PCBP1

Poly(rC)-binding protein 1 is a protein that in humans is encoded by the PCBP1 gene.[5]

PCBP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1WVN, 1ZTG, 3VKE
Identifiers
Aliases	PCBP1, HEL-S-85, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X, poly(rC) binding protein 1
External IDs	OMIM: 601209 MGI: 1345635 HomoloGene: 68506 GeneCards: PCBP1
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2p13.3 Start 70,087,477 bp[1] End 70,089,203 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6|6 D1 Start 86,524,492 bp[2] End 86,526,321 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • GO:0001948 protein binding • mRNA binding • RNA binding • nucleic acid binding • single-stranded DNA binding • sequence-specific single stranded DNA binding • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific • cadherin binding Cellular component • cytoplasm • extracellular exosome • membrane • cell nucleus • nucleoplasm • cytosol • nuclear speck • cytoplasmic ribonucleoprotein granule Biological process • mRNA splicing, via spliceosome • viral RNA genome replication • positive regulation of transcription from RNA polymerase II promoter • RNA metabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5093	23983
Ensembl	ENSG00000169564	ENSMUSG00000051695
UniProt	Q15365	P60335
RefSeq (mRNA)	NM_006196	NM_011865
RefSeq (protein)	NP_006187	NP_035995
Location (UCSC)	Chr 2: 70.09 – 70.09 Mb	Chr 6: 86.52 – 86.53 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP2 and HNRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.

It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000169564 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000051695 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Tommerup N, Leffers H (May 1997). "Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A". Genomics. 32 (2): 297–8. doi:10.1006/geno.1996.0121. PMID 8833161.
6. "Entrez Gene: PCBP1 poly(rC) binding protein 1".

Further reading

• Kiledjian M, Wang X, Liebhaber SA (1995). "Identification of two KH domain proteins in the alpha-globin mRNP stability complex". EMBO J. 14 (17): 4357–64. doi:10.1002/j.1460-2075.1995.tb00110.x. PMC 394520. PMID 7556077.
• Leffers H, Dejgaard K, Celis JE (1995). "Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains". Eur. J. Biochem. 230 (2): 447–53. doi:10.1111/j.1432-1033.1995.tb20581.x. PMID 7607214.
• Aasheim HC, Loukianova T, Deggerdal A, Smeland EB (1994). "Tissue specific expression and cDNA structure of a human transcript encoding a nucleic acid binding [oligo(dC)] protein related to the pre-mRNA binding protein K." Nucleic Acids Res. 22 (6): 959–64. doi:10.1093/nar/22.6.959. PMC 307915. PMID 8152927.
• Blyn LB, Swiderek KM, Richards O, et al. (1996). "Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry". Proc. Natl. Acad. Sci. U.S.A. 93 (20): 11115–20. doi:10.1073/pnas.93.20.11115. PMC 38293. PMID 8855318.
• Kiledjian M, DeMaria CT, Brewer G, Novick K (1997). "Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex". Mol. Cell. Biol. 17 (8): 4870–6. doi:10.1128/MCB.17.8.4870. PMC 232339. PMID 9234743.
• Wang Z, Day N, Trifillis P, Kiledjian M (1999). "An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro". Mol. Cell. Biol. 19 (7): 4552–60. doi:10.1128/MCB.19.7.4552. PMC 84253. PMID 10373504.
• Makeyev AV, Chkheidze AN, Liebhaber SA (1999). "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog". J. Biol. Chem. 274 (35): 24849–57. doi:10.1074/jbc.274.35.24849. PMID 10455157.
• Makeyev AV, Liebhaber SA (2000). "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins". Genomics. 67 (3): 301–16. doi:10.1006/geno.2000.6244. PMID 10936052.
• Suzuki H, Fukunishi Y, Kagawa I, et al. (2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Res. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
• Yeap BB, Voon DC, Vivian JP, et al. (2002). "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA". J. Biol. Chem. 277 (30): 27183–92. doi:10.1074/jbc.M202883200. PMID 12011088.
• Walter BL, Parsley TB, Ehrenfeld E, Semler BL (2002). "Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication". J. Virol. 76 (23): 12008–22. doi:10.1128/JVI.76.23.12008-12022.2002. PMC 136902. PMID 12414943.
• Giles KM, Daly JM, Beveridge DJ, et al. (2003). "The 3'-untranslated region of p21WAF1 mRNA is a composite cis-acting sequence bound by RNA-binding proteins from breast cancer cells, including HuR and poly(C)-binding protein". J. Biol. Chem. 278 (5): 2937–46. doi:10.1074/jbc.M208439200. PMID 12431987.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Pickering BM, Mitchell SA, Evans JR, Willis AE (2003). "Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo". Nucleic Acids Res. 31 (2): 639–46. doi:10.1093/nar/gkg146. PMC 140511. PMID 12527772.
• Pillai MR, Chacko P, Kesari LA, et al. (2003). "Expression of folate receptors and heterogeneous nuclear ribonucleoprotein E1 in women with human papillomavirus mediated transformation of cervical tissue to cancer". J. Clin. Pathol. 56 (8): 569–74. doi:10.1136/jcp.56.8.569. PMC 1770025. PMID 12890803.
• Adams DJ, Beveridge DJ, van der Weyden L, et al. (2004). "HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expression". J. Biol. Chem. 278 (45): 44894–903. doi:10.1074/jbc.M307782200. PMID 12933794.
• Evans JR, Mitchell SA, Spriggs KA, et al. (2003). "Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo". Oncogene. 22 (39): 8012–20. doi:10.1038/sj.onc.1206645. PMID 12970749.
• Chkheidze AN, Liebhaber SA (2003). "A novel set of nuclear localization signals determine distributions of the alphaCP RNA-binding proteins". Mol. Cell. Biol. 23 (23): 8405–15. doi:10.1128/MCB.23.23.8405-8415.2003. PMC 262676. PMID 14612387.