NHP2L1

NHP2-like protein 1 is a protein that in humans is encoded by the NHP2L1 gene.[4][5]

SNU13
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1E7K, 2JNB, 2OZB, 3SIU, 3SIV, 3JCR
Identifiers
Aliases	SNU13, 15.5K, FA-1, FA1, NHPX, OTK27, SNRNP15-5, SPAG12, SSFA1, NHP2L1, SNU13 homolog, small nuclear ribonucleoprotein (U4/U6.U5), small nuclear ribonucleoprotein 13
External IDs	OMIM: 601304 MGI: 893586 HomoloGene: 3672 GeneCards: SNU13
Gene location (Human) Chr. Chromosome 22 (human)[1] Band 22q13.2 Start 41,673,933 bp[1] End 41,690,504 bp[1]
Gene ontology Molecular function • U3 snoRNA binding • ATPase binding • U4 snRNA binding • U4atac snRNA binding • GO:0001948 protein binding • snoRNA binding • RNA binding • box C/D snoRNA binding Cellular component • nucleoplasm • spliceosomal complex • cell nucleus • small-subunit processome • U4/U6 x U5 tri-snRNP complex • nucleolus • dense fibrillar component • cytosolic large ribosomal subunit • precatalytic spliceosome • box C/D snoRNP complex • U4atac snRNP • macromolecular complex • U2-type precatalytic spliceosome • ribonucleoprotein Biological process • mRNA processing • ribosome biogenesis • mRNA splicing, via spliceosome • RNA splicing • maturation of LSU-rRNA • maturation of SSU-rRNA • protein biosynthesis • rRNA processing • single fertilization • box C/D snoRNP assembly Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4809	20826
Ensembl	ENSG00000100138	n/a
UniProt	P55769	Q9D0T1
RefSeq (mRNA)	NM_001003796 NM_005008	NM_011482
RefSeq (protein)	NP_001003796 NP_004999	NP_035612
Location (UCSC)	Chr 22: 41.67 – 41.69 Mb	n/a
PubMed search	[2]	[3]
Wikidata
View/Edit Human View/Edit Mouse

Function

Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.[5]

Interactions

NHP2L1 has been shown to interact with RAD17.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000100138 - Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. Saito H, Fujiwara T, Shin S, Okui K, Nakamura Y (Jan 1997). "Cloning and mapping of a human novel cDNA (NHP2L1) that encodes a protein highly homologous to yeast nuclear protein NHP2". Cytogenetics and Cell Genetics. 72 (2–3): 191–3. doi:10.1159/000134186. PMID 8978773.
5. "Entrez Gene: NHP2L1 NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)".
6. Chang MS, Sasaki H, Campbell MS, Kraeft SK, Sutherland R, Yang CY, Liu Y, Auclair D, Hao L, Sonoda H, Ferland LH, Chen LB (Dec 1999). "HRad17 colocalizes with NHP2L1 in the nucleolus and redistributes after UV irradiation". The Journal of Biological Chemistry. 274 (51): 36544–9. doi:10.1074/jbc.274.51.36544. PMID 10593953.

Further reading

• Nottrott S, Hartmuth K, Fabrizio P, Urlaub H, Vidovic I, Ficner R, Lührmann R (Nov 1999). "Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA". The EMBO Journal. 18 (21): 6119–33. doi:10.1093/emboj/18.21.6119. PMC 1171676. PMID 10545122.
• Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (Dec 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208.
• Chang MS, Sasaki H, Campbell MS, Kraeft SK, Sutherland R, Yang CY, Liu Y, Auclair D, Hao L, Sonoda H, Ferland LH, Chen LB (Dec 1999). "HRad17 colocalizes with NHP2L1 in the nucleolus and redistributes after UV irradiation". The Journal of Biological Chemistry. 274 (51): 36544–9. doi:10.1074/jbc.274.51.36544. PMID 10593953.
• Vidovic I, Nottrott S, Hartmuth K, Lührmann R, Ficner R (Dec 2000). "Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment". Molecular Cell. 6 (6): 1331–42. doi:10.1016/S1097-2765(00)00131-3. PMID 11163207.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (Jan 2002). "Directed proteomic analysis of the human nucleolus". Current Biology. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
• Leung AK, Lamond AI (May 2002). "In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles". The Journal of Cell Biology. 157 (4): 615–29. doi:10.1083/jcb.200201120. PMC 2173864. PMID 12011111.
• Watkins NJ, Dickmanns A, Lührmann R (Dec 2002). "Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP". Molecular and Cellular Biology. 22 (23): 8342–52. doi:10.1128/MCB.22.23.8342-8352.2002. PMC 134055. PMID 12417735.
• Scherl A, Couté Y, Déon C, Callé A, Kindbeiter K, Sanchez JC, Greco A, Hochstrasser D, Diaz JJ (Nov 2002). "Functional proteomic analysis of human nucleolus". Molecular Biology of the Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biology. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMC 545604. PMID 15461802.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (Jan 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
• Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (Sep 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.