Nucleobindin 1

Nucleobindin-1 (NUCB1), also known as calnuc, is a protein that in humans is encoded by the NUCB1 gene.[5][6]

NUCB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1SNL
Identifiers
Aliases	NUCB1, CALNUC, NUC, nucleobindin 1
External IDs	OMIM: 601323 MGI: 97388 HomoloGene: 4507 GeneCards: NUCB1
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q13.33 Start 48,900,312 bp[1] End 48,923,372 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 B3 Start 45,490,458 bp[2] End 45,510,406 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • calcium ion binding • metal ion binding • GO:0001948 protein binding • G-protein alpha-subunit binding Cellular component • cytoplasm • lumenal side of Golgi membrane • rough endoplasmic reticulum • membrane • cis-Golgi network • Golgi-associated vesicle • Golgi cisterna membrane • extracellular region • extrinsic component of Golgi membrane • trans-Golgi network • early endosome • extracellular exosome • cell nucleus • endoplasmic reticulum-Golgi intermediate compartment • extracellular • Golgi apparatus • endoplasmic reticulum lumen Biological process • regulation of protein targeting • response to cisplatin • post-translational modification • cellular protein metabolic process Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4924	18220
Ensembl	ENSG00000104805	ENSMUSG00000030824
UniProt	Q02818	Q02819
RefSeq (mRNA)	NM_006184	NM_001163662 NM_008749
RefSeq (protein)	NP_006175	NP_001157134 NP_032775
Location (UCSC)	Chr 19: 48.9 – 48.92 Mb	Chr 7: 45.49 – 45.51 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Structure

The human calnuc protein contains 461 amino acids and has a pI of 4.9.[7]

The protein contains the following regions and domains:

• Signal peptide[8]
• Leucine-zipper[8] The leucine-zipper of calnuc may be responsible for its dimerization.
• aa 347-389 – Nuclear localization signal
• 2 zinc binding sites[9] with high affinity (K_d = 20 nM)
• carboxypeptidase-like motifs : HFREXnH (aa 66) and HFTEXnH (aa 146)
• 2 EF-hands
• Potential anchor region[7]– 15-aa hydrophobic region at its COOH terminus that may be sufficient for membrane anchoring

References

1. GRCh38: Ensembl release 89: ENSG00000104805 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030824 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Miura K, Hirai M, Kanai Y, Kurosawa Y (Feb 1997). "Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4". Genomics. 34 (2): 181–6. doi:10.1006/geno.1996.0263. PMID 8661046.
6. "Entrez Gene: NUCB1 nucleobindin 1".
7. Lin P, Le-Niculescu H, Hofmeister R, McCaffery JM, Jin M, Hennemann H, McQuistan T, De Vries L, Farquhar MG (June 1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC 2132997. PMID 9647645.
8. Miura K, Titani K, Kurosawa Y, Kanai Y (August 1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID 1520323.
9. Kanuru M, Raman R, Aradhyam GK (January 2013). "Serine protease activity of calnuc: regulation by Zn2+ and G proteins". J. Biol. Chem. 288 (3): 1762–73. doi:10.1074/jbc.M112.382846. PMC 3548486. PMID 23195954.

Further reading

• Miura K, Titani K, Kurosawa Y, Kanai Y (1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID 1520323.
• Mochizuki N, Hibi M, Kanai Y, Insel PA (1995). "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system". FEBS Lett. 373 (2): 155–8. doi:10.1016/0014-5793(95)01031-9. PMID 7589456.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Ballif BA, Mincek NV, Barratt JT, et al. (1996). "Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein". Proc. Natl. Acad. Sci. U.S.A. 93 (11): 5544–9. doi:10.1073/pnas.93.11.5544. PMC 39283. PMID 8643612.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Kubota T, Miyauchi M, Miura K, et al. (1998). "Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma". Pathol. Int. 48 (1): 22–8. doi:10.1111/j.1440-1827.1998.tb03823.x. PMID 9589460.
• Lin P, Le-Niculescu H, Hofmeister R, et al. (1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC 2132997. PMID 9647645.
• Taniguchi N, Taniura H, Niinobe M, et al. (2000). "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm". J. Biol. Chem. 275 (41): 31674–81. doi:10.1074/jbc.M005103200. PMID 10915798.
• Weiss TS, Chamberlain CE, Takeda T, et al. (2002). "Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 14961–6. doi:10.1073/pnas.261572098. PMC 64966. PMID 11752444.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• de Alba E, Tjandra N (2004). "Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)". Biochemistry. 43 (31): 10039–49. doi:10.1021/bi049310a. PMID 15287731.
• Breuza L, Halbeisen R, Jenö P, et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46". J. Biol. Chem. 279 (45): 47242–53. doi:10.1074/jbc.M406644200. PMID 15308636.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Chen Y, Lin P, Qiu S, et al. (2007). "Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection" (PDF). Int. J. Oncol. 30 (5): 1137–44. doi:10.3892/ijo.30.5.1137. PMID 17390015.
• Tsukumo Y, Tomida A, Kitahara O, et al. (2007). "Nucleobindin 1 controls the unfolded protein response by inhibiting ATF6 activation". J. Biol. Chem. 282 (40): 29264–72. doi:10.1074/jbc.M705038200. PMID 17686766.