polymerase I promoter • transcription initiation from RNA polymerase I promoter • transcription elongation from RNA polymerase II promoter • 7-methylguanosine mRNA capping • transcription from RNA polymerase II promoter • transcription-coupled nucleotide-excision repair • transcription initiation from RNA polymerase II promoter • transcription from RNA polymerase III promoter • snRNA transcription from RNA polymerase II promoter • fibroblast growth factor receptor signaling pathway • RNA metabolic process • regulation of gene silencing by miRNA • transcription, DNA-templated • transcription elongation from RNA polymerase I promoter • positive regulation of type I interferon production • somatic stem cell population maintenance • positive regulation of viral transcription Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5440	17749
Ensembl	ENSG00000147669	ENSMUSG00000045996
UniProt	P53803	Q63871
RefSeq (mRNA)	NM_005034	NM_001039368 NM_023127
RefSeq (protein)	NP_005025	NP_001034457 NP_075616
Location (UCSC)	Chr 8: 100.15 – 100.15 Mb	Chr 15: 36.17 – 36.18 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases.[5]

Interactions

POLR2K has been shown to interact with POLR2C.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000147669 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000045996 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: POLR2K polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa".
6. Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (July 1997). "Interactions between the human RNA polymerase II subunits". The Journal of Biological Chemistry. 272 (27): 16815–21. doi:10.1074/jbc.272.27.16815. PMID 9201987.

Further reading

• Jeang KT (1998). "Tat, Tat-associated kinase, and transcription". Journal of Biomedical Science. 5 (1): 24–7. doi:10.1007/BF02253352. PMID 9570510.
• Yankulov K, Bentley D (June 1998). "Transcriptional control: Tat cofactors and transcriptional elongation". Current Biology. 8 (13): R447-9. doi:10.1016/S0960-9822(98)70289-1. PMID 9651670.
• Romano G, Kasten M, De Falco G, Micheli P, Khalili K, Giordano A (December 1999). "Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression". Journal of Cellular Biochemistry. 75 (3): 357–68. doi:10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K. PMID 10536359.
• Marcello A, Zoppé M, Giacca M (March 2001). "Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator". IUBMB Life. 51 (3): 175–81. doi:10.1080/152165401753544241. PMID 11547919.
• Howe KJ (September 2002). "RNA polymerase II conducts a symphony of pre-mRNA processing activities". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1577 (2): 308–24. doi:10.1016/s0167-4781(02)00460-8. PMID 12213660.
• Stevens M, De Clercq E, Balzarini J (September 2006). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Medicinal Research Reviews. 26 (5): 595–625. doi:10.1002/med.20081. PMC 7168390. PMID 16838299.
• Harrich D, McMillan N, Munoz L, Apolloni A, Meredith L (December 2006). "Will diverse Tat interactions lead to novel antiretroviral drug targets?". Current Drug Targets. 7 (12): 1595–606. doi:10.2174/138945006779025338. PMID 17168834.
• Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.
• Kato H, Sumimoto H, Pognonec P, Chen CH, Rosen CA, Roeder RG (April 1992). "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes & Development. 6 (4): 655–66. doi:10.1101/gad.6.4.655. PMID 1559613.
• Southgate C, Zapp ML, Green MR (June 1990). "Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein". Nature. 345 (6276): 640–2. Bibcode:1990Natur.345..640S. doi:10.1038/345640a0. PMID 2190099.
• Wu-Baer F, Sigman D, Gaynor RB (August 1995). "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat". Proceedings of the National Academy of Sciences of the United States of America. 92 (16): 7153–7. Bibcode:1995PNAS...92.7153W. doi:10.1073/pnas.92.16.7153. PMC 41297. PMID 7638159.
• Shpakovski GV, Acker J, Wintzerith M, Lacroix JF, Thuriaux P, Vigneron M (September 1995). "Four subunits that are shared by the three classes of RNA polymerase are functionally interchangeable between Homo sapiens and Saccharomyces cerevisiae". Molecular and Cellular Biology. 15 (9): 4702–10. doi:10.1128/mcb.15.9.4702. PMC 230713. PMID 7651387.
• Herrmann CH, Rice AP (March 1995). "Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor". Journal of Virology. 69 (3): 1612–20. doi:10.1128/JVI.69.3.1612-1620.1995. PMC 188757. PMID 7853496.
• Keen NJ, Gait MJ, Karn J (March 1996). "Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex". Proceedings of the National Academy of Sciences of the United States of America. 93 (6): 2505–10. Bibcode:1996PNAS...93.2505K. doi:10.1073/pnas.93.6.2505. PMC 39827. PMID 8637904.
• Yang X, Herrmann CH, Rice AP (July 1996). "The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function". Journal of Virology. 70 (7): 4576–84. doi:10.1128/JVI.70.7.4576-4584.1996. PMC 190394. PMID 8676484.
• Agostini I, Navarro JM, Rey F, Bouhamdan M, Spire B, Vigne R, Sire J (September 1996). "The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB". Journal of Molecular Biology. 261 (5): 599–606. doi:10.1006/jmbi.1996.0485. PMID 8800208.
• Zhou Q, Sharp PA (October 1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science. 274 (5287): 605–10. Bibcode:1996Sci...274..605Z. doi:10.1126/science.274.5287.605. PMID 8849451.
• Okamoto H, Sheline CT, Corden JL, Jones KA, Peterlin BM (October 1996). "Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II". Proceedings of the National Academy of Sciences of the United States of America. 93 (21): 11575–9. Bibcode:1996PNAS...9311575O. doi:10.1073/pnas.93.21.11575. PMC 38099. PMID 8876177.
• Chun RF, Jeang KT (November 1996). "Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1". The Journal of Biological Chemistry. 271 (44): 27888–94. doi:10.1074/jbc.271.44.27888. PMID 8910388.
• Parada CA, Roeder RG (November 1996). "Enhanced processivity of RNA polymerase II triggered by Tat-induced phosphorylation of its carboxy-terminal domain". Nature. 384 (6607): 375–8. Bibcode:1996Natur.384..375P. doi:10.1038/384375a0. PMID 8934526.