Optimal cutting temperature compound

Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.

Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production

Components

As stated on the bottle:

  • 10.24% polyvinyl alcohol
  • 4.26% polyethylene glycol
  • 85.5% non-reactive ingredients"[1]
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See also

References
  1. "OCT Compound, 27050, MSDS". Tedpella.com. Retrieved 2011-08-19.

Further reading
  • Turbett, Gavin; Sellner, Loryn (1997). "The use of optimal cutting temperature compound can inhibit amplification by polymerase chain reaction". Diagnostic Molecular Pathology. 6 (5): 298–303. doi:10.1097/00019606-199710000-00009. PMID 9458390.


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